ChemicalBook > CAS DataBase List > 5-Aminofluorescein

5-Aminofluorescein

Product Name
5-Aminofluorescein
CAS No.
3326-34-9
Chemical Name
5-Aminofluorescein
Synonyms
5-AF;FLUORESCEINAMINE;5-AFM;AMINOFLUORESCEIN;4-AMINOFLUORESCEIN;5-AMF;(isoMer I);5-AMINOFLUORESCEIN;1-FLUORESCEINAMINE;1-AMINOFLUORESCEIN
CBNumber
CB3254491
Molecular Formula
C20H13NO5
Formula Weight
347.32
MOL File
3326-34-9.mol
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5-Aminofluorescein Property

Melting point:
223 °C (dec.)(lit.)
Boiling point:
481.89°C (rough estimate)
Density 
1.3724 (rough estimate)
refractive index 
1.5180 (estimate)
storage temp. 
room temp
solubility 
DMF: 5 mg/ml; DMSO: 5 mg/ml
pka
9.34±0.20(Predicted)
form 
powder
color 
red-brown
Appearance
Solid Powder
Water Solubility 
Soluble in water (partly), methanol (10 mg/ml), and acetone.
λmax
496 nm
BRN 
48395
Stability:
Light and Moisture Sensitive
InChIKey
GZAJOEGTZDUSKS-UHFFFAOYSA-N
CAS DataBase Reference
3326-34-9(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy- (3326-34-9)
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Safety

Hazard Codes 
Xn
Risk Statements 
22-36/37/38
Safety Statements 
26
WGK Germany 
3
F 
10
TSCA 
Yes
HS Code 
32049090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
201626
Product name
Fluoresceinamine, isomer I
Packaging
1g
Price
$177
Updated
2025/07/31
Sigma-Aldrich
Product number
201626
Product name
Fluoresceinamine, isomer I
Packaging
5g
Price
$659
Updated
2025/07/31
TCI Chemical
Product number
A0306
Product name
5-Aminofluorescein (isomer I)
Purity
>95.0%(HPLC)(T)
Packaging
1g
Price
$164
Updated
2025/07/31
TCI Chemical
Product number
A0306
Product name
5-Aminofluorescein (isomer I)
Purity
>95.0%(HPLC)(T)
Packaging
5g
Price
$514
Updated
2025/07/31
Sigma-Aldrich
Product number
201626
Product name
Fluoresceinamine, isomer I
Packaging
250mg
Price
$66.6
Updated
2025/07/31
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5-Aminofluorescein Chemical Properties,Usage,Production

Description

5-AMF is a fluorescent probe for carboxylic acids and glutamine, and it also can be coupled to aldehydes and ketones.

Chemical Properties

Dark Red Solid

Uses

Glycosaminoglycuronans react with 5-aminofluorescein to yield fluorescent derivatives. 5-aminofluorescein is also used to prepare FITC Isomer I (F-011, F-020).

Uses

5-aminofluorescein is also used to prepare FITC Isomer I (CDX-F0011, CDX-F0020). It is a chemical used as molecular probe and important as fluorescent dye and for derivatives. Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens.

Definition

ChEBI: 5-aminofluorescein is a primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid. It derives from a fluorescein (lactone form).

Preparation

The preparation of 5-aminofluorescein is carried out either by first saponification of 5-nitrofluorescein acetates with alcohol alkali, followed by reduction of the obtained 5-nitrofluorescein in a solution of alcohol alkali with hydrazine hydrate on a nickel catalyst to obtain 5-aminofluorescein , Or by boiling in an aqueous solution of sodium sulfide. The restoration of the nitro group and the saponification of acetates in the second case occurs in one stage.

General Description

Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.

5-Aminofluorescein Preparation Products And Raw materials

Raw materials

Sodium sulfide 4-Nitrophthalic acid Resorcinol Sodium hydrosulfide

Preparation Products

Fluorescein isothiocyanate
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5-Aminofluorescein Suppliers

Americas 1 Belgium 1 Canada 1 China 206 Europe 2 Germany 3 India 6 Japan 2 South Korea 1 Switzerland 4 United Kingdom 3 United States 19 Global 249
TCI AMERICA
Tel
800-4238616
Fax
+1-888-520-1075 / +1-503-283-1987
Email
sales@tciamerica.com
Country
Americas
ProdList
23653
Advantage
75
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View Lastest Price from 5-Aminofluorescein manufacturers

Hefei Lbao Physical & Chemical Science Co.,Ltd
Product
5-Aminofluorescein 3326-34-9
Price
US $0.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
Customise
Release date
2025-11-19
Hebei Chuanghai Biotechnology Co., Ltd
Product
5-Aminofluorescein 3326-34-9
Price
US $1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10 mt
Release date
2024-11-18
HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
Product
5-Aminofluorescein 3326-34-9
Price
US $99.00-66.00/kg
Min. Order
0.001kg
Purity
99%
Supply Ability
5000
Release date
2024-08-09

3326-34-9, 5-AminofluoresceinRelated Search:

Glycine Fluorescein Sodium Fluorescein Fluorescein isothiocyanate EC 1.14.14.3 Fluorescein diacetate 6-Aminocaproic acid ALTRENOGEST CHLOROPHOSPHONAZO III Tris(hydroxymethyl)aminomethane 5-(OCTADECANOYLAMINO)FLUORESCEIN 6-Aminofluorescein 5(6)-Aminofluorescein (hexadecanoyl)aminofluorescei 5-(4,6-DICHLOROTRIAZINYL)AMINOFLUORESCEIN,5-(4,6-DICHLOROTRIAZINYL)AMINOFLUORESCEIN 5-(N-dodecanoyl)aminofluorescein,5-(N-dodecanoyl)aminofluorescein AMINO ACIDS 5-Aminofluorescein

  • 5-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one Fluoresceinamine
  • 5-amino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
  • FLUORESCEINAMINE
  • FLUORESCEINAMINE ISOMER I
  • FLUORESCEINAMINE, ISOMER 1
  • 5-amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
  • 5-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE
  • 5-AMINOFLUORESCEIN ISOMER I
  • 5-AMINOFLUORESCEIN
  • 4-AMINOFLUORESCEIN ISOMER I
  • 4-AMINOFLUORESCEIN
  • 5-AFM
  • AMINOFLUORESCEIN
  • 1-FLUORESCEINAMINE
  • 1-AMINOFLUORESCEIN
  • 9’-[9h]xanthen]-3-one,5-amino-3’,6’-dihydroxy-spiro[isobenzofuran-1(3h
  • Fluoresceinamine，isomerⅡ
  • 5-AMINOFLUORESCEIN, FOR FLUORESCENCE
  • FLUORESCEIN AMINE ISOMER I 99%
  • 4-AMINOFLUORESCEIN (ISOMER 1)
  • Fluoresceinamine isomer I, pure
  • FLUORESCENAMINEISOMERI
  • 6-Amino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
  • Fluoresceinamine I
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy-
  • 3,6-Dihydroxy-5'-aminospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-3'-one
  • (isoMer I)
  • 5-AMinofluorescein&nbsp
  • FluoresceinaMine isoMer I, pure 1GR
  • FluoresceinaMine isoMer I, pure 5GR
  • Fluoresceinamine, isomer I,5-Aminofluorescein
  • 5-Fluoresceinamine isomer I
  • 5-Aminofluorescein (isomer I)&gt
  • 5-AF
  • 5-Aminofluorescein (5-AF)
  • 5-amino-3',6'-dihydroxy-3H-spiro[2-benzofuran-1, 9'-xanthen]-3-one
  • *Desflurane Impurity 6 (Desflurane EP Impurity F)
  • 5-Aminofluorescein (Fluoresceinamine, isomer I), Fluorescence marker
  • 5-Aminofluorescein, 10 mM in DMSO
  • 5-AMF
  • 3326-34-9
  • C20H13O5
  • C20H13NO5
  • Biochemicals and Reagents
  • BioChemical
  • Detection
  • Fluorescein and Derivatives
  • Fluorescent Labels
  • Fluorescent Probes, Labels, Particles and Stains
  • marker
  • Fluorescent Labels and Indicators
  • Fluorescent Labels & Indicators