ChemicalBook > CAS DataBase List > Papain

Papain

Product Name
Papain
CAS No.
9001-73-4
Chemical Name
Papain
Synonyms
arbuz;apain;Velardon;IMMOBILIZED PAPAIN;Papain, Suspension;Papain;caroid;Papase;Vermizym;papainum
CBNumber
CB6135333
Molecular Formula
C9H14N4O3
Formula Weight
226.23246
MOL File
9001-73-4.mol
More
Less

Papain Property

Flash point:
29 °C
storage temp. 
2-8°C
solubility 
H2O: soluble1.2mg/mL
form 
lyophilized powder
color 
almost white
Water Solubility 
Soluble in water, insoluble in most organic solvents.
Merck 
7016
Stability:
Hygroscopic
InChI
InChI=1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChIKey
CQOVPNPJLQNMDC-UHFFFAOYSA-N
SMILES
C(O)(=O)C(NC(=O)CCN)CC1NC=NC=1
EPA Substance Registry System
Papain (9001-73-4)
More
Less

Safety

Hazard Codes 
Xn
Risk Statements 
42/43-42-36/37/38-20/21/22-10
Safety Statements 
36-36/37-26-24-22-45-23-36/37/39
WGK Germany 
3
RTECS 
RU4950000
TSCA 
Yes
HS Code 
35079090
Toxicity
TDLo orl-man: 71 mg/kg:GIT JCGADC 9,127,87
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P501Dispose of contents/container to..…

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
1495005
Product name
Papain from Carica papaya
Purity
United States Pharmacopeia (USP) Reference Standard
Packaging
1g
Price
$544
Updated
2024/03/01
Sigma-Aldrich
Product number
10108014001
Product name
Papain
Purity
from Carica papaya
Packaging
10ml
Price
$117
Updated
2024/03/01
Sigma-Aldrich
Product number
5125-50GM-M
Product name
Papain, Carica papaya
Packaging
50g
Price
$86.1
Updated
2022/05/15
Alfa Aesar
Product number
J61875
Product name
Papain, Carica Papaya Latex
Packaging
25mg
Price
$94.2
Updated
2023/06/20
Alfa Aesar
Product number
J61875
Product name
Papain, Carica Papaya Latex
Packaging
1g
Price
$992
Updated
2023/06/20
More
Less

Papain Chemical Properties,Usage,Production

Chemical Properties

cream to light brown powder

Originator

Papain,Green Cross ,Japan,1969

Uses

Papain may cause allergic manifestation .Person handling this material should be protected against inhalation of dust and contact with the skin or eyes.

Uses

For tenderizing meats; for clearing beverages; for bating skins.

Uses

papain is a papaya enzyme able to dissolve keratin. Papain is used in face masks and peeling lotions as a very gentle exfoliant. It can be irritating to the skin but less so than bromelin, a similar enzyme found in pineapples and also used in cosmetics. It is considered a non-comedogenic raw material.

Uses

Papain is a tenderizer that is a protein-digesting enzyme obtained from the papaya fruit. the enzyme, used in a patented process, is injected into the circulatory system of the live animal and is activated by the heat of cooking to break down the protein, thus tenderizing the beef. the enzyme is inactivated by stomach acids.

Definition

papain: A protein-digesting enzymeoccurring in the fruit of the West Indianpapaya tree (Carica papaya). It isused as a digestant and in the manufactureof meat tenderizers.

Application

Papain (EC 3.4.22.2) is a protease derived from the latex portion of the papaya plant ( Carica papaya ), and is gathered by scoring the surface of the mature, unripe fruit, after which the latex is collected and dried (Schwimmer, 1981) . Drying can occur by one of several methods: sun, oven, or spray drying; end products having the highest activity are obtained by spray drying. Papain has broad-spectrum enzyme activity over a wide pH and temperature range, and it cleaves at basic amino acids or those having large hydrophobic side chains. Resulting peptides having terminal hydrophobic amino acids can result in a product with a bitter aftertaste. For this reason, papain is usually combined with other enzymes in the production of flavorings. Because papain is a thiol protease, oxidation of its cysteine residue under certain conditions can reduce the enzyme’s activity or potency. For this reason papain preparations often contain reducing agents such as sodium metabisulfite. Papain is economically available commercially in both liquid and powder forms. It has the longest history of use in meat systems and is the basis for some of the popular tenderizing sprinkle-on products that can be purchased by home consumers (Enzyme Development Corporation, 1999).

Manufacturing Process

Crude papain, obtained as the dried exudate of the fruit and leaves of Carica papaya L., Caricaceae, is usually found to have been contaminated during collection, drying, or storage by insects, rodent hair and excreta, botanical plant parts, sand, etc. and may thereby become further contaminated by harmful bacteria and enteric organisms.
Heretofore papain has been purified by dispersing the crude enzymes in water, filtering and spray-drying. In this procedure, however, the soluble contaminants are retained in the dried product. It has also been known to purify papain by dispersing it in water and adding acetone to reprecipitate the enzymes leaving many of the acetone-soluble and water-soluble impurities in the supernatant liquid. The material thus purified possesses a very disagreeable sulfidelike taste probably due to the reaction between the acetone and reactive sulfhydryl groups present in the papaya latex.
It has now been found that an enzyme mixture of high purity which contains none of the objectionable sulfidelike taste can be obtained by dispersing the crude enzymes in water, adding a quantity of a water-miscible lower-alkanol to the incipient precipitation point of the proteolytic enzymes thereby retaining the maximum proteolytic activity (i.e., the maximum amount of the proteolytic enzymes) in the solvent phase while precipitating the major portion of the lower-alkanol insoluble contaminants, removing the loweralkanol insoluble contaminants and precipitated inert materials, for example, by filtration or centrifugation, and then adding an additional quantity of the water-miscible lower-alkanol sufficient to precipitate the proteolytic enzymes.
The following is a specific example of the conduct of the present process. 100 g of crude papain were stirred with 120 ml of 0.01 M cysteine hydrochloride for one hour during which time the papain was completely dispersed. To the dispersion was added slowly and with vigorous stirring 147 ml of methanol. The mixture, which contained 55% methanol by volume, was stirred for about thirty minutes and centrifuged and the clear supernatant liquid was removed and saved. The precipitate was washed with 50 ml of 55% aqueous methanol, and the mixture was centrifuged again. The precipitate containing the undesirable, insoluble contaminants was discarded, and the clear wash liquid was combined with the main supernatant. To the combined clear supernatant liquid was added slowly and with vigorous stirring 265 ml of methanol to give a mixture containing 75.5% methanol by volume. The enzymes were precipitated as a taffylike gum which was isolated by decantation of the supernatant liquid containing the undesirable, soluble contaminants and traydrying. Alternatively, the precipitated enzymes can be redissolved in pure water and spray-dried

brand name

Caroid (Sterling Winthrop).

Therapeutic Function

Enzyme, Wound adhesion inhibitor

General Description

Papain (Papase), the dried and purifiedlatex of the fruit of Carica papaya L. (Caricaceae), can digestprotein in either acidic or alkaline media; it is best at a pH between4 and 7 and at 65°C to 90°C. It occurs as light brownishgray to weakly reddish brown granules, or as a yellowishgray to weakly yellow powder. It has a characteristic odor andtaste and is incompletely soluble in water to form an opalescentsolution. The commercial material is prepared by evaporatingthe juice, but the pure enzyme has also been preparedand crystallized. In medicine, it has been used locally in variousconditions similar to those for which pepsin is used. Ithas the advantage of activity over a wider range of conditions,but it is often much less reliable. Intraperitoneal instillation ofa weak solution has been recommended to counteract a tendencyto develop adhesions after abdominal surgery, and severalenthusiastic reports have been made about its value underthese conditions. Papain has been reported to cause allergiesin persons who handle it, especially those who are exposed toinhalation of the powder.

Biological Activity

papain is a cysteine protease of the peptidase c1 family, which is used in food, pharmaceutical, textile, and cosmetic industries.

Biochem/physiol Actions

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.

Safety Profile

Poison by intraperitoneal route. Human systemic effects by ingestion: changes in structure or function of esophagus. Experimental teratogenic and reproductive effects. An allergen. When heated to decomposition it emits toxic fumes of NOx.

Purification Methods

A suspension of 50g of papain (freshly ground in a mortar) in 200mL of cold water is stirred at 4o for 4hours, then filtered through a Whatman No 1 filter paper. The clear yellow filtrate is cooled in an ice-bath while a rapid stream of H2S is passed through it for 3hours, and the suspension is centrifuged at 2000rpm for 20minutes. Sufficient cold MeOH is added slowly with stirring to the supernatant to give a final MeOH concentration of 70 vol%. The precipitate, collected by centrifugation for 20minutes at 2000rpm, is then dissolved in 200mL of cold water, the solution is saturated with H2S, centrifuged, and the enzyme is again precipitated with MeOH. The process is repeated four times. [Bennett & Niemann J Am Chem Soc 72 1798 1950.] Papain has also been purified by affinity chromatography on a column of GlyGlyTyrArg-agarose [Stewart et al. J Am Chem Soc 109 3480 1986].

Papain Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

Papain Suppliers

Wuhan DKY Technology Co.,Ltd.
Tel
27-81302488 18007166089
Fax
027-81302088
Email
info@dkybpc.com
Country
China
ProdList
2024
Advantage
58
Wuhan Xingzhongcheng Technology Co., Ltd.
Tel
027-83383318 15549490740
Fax
027-83389957
Email
358174403@qq.com
Country
China
ProdList
441
Advantage
58
ChengDu SinoStandards Bio-Tech Co.,Ltd. ,
Tel
028-83243028 19136088901
Email
sinostandards@163.com
Country
China
ProdList
4985
Advantage
58
Hubei Chenghai Chemical Co., Ltd
Tel
18327245847 18327245847
Email
1400838877@qq.com
Country
China
ProdList
9961
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
future industrial shanghai co., ltd
Tel
400-0066400 13621662912
Fax
021-55660885
Email
sales@jonln.com
Country
China
ProdList
1995
Advantage
65
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
JinYan Chemicals(ShangHai) Co.,Ltd.
Tel
13817811078
Fax
86-021-50426522,50426273
Email
sales@jingyan-chemical.com
Country
China
ProdList
9976
Advantage
60
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
XiaoGan ShenYuan ChemPharm co,ltd
Tel
15527768850
Email
1791901229@qq.com
Country
China
ProdList
8836
Advantage
52
Shandong Xiya Chemical Co., Ltd
Tel
4009903999 13355009207
Fax
0539-6365991
Email
3007715519@qq.com
Country
China
ProdList
18738
Advantage
57
Sichuan Kulinan Technology Co., Ltd
Tel
400-1166-196 18981987031
Fax
028-84555506 800101999
Email
cdhxsj@163.com
Country
China
ProdList
11707
Advantage
57
Tianjin heowns Biochemical Technology Co., Ltd.
Tel
400 638 7771
Email
sales@heowns.com
Country
China
ProdList
14435
Advantage
57
Sinopharm Chemical Reagent Co,Ltd.
Tel
86-21-63210123
Fax
86-21-63290778 86-21-63218885
Email
sj_scrc@sinopharm.com
Country
China
ProdList
9815
Advantage
79
Hangzhou Yuhao Chemical Technology Co., Ltd
Tel
0571-82693216
Fax
+86-571-82880190
Email
info@yuhaochemical.com
Country
China
ProdList
9387
Advantage
52
Spectrum Chemical Manufacturing Corp.
Tel
021-021-021-67601398-809-809-809 15221380277
Fax
021-57711696
Email
marketing_china@spectrumchemical.com
Country
China
ProdList
9658
Advantage
60
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
meilunui@163.com
Country
China
ProdList
4727
Advantage
58
ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
Fax
021-55068248
Email
shyysw007@163.com
Country
China
ProdList
4940
Advantage
60
Chengdu Ai Keda Chemical Technology Co., Ltd.
Tel
4008-755-333 18080918076
Fax
028-86757656
Email
800078821@qq.com
Country
China
ProdList
9718
Advantage
55
Thermo Fisher Scientific
Tel
800-810-5118
Fax
+86-10-84193589
Email
cnchemical@thermofisher.com
Country
China
ProdList
17770
Advantage
75
Beijing HuaMeiHuLiBiological Chemical
Tel
010-56205725
Fax
010-65763397
Email
waley188@sohu.com
Country
China
ProdList
12335
Advantage
58
9ding chemical ( Shanghai) Limited
Tel
4009209199
Fax
86-021-52271987
Email
sales@9dingchem.com
Country
China
ProdList
22514
Advantage
55
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
400-400-6206333 18521732826
Fax
021-50323701
Email
market@aladdin-e.com
Country
China
ProdList
25003
Advantage
65
The future of Shanghai Industrial Co., Ltd.
Tel
021-61552785
Fax
021-55660885
Email
sales@shshiji.com
Country
China
ProdList
9545
Advantage
55
Shanghai Ruji Biology Technology Co., Ltd.
Tel
+86-21-65211385-8001 36031160
Fax
+86-21-65211385-8004
Email
sales@shruji.com
Country
China
ProdList
3224
Advantage
55
Shanghai Tauto Biotech Co., Ltd.
Tel
021-51320588
Fax
0086-21-51320502
Email
tauto@tautobiotech.com
Country
China
ProdList
3989
Advantage
66
Nanjing Dulai Biotechnology Co., Ltd.
Tel
025-84699383-8003 18013301590
Fax
025-84699383-8003
Email
njduly@126.com
Country
China
ProdList
971
Advantage
55
Nanjing Sunlida Biological Technology Co., Ltd.
Tel
025-57798810
Fax
025-57019371
Email
sales@sunlidabio.com
Country
China
ProdList
3239
Advantage
55
TargetMol Chemicals Inc.
Tel
021-021-33632979 15002134094
Fax
021-33632979
Email
marketing@targetmol.com
Country
China
ProdList
7783
Advantage
58
Shanghai BeiZhuo Biotech Co., Ltd.
Tel
021-61119791,13386096464
Fax
021-50190009
Email
bzswkf@foxmail.com
Country
China
ProdList
3921
Advantage
50
Shanghai JONLN Reagent Co., Ltd.
Tel
400-0066400 13621662912
Fax
021-55660885
Email
422131432@qq.com
Country
China
ProdList
9978
Advantage
55
MP Biomedicals
Tel
400-1500-680 021-62402299
Fax
021-62402299
Email
nancy.cai@mpbio.com
Country
China
ProdList
125
Advantage
60
Chengdu HuaXia Chemical Reagent Co. Ltd
Tel
400-1166-196 13458535857
Fax
QQ:800101999
Email
cdhxsj@163.com
Country
China
ProdList
13350
Advantage
58
Shaanxi Pioneer Biotech Co., Ltd.
Tel
13720616393;029-84385017-8003 13720616393
Fax
029-84385017
Email
sales@pioneerbiotech.com
Country
China
ProdList
778
Advantage
56
Suzhou yacoo science co.,Ltd
Tel
0512-87182056 18013166090
Fax
0512-87182056
Email
lingling.qi@yacoo.com.cn
Country
China
ProdList
7295
Advantage
60
Shanghai Macklin Biochemical Co.,Ltd.
Tel
15221275939 15221275939
Fax
021-50706099
Email
shenlinxing@macklin.cn
Country
China
ProdList
16166
Advantage
55
Wuhan Dahua Weiye Pharmaceutical Chemical Co., Ltd.
Tel
027-59420981,18702770802
Fax
027-83322098
Country
China
ProdList
1975
Advantage
50
Chengdu RunZeBenTu Chemical Co., Ltd
Tel
13096311329 028-88469284 616445927
Fax
028-88469284
Email
616445927@qq.com
Country
China
ProdList
2874
Advantage
50
Hubei Jusheng Technology Co.,Ltd
Tel
027-59599241 18871490274
Fax
027-59599241
Email
1400878000@qq.com
Country
China
ProdList
9958
Advantage
58
Chizhou Kailong Import and Export Trade Co., Ltd.
Tel
Fax
-
Email
xg01_gj@163.com
Country
China
ProdList
9484
Advantage
50
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51456
Advantage
80
Watson Biotechnology Co.,Ltd
Tel
027-59207879 1972026995 18140587686
Fax
QQ:1972026995
Email
sales@3600chem.com
Country
China
ProdList
4661
Advantage
55
Kono Chem Co.,Ltd
Tel
15829389671
Fax
029-86107037
Email
info@konochemical.com
Country
China
ProdList
255
Advantage
55
Shanghai Synchem Pharma Co., ltd
Tel
21-619849051-1 18521059765
Email
synchempharma@aliyun.com
Country
China
ProdList
6463
Advantage
55
Beijing NuoqiYa Biotechnology Co., Ltd.
Tel
4000-819-385 010-62329685 13718666987
Fax
010-62340519
Country
China
ProdList
1971
Advantage
55
Hefei TNJ Chemical Industry Co.,Ltd.
Tel
551-65418679 15837135945
Fax
0551-65418697
Email
info@tnjchem.com
Country
China
ProdList
1889
Advantage
62
Codow Chemical Co.,Ltd.
Tel
18620099427 18620099427
Fax
+86-20-62619665
Email
amy@howeipharm.com
Country
China
ProdList
1751
Advantage
55
Shanghai Xilong Biochemical Technology Co., Ltd.
Tel
021-52907766-8042
Fax
021-52906523
Country
China
ProdList
9941
Advantage
58
Shanghai QianYan Bio-technology Co., Ltd
Tel
02781293128
Email
orders@biochemsafebuy.com
Country
China
ProdList
9923
Advantage
55
More
Less

View Lastest Price from Papain manufacturers

Hebei Zhuanglai Chemical Trading Co Ltd
Product
Papain 9001-73-4
Price
US $10.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20 ton
Release date
2024-11-14
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Papain 9001-73-4
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
100kg/month
Release date
2021-07-20
BINBO BIOLOGICAL CO.,LTD
Product
Papain 9001-73-4
Price
US $0.00/kg
Min. Order
1kg
Purity
60TU/mg-1750TU/mg
Supply Ability
3000 kg
Release date
2024-11-11

9001-73-4, PapainRelated Search:


  • Papain Coarse-enzyme
  • Papain
  • Velardon
  • Vermizym
  • Papain, for biochemistry
  • papain from carica papaya
  • papain from papaya latex
  • PAPAIN,POWDER,PURIFIED
  • PAPAIN-HYDROLYZEDPORKMEAT
  • ADOLPH'SMEATTENDERIZER
  • PAPAINPOWDER
  • papayotim
  • PAPAIN 2 X crystalline extrapure
  • papainum
  • Papayaze
  • Papain from papaya latex,Papainase
  • Papain (1 g)
  • arbuz
  • caroid
  • nematolyt
  • PapainPowder[SodiumMetabisulfiteFree]
  • P-Nitrophenyl--D-Maltohexoside
  • PapainPurified
  • PapainExPapayaLatex(E.C.3.4.22.2)
  • PapainFreezeDried
  • Papain, ≥3 units/mg
  • Papain, BC Grade
  • summetrin
  • tromasin
  • vegetablepepsin
  • Papain food grade
  • PAPAIN 70
  • PARATHYROID HORMONE FRAGMENT*NLE8,18,TYR 34-AMIDE FR
  • PAPAIN ASEPTICALLY FILLED
  • PAPAIN LYOPHILIZED
  • PAPAIN FROM CARICA PAPAYA, POWDER, ~0.5 U/MG
  • PAPAIN CRUDE
  • Papain from Carica papaya, powder, >3 U/mg
  • PAPAIN FROM CARICA PAPAYA, ~12 U/MG
  • PAPAIN FROM CARICA PAPAYA, ~20 U/MG
  • PAPAIN FROM PAPAYA JUICE
  • FRUCTUS CHAENOMELIS
  • MultiPharM (TM) Papain 6000 U/Mg, Stabilized with SodiuM Disulfite, USP
  • Papain (1 g) (COLD SHIPMENT REQUIRED)
  • IMMOBILIZED PAPAIN
  • Papain, 2500 units/Mg
  • Papain from Carica papaya Latex(Suspension)
  • Papain 2x crystallized from papaya Latex
  • Papain from Carica papaya Latex(Lyophilized)
  • Papain, 2400 units/mg
  • Papain, Suspension
  • Native Papaya latex Papain
  • Papain (Technical Grade)
  • Papain USP/EP/BP
  • papaya enzima
  • apain
  • Enzyme Papain
  • Papain 1x USP ex. Papaya Latex, 6000USP U/mg