Tetramethylbenzidine

ChemicalBook > CAS DataBase List > Tetramethylbenzidine

Tetramethylbenzidine

Product Name: Tetramethylbenzidine
CAS No.: 54827-17-7
Chemical Name: Tetramethylbenzidine
Synonyms: TMB;Tmbz;TMB SUBSTRATE;Tetramethylbenzidine (TMB);3,3,5,5-TETRAMETHYLBENZIDINE, STANDARD F OR GC;4-(4-Amino-3,5-dimethylphenyl)-2,6-dimethylaniline;TMB-E;TMB-B;TMB/H;TMB/M
CBNumber: CB6682185
Molecular Formula: C16H20N2
Formula Weight: 240.34
MOL File: 54827-17-7.mol

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Tetramethylbenzidine Property

Melting point:: 168-171 °C(lit.)
Boiling point:: 100 °C
Density: 1
refractive index: 1.5519 (estimate)
storage temp.: 2-8°C
solubility: Slightly soluble. <0.1 g/100 mL at 20°C.
pka: 4.49±0.10(Predicted)
form: tablet
color: Cream
Water Solubility: Slightly soluble. <0.1 g/100 mL at 20 ºC
Sensitive: Light Sensitive
BRN: 2808541
Stability:: Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
CAS DataBase Reference: 54827-17-7(CAS DataBase Reference)
EPA Substance Registry System: 3,3',5,5'-Tetramethylbenzidine (54827-17-7)

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Safety

Hazard Codes: Xi,Xn
Risk Statements: 36/38-36/37/38-22-41
Safety Statements: 26-36-36/37-39
RIDADR: UN 2796 8/PG 2
WGK Germany: 3
RTECS: DV2300000
F: 8-10
TSCA: Yes
HazardClass: 6.1
HS Code: 29215990
Hazardous Substances Data: 54827-17-7(Hazardous Substances Data)

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H225Highly Flammable liquid and vapour

H302Harmful if swallowed

H312Harmful in contact with skin

H315Causes skin irritation

H319Causes serious eye irritation

H332Harmful if inhaled

H335May cause respiratory irritation

H370Causes damage to organs

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: T5525
Product name: 3,3′,5,5′-Tetramethylbenzidine
Purity: tablet, 1 mg substrate per tablet
Packaging: 50tablets
Price: $223
Updated: 2024/03/01

Sigma-Aldrich

Product number: T5525
Product name: 3,3′,5,5′-Tetramethylbenzidine
Purity: tablet, 1 mg substrate per tablet
Packaging: 100tablets
Price: $381
Updated: 2024/03/01

Sigma-Aldrich

Product number: 11484281001
Product name: BM Blue POD Substrate, soluble
Purity: 3,3′-5,5′-Tetramethylbenzidine (TMB)
Packaging: 100mL
Price: $146
Updated: 2024/03/01

Sigma-Aldrich

Product number: 1.08622
Product name: 3,3′,5,5′-Tetramethylbenzidine
Purity: GR for analysis
Packaging: 1g
Price: $145
Updated: 2024/03/01

Sigma-Aldrich

Product number: 09743
Product name: 3,3′,5,5′-Tetramethylbenzidine
Purity: standard for GC
Packaging: 250mg
Price: $36.9
Updated: 2024/03/01

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Tetramethylbenzidine Chemical Properties,Usage,Production

Description

Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.

Chemical Properties

Tetramethylbenzidine is a white or light yellow solid, odorless, tasteless, insoluble in water, easily soluble in acetone, ether, dimethyl sulfoxide, dimethylformamide and other organic solvents. It forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights.

Uses

3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.

Preparation

The synthesis of 3,3',5,5'-Tetramethylbenzidine involved using 2,6-dimethylaniline as the raw material. The process included activation, oxidative coupling, and purification, resulting in the production of pure material. The yield of 3,3,5,5-tetramethylbenzidine was 65%.

Reactions

TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.

Shows the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethylbenzidine diimine
The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at the wavelengths of 370 and 650 nm.

General Description

3,3',5,5'-tetramethylbenzidine appears as pale yellow crystals or off-white powder. (NTP, 1992)

Air & Water Reactions

Insoluble in water.

Reactivity Profile

Tetramethylbenzidine is sensitive to prolonged exposure to light . Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.

Fire Hazard

Flash point data for Tetramethylbenzidine are not available, however, Tetramethylbenzidine is probably combustible.

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in?situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Carcinogenicity

The carcinogenic potential of Tetramethylbenzidine is uncertain due to conflicting evidence. It is not mutagenic by the Ames test, and did not induce formation of tumors in a single-arm study of 24 rats. On that evidence, it has been used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.
DOI: 10.1177/26.2.24068

Tetramethylbenzidine Preparation Products And Raw materials

Raw materials

ZINC Sodium dithionite potassium ferricyanide Ethanol Palladium Polyphosphoric acid Copper(II) sulfate pentahydrate Diethyl ether Ammonium chloride Hydrogen Dichloromethane Potassium permanganate Acetic acid Methanol 2,6-Dimethylaniline Sulfuric acid

Preparation Products

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View Lastest Price from Tetramethylbenzidine manufacturers

Ouhuang Engineering Materials (Hubei) Co., Ltd

Product: Tetramethylbenzidine 54827-17-7
Price: US $50.00/kg
Min. Order: 1kg
Purity: 99.10%
Supply Ability: 5000kg
Release date: 2024-04-17

airuikechemical co., ltd.

Product: tetramethylbenzidine 54827-17-7
Price: US $0.00-0.00/Kg
Min. Order: 1Kg
Purity: 99.9%
Supply Ability: 200tons
Release date: 2024-04-10

Shanghai Chinqesen Biotechnology Co., Ltd.

Product: Tetramethylbenzidine 54827-17-7
Price: US $30.00-20.00/BOX
Min. Order: 10BOX
Purity: 99
Supply Ability: 20TONS
Release date: 2023-11-07

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