ChemicalBook > CAS DataBase List > Solvent Black 3

Solvent Black 3

Product Name
Solvent Black 3
CAS No.
4197-25-5
Chemical Name
Solvent Black 3
Synonyms
CI 26150;Nsc 11239;Black 2B;sot-black6;BSudan Bla;Sudanblack;CI NO 26150;typogenblack;FAT BLACK HB;Oil Black Hz
CBNumber
CB7198632
Molecular Formula
C29H24N6
Formula Weight
456.54
MOL File
4197-25-5.mol
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Solvent Black 3 Property

Melting point:
120-124 °C(lit.)
Boiling point:
552.68°C (rough estimate)
Density 
1.4899 (rough estimate)
refractive index 
1.4570 (estimate)
storage temp. 
Store at RT.
solubility 
Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol
form 
Powder/Solid
Colour Index 
26150
pka
2.94±0.40(Predicted)
color 
Very dark brown to black
Water Solubility 
Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water.
λmax
598 nm, 415 nm
Merck 
13,8970
BRN 
723248
Stability:
Light Sensitive
Biological Applications
Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening
CAS DataBase Reference
4197-25-5(CAS DataBase Reference)
EPA Substance Registry System
C.I. Solvent Black 3 (4197-25-5)
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Safety

Hazard Codes 
F,Xi
Risk Statements 
11-36/37/38
Safety Statements 
24/25-16-37/39-26
WGK Germany 
3
RTECS 
SD4431500
TSCA 
Yes
HazardClass 
IRRITANT
HS Code 
32041900
Toxicity
LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
199664
Product name
Sudan Black B
Purity
certified by the Biological Stain Commission
Packaging
25g
Price
$96.2
Updated
2024/03/01
TCI Chemical
Product number
S0113
Product name
Sudan Black B
Packaging
25g
Price
$50
Updated
2024/03/01
Alfa Aesar
Product number
J62268
Product name
Sudan Black B
Packaging
10g
Price
$36.5
Updated
2024/03/01
Alfa Aesar
Product number
J62268
Product name
Sudan Black B
Packaging
25g
Price
$69.6
Updated
2024/03/01
Alfa Aesar
Product number
J62268
Product name
Sudan Black B
Packaging
100g
Price
$127
Updated
2024/03/01
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Solvent Black 3 Chemical Properties,Usage,Production

Chemical Properties

It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added.

Uses

Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.

Definition

ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines.

Preparation

aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling.

Safety Profile

A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.

Staining Procedures

1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids.

Properties and Applications

Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.

Standard Light Fastness Heat-resistant(℃) water Sodium Carbonate(5%) Hydrochloric acid(5%)
Melting point Stable
ISO General 126 100 Insoluble No change No change

Solvent Black 3 Preparation Products And Raw materials

Raw materials

Preparation Products

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Solvent Black 3 Suppliers

Hubei Guangao Biotechnology Co., Ltd
Tel
027-027-59223056 18162699093
Fax
027-59223056
Email
1208480011@qq.com
Country
China
ProdList
9924
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
TAIYUAN RHF CO.,LTD.
Tel
+86 351 7031519
Fax
+86 351 7031519
Email
sales@RHFChem.com
Country
China
ProdList
2338
Advantage
56
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
Pure Chemistry Scientific Inc.
Tel
001-857-928-2050 or 1-888-588-9418
Fax
001-617-206-9595
Email
sales@chemreagents.com
Country
United States
ProdList
10186
Advantage
62
Winchem Industrial Co. Ltd
Tel
0574-83851061
Fax
86-574-87083209
Email
info@win-chemical.com
Country
China
ProdList
500
Advantage
55
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
Sichuan Kulinan Technology Co., Ltd
Tel
400-1166-196 18981987031
Fax
028-84555506 800101999
Email
cdhxsj@163.com
Country
China
ProdList
11707
Advantage
57
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View Lastest Price from Solvent Black 3 manufacturers

Shaanxi Dideu Medichem Co. Ltd
Product
SolventBlack3 4197-25-5
Price
US $1.00-50.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
200000KG
Release date
2024-07-31
Winchem industrial co limited
Product
SOLVENT BLACK 3 4197-25-5
Price
US $0.00/kg
Min. Order
25kg
Purity
0.99
Supply Ability
20MT
Release date
2022-12-20
Zhuozhou Wenxi import and Export Co., Ltd
Product
Solvent Black 3 4197-25-5
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-10

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