ChemicalBook > CAS DataBase List > TRIS hydrochloride

TRIS hydrochloride

Product Name
TRIS hydrochloride
CAS No.
1185-53-1
Chemical Name
TRIS hydrochloride
Synonyms
TRIS-HCL;Tris-HCI;TRIZMA HYDROCHLORIDE;TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE;TRIS-HCL BUFFER;TROMETHAMINE HYDROCHLORIDE;Tris hydrochloric acid;TRIZMA HYDROCHLORIDE REAGENT GRADE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
CBNumber
CB2677770
Molecular Formula
C4H12ClNO3
Formula Weight
157.6
MOL File
1185-53-1.mol
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TRIS hydrochloride Property

Melting point:
150-152 °C
Boiling point:
225℃[at 101 325 Pa]
Density 
1.05 g/mL at 20 °C
vapor pressure 
0Pa at 20℃
storage temp. 
room temp
solubility 
H2O: 4 M at 20 °C, clear, colorless
form 
crystalline
color 
clear colorless (40 % (w/w) in H2O) solution
PH Range
7.0 - 9.0
PH
2.5-4.0 (25℃, 4M in H2O)
pka
8.1(at 25℃)
Water Solubility 
SOLUBLE
Sensitive 
Hygroscopic
λmax
λ: 260 nm Amax: 0.12
λ: 280 nm Amax: 0.08
BRN 
3675235
Stability:
Stable. Incompatible with strong oxidizing agents, bases, moisture.
InChIKey
QKNYBSVHEMOAJP-UHFFFAOYSA-N
LogP
-3.6 at 20℃
CAS DataBase Reference
1185-53-1(CAS DataBase Reference)
EPA Substance Registry System
1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
3
RTECS 
TY2900000
F 
3
TSCA 
Yes
HS Code 
29221980
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
T3253
Product name
Trizma® hydrochloride
Purity
reagent grade, ≥99.0% (titration), crystalline
Packaging
50KG
Price
$7730
Updated
2024/03/01
Sigma-Aldrich
Product number
T15760
Product name
Trizma® hydrochloride
Purity
reagent grade, ≥99%, crystalline
Packaging
500G
Price
$113
Updated
2024/03/01
Sigma-Aldrich
Product number
T15760
Product name
Trizma® hydrochloride
Purity
reagent grade, ≥99%, crystalline
Packaging
1KG
Price
$210
Updated
2024/03/01
Sigma-Aldrich
Product number
T15760
Product name
Trizma® hydrochloride
Purity
reagent grade, ≥99%, crystalline
Packaging
5KG
Price
$895
Updated
2024/03/01
Sigma-Aldrich
Product number
T15760
Product name
Trizma® hydrochloride
Purity
reagent grade, ≥99%, crystalline
Packaging
10KG
Price
$1520
Updated
2024/03/01
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TRIS hydrochloride Chemical Properties,Usage,Production

Description

Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0. The compound is instrumental in preparing Laemmli buffer, one of the most widely used SDS-PAGE buffers, and also finds application in various custom running and loading buffers, frequently in combination with glycine and SDS. Lysis, the initial step of DNA extraction, involves a buffer containing Tris and EDTA (ethylenediaminetetraacetic acid). In this process, EDTA binds to divalent cations such as calcium and magnesium. Due to their role in maintaining cell membrane integrity, their removal by EDTA leads to membrane destabilization. Tris plays a key role as the primary buffering component, maintaining the pH of the buffer at a stable point, typically around 8.0. Additionally, Tris may interact with the LPS (lipopolysaccharide) in the membrane, contributing to further membrane destabilization. This underscores Tris HCl's significance in various laboratory procedures.

Chemical Properties

TRIS hydrochloride is a white crystalline powder that has a pKa of 8.08 at 25°C and pH range of 7.0 to 9.0 that coincides with the physiological pH of most living organisms because of which it is commonly used as a component of buffer solutions in biology, biochemistry and molecular biology applications. Tris salts are also used for crystallization of proteins at various pH values. Neither Tris base nor Tris hydrochloride by itself provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering.

Uses

Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.

Uses

TRIS hydrochloride is a high quality buffer component used in SDS-PAGE. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. It is also used in the robust generation of transgenic mice.

Application

TRIS hydrochloride is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.

Preparation

The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.

General Description

TRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Biological Activity

Commonly used laboratory reagent

Purification Methods

Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.]

TRIS hydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

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TRIS hydrochloride Suppliers

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View Lastest Price from TRIS hydrochloride manufacturers

Hebei Dangtong Import and export Co LTD
Product
TRIS hydrochloride 1185-53-1
Price
US $25.00-20.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
8000kg
Release date
2023-09-15
Apeloa production Co.,Limited
Product
Trometamol hydrochloride 1185-53-1
Price
US $0.98/g
Min. Order
1g
Purity
99%
Supply Ability
2000
Release date
2024-06-05
Hebei Guanlang Biotechnology Co., Ltd.
Product
TRIS hydrochloride 1185-53-1
Price
US $10.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10 mt
Release date
2021-05-13

1185-53-1, TRIS hydrochlorideRelated Search:

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