Deoxyribonuclease

Product Name: Deoxyribonuclease
CAS No.: 9003-98-9
Chemical Name: Deoxyribonuclease
Synonyms: dnasw;DNASE;dnaase;DNaseA;dornase;dornava;DNASE I;DNAaseI;Dnase 1;80
CBNumber: CB3692293
Molecular Formula: NULL
Formula Weight: 0
MOL File: Mol file

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Deoxyribonuclease Property

Density: 100 g/mL at 20 °C
vapor pressure: 0.004Pa at 25℃
storage temp.: -20°C
solubility: Soluble in 0.15 M sodium chloride (5.0mg/L) clear.
form: solution (clear, colorless)
color: white
Water Solubility: 125g/L at 25℃
Merck: 13,2923
LogP: -1.3 at 20℃
EPA Substance Registry System: Nuclease, deoxyribo- (9003-98-9)

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Safety

Safety Statements: 23-24/25-22
WGK Germany: 3
RTECS: RF0750000
F: 10-21
TSCA: Yes
HS Code: 35079090

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: D5025
Product name: Deoxyribonuclease I from bovine pancreas
Purity: TypeIV,lyophilizedpowder,≥2,000?Kunitzunits/mgprotein
Packaging: 15000Units
Price: $116
Updated: 2024/03/01

Sigma-Aldrich

Product number: D7291
Product name: Deoxyribonuclease I RNase-free solution from bovine pancreas
Packaging: 0.5mg
Price: $260
Updated: 2024/03/01

Sigma-Aldrich

Product number: D5025
Product name: Deoxyribonuclease I from bovine pancreas
Purity: TypeIV,lyophilizedpowder,≥2,000?Kunitzunits/mgprotein
Packaging: 150000units
Price: $341
Updated: 2024/03/01

Sigma-Aldrich

Product number: D5025
Product name: Deoxyribonuclease I from bovine pancreas
Purity: TypeIV,lyophilizedpowder,≥2,000?Kunitzunits/mgprotein
Packaging: 375000units
Price: $744
Updated: 2024/03/01

Sigma-Aldrich

Product number: D4527
Product name: Deoxyribonuclease I from bovine pancreas
Purity: TypeII,lyophilizedpowder,Protein≥80?%,≥2,000?units/mgprotein
Packaging: 10000units
Price: $97.3
Updated: 2024/03/01

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Deoxyribonuclease Chemical Properties,Usage,Production

Uses

Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis

Uses

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Uses

Amplification grade DNase I has been used for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase.

General Description

DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.

Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
DNase I structure resembles the structure of to exonuclease III. It includes two central ? sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.^[1]

Flammability and Explosibility

Not classified

Biochem/physiol Actions

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Clinical Use

DNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I.
Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy.

Deoxyribonuclease Preparation Products And Raw materials

Raw materials

dilute sulphuric acid

Preparation Products

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Deoxyribonuclease Suppliers

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View Lastest Price from Deoxyribonuclease manufacturers

Watson Biotechnology Co.,Ltd

Product: Deoxyribonuclease 9003-98-9
Price: US $0.00-0.00/kg
Min. Order: 10kg
Purity: 2183μ/mg
Supply Ability: 100
Release date: 2024-08-06

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Deoxyribonuclease 9003-98-9
Price: US $0.00/g
Min. Order: 1g
Purity: 99%
Supply Ability: 10kg/month
Release date: 2021-07-20

Hebei Yanxi Chemical Co., Ltd.

Product: Deoxyribonuclease 9003-98-9
Price: US $0.00/kg
Min. Order: 1kg
Purity: 0.99
Supply Ability: 20 tons
Release date: 2023-11-15

9003-98-9, DeoxyribonucleaseRelated Search:

Ribonucleic acid Nuclease, streptococcal deoxyribo- Alkaline Phosphatase Recombinant RNasin Chenodeoxycholic acid MUNG BEAN NUCLEASE Ursodeoxycholic acid Phosphatase, Acid froM Wheat GerM DEOXYRIBONUCLEIC ACID SODIUM SALT EC 3.1.31.1 Ribonuclease A DEOXYCHOLIC ACID SODIUM SALT MONOHYDRATE DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEASE II Deoxyribonucleic acid sodium salt from salmon testes Sodium deoxycholate Deoxycholic acid METHYL ISOCYANOACETATE

Deoxyribonuclease I from Bovine Pancreas(Filtered)

CHRYSENE NEAT

DeoxyribonueleaseⅠ (Dnase I)

BENZO(A)PYRENE 50UG/ML IN TOLUENE

Deoxyribonuclease I from Bovine Pancreas(Ribonuclease&Protease Free)

Deoxyribonuclease I from bovine pancreas(Recombinant)

DEOXYRIBONUCLEASE I FROM BOVINEPANCREAS STANDARDIZ

DEOXYRIBONUCLEASE I F. BOVINE PANCREAS, LYOPH. ~3000 U/MG

DEOXYRIBONUCLEASE I F. BOVINE PANCREAS, ~2000 U/MG*

DEOXYRIBONUCLEASE I, BOVINE, RECOMBINANT

DEOXYRIBONUCLEASE I F. BOVINE PANCREAS, ~1800 U/MG*

DEOXYRIBONUCLEASE I FROM BOVINE PANCREAS , 10000 U

DEOXYRIBONUCLEASE I FROM BOVINE PANCREAS , ~2500 U/MG

DEOXYRIBONUCLEASE I TYPE IV FROM*BOVINE PANCREAS

DEOXYRIBONUCLEASE I FROM BOVINE PANCREASE, TYPE II-S

DEOXYRIBONUCLEASE I TYPE II FROMBOVINE P ANCREAS

DeoxyribonucleaseIExBovinePancreas

Deoxyribonucleasefrombovinespleen

Deoxyribonucleasefrombovinepancreas

Bovine pancrease

DEOXYRIBONUCLEASE I FROM BOVINE

DNase-S recombinant, min. 500.000 U/mg

Deoxyribonuclease I froM Bovine Pancreas(RNase & Protease Free,Solution)

Deoxyribonuclease I from Bovine Pancreas(Recombinant,Solution)

Deoxyribonucleate 6′-oligonucleotido-hydrolase

deoxyribonuclease Deoxyribonucleate 5′-oligonucleotido-hydrolase DNase I

Deoxyribonuclease I, from bovine pancreas, >=2000 KU/mg

DeoxyribonueleaseⅠ

5'-OLIGO-NUCLEOTIDE HYDROLASE

deoxyribonuclease I type ii-S from*bovine pancrea

Nuclease, deoxyribo-

deoxyribonucleasea

Deoxyribonucleasebovinepancreas

deoxyribonucleaseif.bovinepancreas

deoxyribonucleicphosphatase

dnaase

dnadepolymerase

dnaendonuclease

dnanuclease

dnasw

dornase

dornava

dornavac

endodeoxyribonucleasei

escherichiacoliendonucleasei

pancreaticdornase

IUB: 3.1.21.1

DEOXYRIBONUCLEASE

DEOXYRIBONUCLEASE 1

DEOXYRIBONUCLEASE, BOVINE SPLEEN

DEOXYRIBONUCLEASE I

DEOXYRIBONUCLEASE I, BOVINE

DEOXYRIBONUCLEASE I, BOVINE PANCREAS

DEOXYRIBONUCLEASE I DN-25

DEOXYRIBONUCLEASE I DN-EP

DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE

DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDO-HYDROLASE, BOVINE

DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE DN-25