ChemicalBook > CAS DataBase List > Tetramethylbenzidine

Tetramethylbenzidine

Product Name
Tetramethylbenzidine
CAS No.
54827-17-7
Chemical Name
Tetramethylbenzidine
Synonyms
TMB;Tmbz;TMB SUBSTRATE;Tetramethylbenzidine (TMB);3,3,5,5-TETRAMETHYLBENZIDINE, STANDARD F OR GC;4-(4-Amino-3,5-dimethylphenyl)-2,6-dimethylaniline;TMB-E;TMB-B;TMB/H;TMB/M
CBNumber
CB6682185
Molecular Formula
C16H20N2
Formula Weight
240.34
MOL File
54827-17-7.mol
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Tetramethylbenzidine Property

Melting point:
168-171 °C(lit.)
Boiling point:
100 °C
Density 
1
refractive index 
1.5519 (estimate)
storage temp. 
2-8°C
solubility 
Slightly soluble. <0.1 g/100 mL at 20°C.
pka
4.49±0.10(Predicted)
form 
tablet
color 
Cream
Water Solubility 
Slightly soluble. <0.1 g/100 mL at 20 ºC
Sensitive 
Light Sensitive
BRN 
2808541
Stability:
Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
CAS DataBase Reference
54827-17-7(CAS DataBase Reference)
EPA Substance Registry System
3,3',5,5'-Tetramethylbenzidine (54827-17-7)
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Safety

Hazard Codes 
Xi,Xn
Risk Statements 
36/38-36/37/38-22-41
Safety Statements 
26-36-36/37-39
RIDADR 
UN 2796 8/PG 2
WGK Germany 
3
RTECS 
DV2300000
F 
8-10
TSCA 
Yes
HazardClass 
6.1
HS Code 
29215990
Hazardous Substances Data
54827-17-7(Hazardous Substances Data)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H225Highly Flammable liquid and vapour

H302Harmful if swallowed

H312Harmful in contact with skin

H315Causes skin irritation

H319Causes serious eye irritation

H332Harmful if inhaled

H335May cause respiratory irritation

H370Causes damage to organs

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
T5525
Product name
3,3′,5,5′-Tetramethylbenzidine
Purity
tablet, 1 mg substrate per tablet
Packaging
50tablets
Price
$223
Updated
2024/03/01
Sigma-Aldrich
Product number
T5525
Product name
3,3′,5,5′-Tetramethylbenzidine
Purity
tablet, 1 mg substrate per tablet
Packaging
100tablets
Price
$381
Updated
2024/03/01
Sigma-Aldrich
Product number
11484281001
Product name
BM Blue POD Substrate, soluble
Purity
3,3′-5,5′-Tetramethylbenzidine (TMB)
Packaging
100mL
Price
$146
Updated
2024/03/01
Sigma-Aldrich
Product number
1.08622
Product name
3,3′,5,5′-Tetramethylbenzidine
Purity
GR for analysis
Packaging
1g
Price
$145
Updated
2024/03/01
Sigma-Aldrich
Product number
09743
Product name
3,3′,5,5′-Tetramethylbenzidine
Purity
standard for GC
Packaging
250mg
Price
$36.9
Updated
2024/03/01
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Tetramethylbenzidine Chemical Properties,Usage,Production

Description

Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.

Chemical Properties

Tetramethylbenzidine is a white or light yellow solid, odorless, tasteless, insoluble in water, easily soluble in acetone, ether, dimethyl sulfoxide, dimethylformamide and other organic solvents. It forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights.

Uses

3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.

Preparation

The synthesis of 3,3',5,5'-Tetramethylbenzidine involved using 2,6-dimethylaniline as the raw material. The process included activation, oxidative coupling, and purification, resulting in the production of pure material. The yield of 3,3,5,5-tetramethylbenzidine was 65%.

Reactions

TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase.

Shows the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethylbenzidine diimine
The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at the wavelengths of 370 and 650 nm.

General Description

3,3',5,5'-tetramethylbenzidine appears as pale yellow crystals or off-white powder. (NTP, 1992)

Air & Water Reactions

Insoluble in water.

Reactivity Profile

Tetramethylbenzidine is sensitive to prolonged exposure to light . Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.

Fire Hazard

Flash point data for Tetramethylbenzidine are not available, however, Tetramethylbenzidine is probably combustible.

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in?situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Carcinogenicity

The carcinogenic potential of Tetramethylbenzidine is uncertain due to conflicting evidence. It is not mutagenic by the Ames test, and did not induce formation of tumors in a single-arm study of 24 rats. On that evidence, it has been used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.
DOI: 10.1177/26.2.24068

Tetramethylbenzidine Preparation Products And Raw materials

Raw materials

ZINC Sodium dithionite potassium ferricyanide Ethanol Palladium Polyphosphoric acid Copper(II) sulfate pentahydrate Diethyl ether Ammonium chloride Hydrogen Dichloromethane Potassium permanganate Acetic acid Methanol 2,6-Dimethylaniline Sulfuric acid

Preparation Products

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View Lastest Price from Tetramethylbenzidine manufacturers

HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
Product
Tetramethylbenzidine 54827-17-7
Price
US $999.00-666.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000
Release date
2024-08-20
Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Tetramethylbenzidine 54827-17-7
Price
US $62.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000kg/week
Release date
2024-05-24
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Tetramethylbenzidine 54827-17-7
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
3500kg/month
Release date
2021-09-10

54827-17-7, TetramethylbenzidineRelated Search:

Octamethylcyclotetrasiloxane 2,2'-Benzidinedisulfonic acid O-Tolidine Tetramethylammonium hydroxide 3,3'-Dichlorobenzidine Benzidine N,N,N',N'-Tetramethylethylenediamine Pigment Yellow 12 Tetramethylguanidine Tetramethylpyrazine Tetramethylene sulfoxide Hexamethylenetetramine 3,3',5,5'-Tetramethylbenzidine dihydrochloride Chlorophacinone N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine sodium salt 3,3'',5,5-TETRAMETHYLBENZIDINESULFATE(TMB-S) N,N,N',N'-Tetramethylbenzidine, 97.50%,N,N,N',N'-TETRAMETHYLBENZIDINE TMB 124

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  • ELISAHRP
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