N-[3-[[5-Iodo-4-[[3-[(2-thienylcarbonyl)amino]propyl]amino]-2-pyrimidinyl]amino]phenyl]-1-pyrrolidinecarboxamide

N-[3-[[5-Iodo-4-[[3-[(2-thienylcarbonyl)amino]propyl]amino]-2-pyrimidinyl]amino]phenyl]-1-pyrrolidinecarboxamide Property

Melting point:

>163°C (dec.)

Density 

1.644

storage temp. 

2-8°C

solubility 

DMSO: soluble15mg/mL, clear

form 

powder

pka

12.57±0.70(Predicted)

color 

white to light brown

Stability:

Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months.

InChIKey

VAVXGGRQQJZYBL-UHFFFAOYSA-N

Safety

WGK Germany 

3

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

N-[3-[[5-Iodo-4-[[3-[(2-thienylcarbonyl)amino]propyl]amino]-2-pyrimidinyl]amino]phenyl]-1-pyrrolidinecarboxamide Chemical Properties,Usage,Production

Description

3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a serine-threonine kinase that phosphorylates and activates a range of other kinases, including PKB, PKA, and certain isoforms of PKC. BX-795 is a potent, ATP-competitive inhibitor of PDK1 in vitro (IC₅₀ = 11 nM) and in cells (IC₅₀ = 300 nM). At comparable concentrations, BX-795 also inhibits ERK8, MAPK-interacting kinase 2, Aurora B, Aurora C, MAP/microtubule affinity-regulating kinases 1-4, TNF receptor associated factor-associated NF-κB activator-binding kinase 1, IκB kinase ε, and additional kinases.

Uses

BX-795 hydrochloride has been used to study the effect of kinase inhibition on human endogenous retroviruses (HERVs) transcription activation.

Definition

ChEBI: N-[3-[[5-iodo-4-[3-[[oxo(thiophen-2-yl)methyl]amino]propylamino]-2-pyrimidinyl]amino]phenyl]-1-pyrrolidinecarboxamide is a member of ureas.

Biochem/physiol Actions

BX-795 competes for the ATP (adenosine triphosphate) binding pocket of 3-phosphoinositide-dependent kinase-1 (PDK1) with its substrate ATP. In vitro assays reveal that BX-795 might inhibit Unc-51 (serine/threonine-protein kinase)-like autophagy activating kinase (ULK1).

storage

Store at -20°C

References

[1] RICHARD I FELDMAN. Novel small molecule inhibitors of 3-phosphoinositide-dependent kinase-1.[J]. The Journal of Biological Chemistry, 2005, 280 20: 19867-19874. DOI:10.1074/jbc.m501367200
[2] JENNY BAIN. The selectivity of protein kinase inhibitors: a further update.[J]. Biochemical Journal, 2007: 297-315. DOI:10.1042/bj20070797
[3] KRISTOPHER CLARK. Use of the pharmacological inhibitor BX795 to study the regulation and physiological roles of TBK1 and IkappaB kinase epsilon: a distinct upstream kinase mediates Ser-172 phosphorylation and activation.[J]. The Journal of Biological Chemistry, 2009, 284 21: 14136-14146. DOI:10.1074/jbc.m109.000414
[4] LI-YUAN BAI . BX795, a TBK1 inhibitor, exhibits antitumor activity in human oral squamous cell carcinoma through apoptosis induction and mitotic phase arrest[J]. European journal of pharmacology, 2015, 769: Pages 287-296. DOI:10.1016/j.ejphar.2015.11.032
[5] EUN A CHOI . A pharmacogenomic analysis using L1000CDS2 identifies BX-795 as a potential anticancer drug for primary pancreatic ductal adenocarcinoma cells[J]. Cancer letters, 2019, 465: Pages 82-93. DOI:10.1016/j.canlet.2019.08.002
[6] SARAH A SCUDERI. TBK1 Inhibitor Exerts Antiproliferative Effect on Glioblastoma Multiforme Cells.[J]. Oncology Research, 2021, 28 7: 779-790. DOI:10.3727/096504021x16161478258040
[7] TOLGA SUTLU. Inhibition of intracellular antiviral defense mechanisms augments lentiviral transduction of human natural killer cells: implications for gene therapy.[J]. Human gene therapy, 2012, 23 10: 1090-1100. DOI:10.1089/hum.2012.080
[8] DAVID S J ALLAN. Systematic improvements in lentiviral transduction of primary human natural killer cells undergoing ex vivo expansion.[J]. Molecular Therapy. Methods & Clinical Development, 2021: 559-571. DOI:10.1016/j.omtm.2021.01.008
[9] PETER CHOCKLEY  Stephen G  Sagar L Patil. Transient blockade of TBK1/IKKε allows efficient transduction of primary human natural killer cells with vesicular stomatitis virus G-pseudotyped lentiviral vectors[J]. Cytotherapy, 2021, 23 9: Pages 787-792. DOI:10.1016/j.jcyt.2021.04.010
[10] LINGYU LI. Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKKɛ complex inhibitor BX795.[J]. Journal of Translational Medicine, 2020: 363. DOI:10.1186/s12967-020-02526-2