ChemicalBook > CAS DataBase List > Toluidine Blue O

Toluidine Blue O

Product Name
Toluidine Blue O
CAS No.
92-31-9
Chemical Name
Toluidine Blue O
Synonyms
TB;klot;fklot;menodin;gabilin;tolazul;Blutene;CI 52040;TOLONIUM;Nsc 36758
CBNumber
CB6674575
Molecular Formula
C15H16ClN3S
Formula Weight
305.82
MOL File
92-31-9.mol
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Toluidine Blue O Property

Melting point:
>200℃
storage temp. 
Inert atmosphere,Room Temperature
solubility 
H2O: soluble1mg/mL
form 
Solid
pka
2.4, 11.6(at 25℃)
Colour Index 
52040
color 
Dark green to black
PH
2.8 (H2O, 25℃)
Water Solubility 
3.82 g/100 mL
λmax
626 nm, 630 nm
Merck 
14,9520
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Bound hyaluronan assay; DNA assay; bioelectronic applications; biofuel cells; microbial fuel cells; detecting bacteria,body fluid,nucleic acids,45,proteins,viruses; identifying Gram-negative bacteria,b-amyloid reducing agents
EPA Substance Registry System
Tolonium chloride (92-31-9)
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Safety

Risk Statements 
36/37/38
Safety Statements 
22-24/25
WGK Germany 
3
RTECS 
SP5670000
TSCA 
Yes
HS Code 
32041300
Toxicity
LD50 in mice, rats, rabbits (mg/kg): 27.56, 28.93, 13.44 i.v. (Haley, Storlarsky)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
198161
Product name
Toluidine Blue O
Purity
certified by the Biological Stain Commission
Packaging
5g
Price
$125
Updated
2024/03/01
Sigma-Aldrich
Product number
198161
Product name
Toluidine Blue O
Purity
certified by the Biological Stain Commission
Packaging
25g
Price
$562
Updated
2024/03/01
Sigma-Aldrich
Product number
364-M
Product name
Toluidine Blue O
Purity
HARLECO? For use in histological sections, animal tissue and as a general nuclear stain
Packaging
25G
Price
$293
Updated
2023/01/07
Sigma-Aldrich
Product number
TX0875
Product name
Toluidine Blue O
Purity
Meets Reagent Specifications for testing USP/NF monographs
Packaging
50G
Price
$478
Updated
2022/05/15
Sigma-Aldrich
Product number
1.15930
Product name
Toluidine blue O (C.I.52040)
Purity
formicroscopyCertistain?
Packaging
25g
Price
$259
Updated
2022/05/15
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Toluidine Blue O Chemical Properties,Usage,Production

Chemical Properties

Toluidine Blue O is a dark green or black-gray powder with bronze luster. It is soluble in water (3.82g/100ml), producing a blue-violet solution, slightly soluble in ethanol (0.57g/100ml) to give a blue solution, very slightly soluble in chloroform, and almost insoluble in ether.

Originator

Blutene,Abbott,US,1953

Uses

Toluidine Blue O is a metachromatic dye suitable for a wide variety of histological staining procedures. It is used for direct dyeing and printing of wool and silk, as well as a biological stain and hemostatic agent.

Definition

ChEBI: Toluidine Blue O is an organic chloride salt having 3-amino-7-(dimethylamino)-2-methylphenothiazin-5-ium (tolonium) as the counterion. It is a blue nuclear counterstain that can be used to demonstrate Nissl substance and is also useful for staining mast cell granules, both in metachromatic and orthochromatic techniques. It has a role as a fluorochrome, a histological dye and a diagnostic agent. It contains a tolonium.

Preparation

by heavy chromate salt in the presence of sodium content and deal with N1,N1-dimethylbenzene-1,4-diamine, transforming it into the content and sulfonic acid, this product then through heavy chromate salt and O-Methylaniline processing, into a unique of sulfur acid amine generation, then use heavy chromate salt and copper sulfate closed loop and oxidation, Finally,?the?zinc chloride?salt?precipitation

Manufacturing Process

As taken from US Patent 416,055 (probably the oldest patent on the manufacture of a currently-used drug): In carrying out this process about 6 pbw of dimethyl-p-phenylenediamine was dissolved in about 18 pbw of hydrochloric acid of about 1.16 specific gravity and then a solution of about 3.8 pbw of nitrite of soda in about 6 pbw of water was gradually added. The hydrochlorate of nitroso-dimethylaniline thus produced in the well-known manner is then submitted to the reducing action of zinc-dust by adding, first about 30 pbw of hydrochloric acid of about 1.16 specific gravity and then (in small portions at a time) about 10 pbw of zinc-dust as is well understood by chemists. The solution of hydrochlorate of paramido-dimethylaniline thus obtained is afterwards diluted with about 250 pbw of water and then the uncombined hydrochloric acid contained in the solution is, if any, neutralized by the addition of an alkali. There are then added about 16 pbw of sulfate of alumina and about 13 pbw of thiosulfate of sodium, (hyposulfite of soda) and immediately afterwards a solution of about 5 pbw of bichromate of potash in about 60 pbw of water is quickly run in.
In this stage of the process the formation of an acid sulfureted compound of paramidodimethylaniline takes place, possessing the formula C8H11N2SSO3H(paramido-dimethylaniline-thiosulfonic acid). Without previous separation of this intermediate compound a solution of about 5.3 pbw of orthotoluidine, in the requisite amount of dilute hydrochloric acid (about 6 pbw of hydrochloric acid, SG about 1.16, diluted with about 6 pbw water) and shortly afterwards a solution of about 14 pbw of bichromate of potash in about 160 parts by weight of water is then added under constant agitation, when a precipitate will be formed chiefly consisting of a green indamine possessing in its dry condition the formula C15H17N3S2O3. In order to transform the same into toluidine-blue, about 50 pbw of a solution of chloride of zinc of about 1.5 specific gravity are added and the mixture thus obtained is boiled during about half an hour, when, after cooling, the toluidine-blue thus formed will separate out and may then be filtered and purified, if necessary, by repeated solution in water and precipitation by means of chloride of sodium and chloride of zinc.
In the above described process the sulfate of alumina may be dispensed with and replaced by as much hydrochloric, sulfuric, or acetic acid as will be required to liberate the thiosulfuric acid from the thiosulfate of sodium employed.
Toluidine-blue prepared as above described presents the following characteristic properties: It consists principally of the hydrochlorate of dimethyltoluthionine, the composition of which corresponds to the formula C15H15N3SHCl.

Therapeutic Function

Coagulant

Staining Procedures

The staining solution is prepared by dissolving 0.05% toluidine blue O in buffer, pH 4.4-6.8. Sections or whole tissues are stained for 5-10 min followed by differentiation using the same buffer until excess stain is removed. Cellulose and other glucans become red-violet depending on pH, lignified elements and other polyphenols become bluish-green. Fungal hyphae are often distinguished from the surrounding host tissue because of the difference in cytoplasmic pH.

Properties and Applications

Soluble in water for blue purple, soluble in ethanol to blue. The strong sulfuric acid for yellow green, diluted to blue. The dye solution to join sodium hydroxide have dark purple precipitation.

Standard( Cotton ) Light Fastness Persperation Fastness Ironing Fastness Soaping
Fading Stain Fading Stain Fading Stain
C 2-3 1-2

Toluidine Blue O Preparation Products And Raw materials

Raw materials

Preparation Products

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Toluidine Blue O Suppliers

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View Lastest Price from Toluidine Blue O manufacturers

Hebei Kingfiner Technology Development Co.Ltd
Product
Basic Blue 17 92-31-9
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
500000kg
Release date
2023-06-15
Jiangsu Boquan Biotechnology Co., Ltd.
Product
Basic Blue 17 92-31-9
Price
US $365.00/g
Min. Order
100g
Purity
85%
Supply Ability
100kg
Release date
2023-02-21
Career Henan Chemical Co
Product
Basic Blue 17 92-31-9
Price
US $1.00/KG
Min. Order
1KG
Purity
98%HPLC
Supply Ability
10t
Release date
2019-12-23

92-31-9, Toluidine Blue O Related Search:

Benzyl chloride Calcium chloride Choline chloride Mepiquat chloride Buprofezin Polyvinyl chloride 2-[2-HYDROXY-3-(2,4-XYLYLCARBAMOYL)-1-NAPHTHYLAZO]PHENOL Hydrochlorothiazide Alkaline Phosphatase p-Toluidine Ammonium chloride o-Toluidine Basic Blue 7 Trypan Blue Cupric carbonate basic Thiamethoxam m-Toluidine Sodium chloride

  • DIMETHYLTOLUTHIONINE CHLORIDE
  • CI NO 52040
  • CI BASIC BLUE 17
  • CI 52040
  • fklot
  • gabilin
  • klot
  • menodin
  • modrtoluidinova
  • modrzasadita17
  • Phenothiazin-5-ium,3-amino-7-(dimethylamino)-2-methyl-,chloride
  • schultz1041
  • CARBOL TOLUIDINE BLUE
  • BASIC BLUE
  • BASIC BLUE 17
  • BLUTENE CHLORIDE
  • METHYLENE BLUE T EXTRA
  • METHYLENE BLUE T
  • METHYLENE BLUE T50
  • METHYLENE BLUE T50 OR T EXTRA
  • 3-AMINO-7-(DIMETHYLAMINO)-2-METHYLPHENAZATHIONIUM CHLORIDE
  • TB
  • TOLONIUM CHLORIDE
  • TOLUIDINE BLUE O
  • TOLUIDINE BLUE
  • TOLUIDINE BLUE 0
  • 3-amino-7-(dimethylamino)-2-methyl-phenothiazin-5-iuchloride
  • 3-Amino-7-(dimethylamino)-2-methylphenothiazin-5-iumchloride
  • TOLUIDINE BLUE O CERTIFIED
  • ToluidineBlue(BasicBlue)
  • ToludineBlue
  • Butene chloride
  • Toluidine Blue O, pure
  • tolazul
  • toluidinblau
  • toluidineblue(phenothiazinedye)
  • toluidineblue0(basicblue17)
  • toluidineblueoo
  • TOLUIDINEBLUECHLORIDE
  • TOLONIUM
  • Toluidine Blue O, Biological stain
  • TOLUIDINE BLUE O, CERTIFICATED
  • Tolonium chloride (C.I. 52040)
  • Toluidine blue (C.I. 52040)
  • Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
  • TOLUIDINE BLUE O SALTRESEARCH GRADE
  • Toluidine blue, C.I. 52040, for microscopy
  • ToluniuM chloride
  • Toluidine Blue O, pure 25GR
  • Toluidine Blue O, pure 5GR
  • TOLUIDINE BLUE O (C.I.52040) FOR MICROSC
  • POTASSIUM FERRICYANIDE ACS GRADE
  • Toluidine blue O (C.I.52040) for microscopy Certistain
  • Blutene
  • Nsc 36758
  • O-toluidine Blue
  • 0.05%Toluidine Blue Solution(pH7.0)
  • oloniumchloride