CALCEIN-AM

ChemicalBook > CAS DataBase List > CALCEIN-AM

Product Name: CALCEIN-AM
CAS No.: 148504-34-1
Chemical Name: CALCEIN-AM
Synonyms: CAL-AM;NSC 689290;CALCEIN-AM;AM /Calcein-AM;CALCEIN, AM;CAL-AM;Calcein AM solution;Cellstain- Calcein-AM;Calcein AcetoxyMethyl Ester;-Cellstain-Calcein-AmSolution;Calcein, AM *CAS 148504-34-1*
CBNumber: CB6304173
Molecular Formula: C46H46N2O23
Formula Weight: 994.86
MOL File: 148504-34-1.mol

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CALCEIN-AM Property

Melting point:: >250℃
Boiling point:: 982.7±65.0 °C(Predicted)
Density: 1.49±0.1 g/cm3(Predicted)
refractive index: n20/D 1.479
Flash point:: 85 °C
storage temp.: -20°C
solubility: DMSO: soluble10mg/mL, clear, colorless to slightly yellow
form: powder
pka: 2.66±0.50(Predicted)
color: Colorless
λmax: < 300 nm
Biological Applications: Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 26-36
RIDADR: NA 1993 / PGIII
WGK Germany: 3
F: 10-21

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: C1359
Product name: Calcein AM solution
Purity: 4?mM in DMSO, ≥90% (HPLC), solution
Packaging: 100μL
Price: $327
Updated: 2024/03/01

Sigma-Aldrich

Product number: 56496
Product name: Calcein-AM
Purity: Small Package (20 X 50 μg ), ≥96.0% (HPLC)
Packaging: 20x50μg
Price: $905
Updated: 2024/03/01

Sigma-Aldrich

Product number: 17783
Product name: Calcein-AM
Purity: BioReagent, suitable for fluorescence, ≥96.0% (HPLC)
Packaging: 1mg
Price: $568
Updated: 2024/03/01

Sigma-Aldrich

Product number: 17783
Product name: Calcein-AM
Purity: BioReagent, suitable for fluorescence, ≥96.0% (HPLC)
Packaging: 5mg
Price: $2460
Updated: 2024/03/01

Cayman Chemical

Product number: 14948
Product name: Calcein AM
Purity: ≥90%
Packaging: 500μg
Price: $73
Updated: 2024/03/01

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CALCEIN-AM Chemical Properties,Usage,Production

Chemical Properties

A colorless to slightly yellow solid. Soluble in dimethyl sulfoxide.

Uses

Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.

Uses

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.

Uses

A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

Definition

ChEBI: Calcein am is an organic heteropentacyclic compound that is calcein in which all four carboxy group hydrogens have been substituted by (acetyloxy)methoxy groups and the hyrodgens of the two hydroxy groups have been substituted by acetyl groups. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases. It has a role as a fluorochrome. It is an acetate ester, an organic heteropentacyclic compound, a gamma-lactone, an oxaspiro compound, a member of 2-benzofurans and a xanthene dye. It is functionally related to a calcein.

General Description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.

storage

-20°C

References

[1] JAMES HYNES . Fluorescence-Based Cell Viability Screening Assays Using Water-Soluble Oxygen Probes[J]. SLAS Discovery, 2003, 8 3: Pages 264-272. DOI:10.1177/1087057103008003004.
[2] SABNIS R W. Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications[C]. 2010: 0. DOI:10.1002/9780470586242.
[3] Polli, J.W., Wring, S.A., Humphreys, J.E., et al. Rational use of in vitro P-glycoprotein assays in drug discovery. J. Pharmacol. Exp. Ther. 299(2), 620-628 (2001).
[4] Tiberghien, F., and Loor, F. Ranking of P-glycoprotein substates and inhibitors by a calcein-AM fluorometry screening assay. Anticancer Drugs 7(5), 568-578 (1996).
[5] Szakács, G., Jakab, K., Antal, F., et al. Diagnostics of multidrug resistance in cancer. Pathol.Oncol.Res. 4(4), 251-257 (2016).
[6] X M WANG. A new microcellular cytotoxicity test based on calcein AM release.[J]. Human Immunology, 1993, 37 4: 264-270. DOI:10.1016/0198-8859(93)90510-8.
[7] ZSOLT HOLLó . Calcein accumulation as a fluorometric functional assay of the multidrug transporter[J]. Biochimica et biophysica acta. Biomembranes, 1994, 1191 2: Pages 384-388. DOI:10.1016/0005-2736(94)90190-2.

CALCEIN-AM Preparation Products And Raw materials

Raw materials

Preparation Products

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CALCEIN-AM Suppliers

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148504-34-1, CALCEIN-AMRelated Search:

TERT-BUTYL ISOCYANIDE Tris(2,4-pentanedionato)chroMiuM(III) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Benzyl isocyanide Cupric acetylacetonate METHYL ISOCYANOACETATE COBALT(II) ACETYLACETONATE Tosylmethyl isocyanide DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) SALCOMINE 2,4-PENTANEDIONE, SILVER DERIVATIVE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Aluminum acetylacetonate Ethyl isocyanoacetate Ferric acetylacetonate PHENYLSELENOL N-BUTYLISOCYANIDE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)

3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution

Calcein AM solution

CALCEIN, AM;CAL-AM

4',5'-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester

Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester

Calcein AcetoxyMethyl Ester

N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1'-Bis[(acetyloxy) Methyl] Ester

NSC 689290

CALCEIN-ACETOXYMETHYL ESTER, DIACETATE

CALCEIN-AM

CAL-AM

3',6'-DI(O-ACETYL)-2',7'-BIS(N,N-BIS(CARBOXYMETHYL)-AMINOMETHYL)FLUORESCEIN-TETRAACETOXYMETHYL ESTER

Cellstain- Calcein-AM

CALCEIN-AM SOLUTION, 1 MMOL IN DMSO*

CALCEIN AM, 4MM IN ANHYDROUS DMSO

-Cellstain-Calcein-AmSolution

calcein o,o'-diacetate tetrakis(acetoxymethyl)ester

Calcein O,o0-diacetate Tetrakis(acetoxymethyl) ester

Glycine, N,n0-[[30,60-bis(acetyloxy)-3-oxospiro[ isobenzofuran-1(3h),90-[9h]xanthene]-20,70-diyl] bis(methylene)]bis[n-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, bis[(acetyloxy)methyl] Ester

Calcein, AM *CAS 148504-34-1*

Calcein, AM *UltraPure grade* *CAS 148504-34-1*

Calcein AM (Calcein acetoxymethyl ester)

Calcein-AM - CAS 148504-34-1 - Calbiochem

Cellstain Calcein AM Solution(1mM/l DMSO) (3',6'-Di(O-acetyl)-4',5'-bis[N,N-bis(carboxymethy

Calcein-AM (3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceinTetraacetoxymethyl Ester)

Glycine, N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, 1,1'-bis[(acetyloxy)methyl] ester

AM /Calcein-AM

Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylene))bis(azanetriyl))tetraacetate

148504-34-1

C46H46N2O23

Amines

Aromatics

Fluorescent Labels & Indicators

Heterocycles

Intermediates & Fine Chemicals

Pharmaceuticals

Xanthene

CCalcium Signaling Research Tools

Calcium Probes

Fluorescent

Stains and Dyes

Stains&Dyes, A to