ChemicalBook > CAS DataBase List > GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
- Product Name
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
- CAS No.
- 9001-50-7
- Chemical Name
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
- Synonyms
- gapd;G3PD;cb609;GAPDH;G-3-PDH;MGC88685;mg:bb02e05;EC: 1.2.1.12;EC 1.2.1.1.2;GAPDH)/CH8530
- CBNumber
- CB6673129
- Molecular Formula
- C3H7O6P
- Formula Weight
- 170.057841
- MOL File
- 9001-50-7.mol
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GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Property
- storage temp.
- -20°C
- form
- suspension
- color
- white
- biological source
- rabbit muscle
- Specific Activity
- 70-140units/mg protein
- EPA Substance Registry System
- Dehydrogenase, glyceraldehyde phosphate (9001-50-7)
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Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H319Causes serious eye irritation
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P337+P313IF eye irritation persists: Get medical advice/attention.
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N-Bromosuccinimide Price
Sigma-Aldrich
- Product number
- G2267
- Product name
- Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle
- Purity
- lyophilized powder, ≥75?units/mg protein
- Packaging
- 500units
- Price
- $71.8
- Updated
- 2025/07/31
Sigma-Aldrich
- Product number
- SRE0024
- Product name
- Glyceraldehyde-3-phosphate dehydrogenase human
- Purity
- recombinant, expressed in Escherichia coli
- Packaging
- 100μg
- Price
- $478.8
- Updated
- 2025/07/31
Sigma-Aldrich
- Product number
- G2267
- Product name
- Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle
- Purity
- lyophilized powder, ≥75?units/mg protein
- Packaging
- 1000units
- Price
- $106
- Updated
- 2025/07/31
Sigma-Aldrich
- Product number
- G6019
- Product name
- Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes
- Purity
- lyophilized powder, 50-150?units/mg protein
- Packaging
- 100units
- Price
- $475.95
- Updated
- 2025/07/31
Sigma-Aldrich
- Product number
- G5262
- Product name
- GAPDH
- Purity
- standard for protein electrophoresis
- Packaging
- 1vial
- Price
- $89.11
- Updated
- 2025/07/31
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GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Chemical Properties,Usage,Production
Chemical Properties
White crystalline material, suspended in 3.2 mol/L ammonium sulfate solution (containing 0.1 mmol/L EDTA). Stability: under the condition of 5℃, the suspension can be kept for several months with the same activity; changing the new medium regularly can keep the activity unchanged for a long time; freezing or thawing can inactivate it. Each molecule of glyceraldehyde-3-phosphate dehydrogenase contains 3.8-4.0 highly active cysteine residues, and can bind to 4 molecules of coenzyme I (NAD+) or reduced coenzyme I (NADH), the enzyme reaction: D-glyceraldehyde-3-phosphate + coenzyme I + inorganic phosphorus ═1,3-bisphosphate-D-glyceroic acid + reduced coenzyme I.
Uses
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
- for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
- to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
- in anti-aggregation assays
- in purified GAPDH studies
General Description
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.
Biochem/physiol Actions
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>.
Purification Methods
Purify the dehydrogenase from rabbit muscle by extraction with 0.03N KOH and precipitate it with (NH4)2SO4 (0.52 of saturation). The clear supernatant is adjusted to pH 7.5, and NH3 is added dropwise to pH 8.2-8.4. Crystals appear sometimes even without seeding. The crystals are dissolved in H2O, filtered to remove suspended material and 2 volumes of saturated (NH4)2SO4 at pH 8.2-8.4 is added. After 1hour the crystals appear. Recrystallise it in the same way. [Cori et al. J Biol Chem 173 605 1948, Furfine & Velick J Biol Chem 240 844 1965, The Enzymes 7 243 1963, Lui & Huskey Biochemistry 31 6998 1992.] The Km values are: NADH (3.3WM) and 1,3-diphosphoglycerate (8x10-7M) in pH 7.4 imidazole buffer at 26o, NAD (13WM), glyceraldehyde-3-P (90WM), Pi (2.9x10-4M), and arsenate (69WM) in 8.6 M NaHCO3 buffer at 26oC. [Orsi & Cleland Biochemistry 11 102 1972.]
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE manufacturers
Career Henan Chemical Co
- Product
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 9001-50-7
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- 95%-99%
- Supply Ability
- 1000kg
- Release date
- 2018-12-24
9001-50-7, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASERelated Search:
METHYL ISOCYANOACETATE
Tosylmethyl isocyanide
Ferric acetylacetonate
Tris(2,4-pentanedionato)chroMiuM(III)
1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
TERT-BUTYL ISOCYANIDE
DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
COBALT(II) ACETYLACETONATE
PHENYLSELENOL
SALCOMINE
Cupric acetylacetonate
TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)
N-BUTYLISOCYANIDE
Ethyl isocyanoacetate
Benzyl isocyanide
2,4-PENTANEDIONE, SILVER DERIVATIVE
Aluminum acetylacetonate