Broxuridine
- Product Name
- Broxuridine
- CAS No.
- 59-14-3
- Chemical Name
- Broxuridine
- Synonyms
- BDU;bromodeoxyuridine;5-BRDU;BUDR;BRUDR;BR-DU;5-bdu;5-budr;radibud;5-BrdU5G
- CBNumber
- CB2775230
- Molecular Formula
- C9H11BrN2O5
- Formula Weight
- 307.1
- MOL File
- 59-14-3.mol
Broxuridine Property
- Melting point:
- 191-194 °C (dec.)(lit.)
- alpha
- 31 º (c=1, 0.1N NaOH)
- Density
- 1.8573 (rough estimate)
- refractive index
- 22 ° (C=1, H2O)
- storage temp.
- -20°C
- solubility
- NH4OH: 0.1 M at 20 °C, clear, colorless
- pka
- 7.73±0.10(Predicted)
- form
- Crystals or Crystalline Powder
- color
- White to yellow
- Water Solubility
- 1-5 g/100 mL at 22 ºC
- Merck
- 14,1452
- BRN
- 30395
- Stability:
- Stable, but may be light sensitive. Incompatible with strong oxidizing agents.
- InChIKey
- XMJRLEURHMTTRX-UHFFFAOYSA-N
- LogP
- -0.290
- CAS DataBase Reference
- 59-14-3(CAS DataBase Reference)
- EPA Substance Registry System
- Uridine, 5-bromo-2'-deoxy- (59-14-3)
Safety
- Hazard Codes
- Xn,T
- Risk Statements
- 68-36/37/38-63-46-24-61
- Safety Statements
- 36/37/39-26-53-45-36
- WGK Germany
- 2
- RTECS
- YU7350000
- F
- 10-23
- TSCA
- Yes
- HS Code
- 29349990
- Hazardous Substances Data
- 59-14-3(Hazardous Substances Data)
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H340May cause genetic defects
- Precautionary statements
-
P201Obtain special instructions before use.
P202Do not handle until all safety precautions have been read and understood.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313IF exposed or concerned: Get medical advice/attention.
P405Store locked up.
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- B5002
- Product name
- 5-Bromo-2′-deoxyuridine
- Purity
- ≥99% (HPLC)
- Packaging
- 1g
- Price
- $151
- Updated
- 2024/03/01
- Product number
- B5002
- Product name
- 5-Bromo-2′-deoxyuridine
- Purity
- ≥99% (HPLC)
- Packaging
- 5g
- Price
- $598
- Updated
- 2024/03/01
- Product number
- 203806
- Product name
- 5-Bromo-2ʹ-deoxyuridine - CAS 59-14-3 - Calbiochem
- Purity
- Thymidineanalog.UsefulforthestudyofDNAsynthesiswhereitisincorporated
- Packaging
- 1g
- Price
- $241
- Updated
- 2024/03/01
- Product number
- 203806
- Product name
- 5-Bromo-2ʹ-deoxyuridine - CAS 59-14-3 - Calbiochem
- Purity
- Thymidineanalog.UsefulforthestudyofDNAsynthesiswhereitisincorporated
- Packaging
- 5G
- Price
- $758
- Updated
- 2024/03/01
- Product number
- 10280879001
- Product name
- 5-Bromo-2′-deoxyuridine
- Packaging
- 1g
- Price
- $119
- Updated
- 2024/03/01
Broxuridine Chemical Properties,Usage,Production
Description
5-Bromo-2'-deoxyuridine is a nitrate reductase inhibitor that prevents the reduction of nitrate to nitrite by inhibiting the enzyme nitrate reductase. 5-Bromo-2'-deoxyuridine is a genotoxic agent that has been shown to cause DNA damage and cell death in vitro. 5-Bromo-2'-deoxyuridine also binds to NMDA receptors and may be useful as a model system for studying neurodegenerative diseases.
Chemical Properties
White crystalline powder
Uses
A Thymidine analogue used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase
Uses
Research tool for measuring DNA synthesis.
Uses
5-Bromo-2'-deoxyuridine (BrdU) is a thymidine analog used to label DNA. It is incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies to target the BrdU incorporation. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
Definition
ChEBI: A pyrimidine 2'-deoxyribonucleoside compound having 5-bromouracil as the nucleobase.
General Description
White crystalline powder.
Air & Water Reactions
Water soluble.
Reactivity Profile
Broxuridine may be heat and light sensitive. .
Health Hazard
ACUTE/CHRONIC HAZARDS: When heated to decomposition Broxuridine emits very toxic fumes of bromide ion and NOx.
Fire Hazard
Flash point data for Broxuridine are not available; however, Broxuridine is probably combustible.
Biological Activity
5-bromodeoxyuridine (5-brdu) is an analog of thy that is incorporated into the dna of cells undergoing the s-phase and can be detected by monoclonal antibodies or polyclona111.
Biochem/physiol Actions
5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation, because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.
Safety Profile
Moderately toxic by subcutaneous, intravenous, intraperitoneal, and possibly other routes. Mildly toxic by ingestion. Experimental teratogenic and reproductive effects. Human mutation data reported. When heated to decomposition it emits very toxic fumes of Brand NOx
in vitro
the effect of 5-brdu on the proliferation was studied in the early phases of dedifferentiation of nicotiana glauca pith explants grown. it was found to be wholly inhibitory only when given during the first 72 h of culture, this inhibition being reversed by simultaneous addition of either deoxyeytidine or thymidine [1].
in vivo
young adult offspring mice were stained for 5-brdu using a monoclonal antibody and the peroxidase-antiperoxidase method. the distribution of immunoreactive nuclei was very similar to the previous [3h] thymidine data. the pattern of labelled nuclei at different embryonic ages followed the spatiotemporal gradients described for cortical and hippocampal neurogenesis. these data indicate that brdu can be used to map neuronal birthdates [2].
storage
-20°C
Purification Methods
Recrystallise the uridine from EtOH or 96% EtOH. It has max 279 nm at pH 7.0, and 279 nm (log 3.95) at pH 1.9. Its RF values are 0.49, 0.46 and 0.53 in n-BuOH/AcOH/H2O (4:1:1), n-BuOH/EtOH/H2O (40:11:19) and i-PrOH-25% aqueous NH3-H2O (7:1:1), respectively. [Nature 209 230 1966, Pryst.s & Sorm Collect Czech Chem Comm 29 2956 1964, Beilstein 24 III/IV 1234.]
References
[1] m. durante, c. geri, v. nuti-ronchi, g. martini, e. guillé, j. grisvard, l. giorgi, r. parenti, m. bulatti. inhibition of nicotiana glauca pich tissue proliferation through incorporation of 5-brdu into sna. cell differentiation, volume 6, issue 1, june 1977, pages 53-63
[2] del rio ja, soriano e. immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. brain res dev brain res. 1989 oct 1; 49 (2): 311-7.
Broxuridine Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Broxuridine manufacturers
- Product
- 5-Bromo-2'-deoxyuridine 59-14-3
- Price
- US $0.00-0.00/KG
- Min. Order
- 100g
- Purity
- 98%min
- Supply Ability
- 100kgs
- Release date
- 2021-09-07
- Product
- Broxuridine 59-14-3
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 50000KG/month
- Release date
- 2023-10-11
- Product
- Broxuridine 59-14-3
- Price
- US $0.00-0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 500000kg
- Release date
- 2023-03-30