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AC-DEVD-AFC

Product Name
AC-DEVD-AFC
CAS No.
201608-14-2
Chemical Name
AC-DEVD-AFC
Synonyms
AC-DEVD-AFC;AC-DEVD-AFC?, >98%;AC-DEVD-AFC USP/EP/BP;AC-ASP-GLU-VAL-ASP-AFC;CASPASE-3 SUBSTRATE (AFC);M.W. 729.62 C30H34F3N5O13;CASPASE-3 FLUOROGENIC SUBSTRATE;CASPASE-3 SUBSTRATE VII, FLUOROGENIC;FLUOROGENIC CASPASE 3 SUBSTRATE AC-DEVD-AFC;CASPASE 3 (APOPAIN) SUBSTRATE 1F, FLUOROGENIC
CBNumber
CB7205880
Molecular Formula
C30H34F3N5O13
Formula Weight
729.61
MOL File
201608-14-2.mol
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AC-DEVD-AFC Property

Boiling point:
1152.7±65.0 °C(Predicted)
Density 
1.495±0.06 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
H2O: 1 mg/mL
form 
powder
pka
4.09±0.10(Predicted)
color 
white
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
A0466
Product name
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
Purity
≥90% (HPLC), powder
Packaging
1mg
Price
$203
Updated
2024/03/01
Alfa Aesar
Product number
J64134
Product name
N-Acetyl-Asp-Glu-Val-Asp-7-amino-4-(trifluoromethyl)coumarin
Packaging
5mg
Price
$350
Updated
2023/06/20
Cayman Chemical
Product number
14459
Product name
Ac-DEVD-AFC
Purity
≥98%
Packaging
1mg
Price
$50
Updated
2024/03/01
Cayman Chemical
Product number
14459
Product name
Ac-DEVD-AFC
Purity
≥98%
Packaging
5mg
Price
$184
Updated
2024/03/01
Cayman Chemical
Product number
14459
Product name
Ac-DEVD-AFC
Purity
≥98%
Packaging
10mg
Price
$341
Updated
2024/03/01
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AC-DEVD-AFC Chemical Properties,Usage,Production

Uses

Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes'' shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.

Biological Activity

ac-devd-afc is a fluorogenic substrate for activated caspase-3 (cpp32) with km value of 9.7 μm, and related cysteine proteases [1][2].apoptosis is a process of programmed cell death that occurs in multicellular organisms. caspase are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation. caspase activation is a major event in apoptosis. caspase-3 cleaves and activates caspases 6 and 7, and is processed and activated by caspases 8, 9, and 10 [1][2][3].ac-devd-afc (n-acetyl-asp-glu-val-asp-7-amido-4-trifluoromethylcoumarin) is a fluorogenic substrate for activated caspase-3 and related caspases. during apoptosis, activated caspase-3 cleaves poly (adp-ribose) polymerase, which it specifically targets at the amino sequence asp-glu-val-asp (devd). sequence is based on parp cleavage at asp216 for caspase-3 [1][2]. caspase activity can be quantified by fluorescent detection of free afc (7-amino-4-trifluoromethylcoumarin) at excitation 400 nm and emission 505 nm [2].

Biochem/physiol Actions

A fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. The 7-amido-4-trifluoromethylcoumarin derivative has better membrane permeability than the 7-amido-4-methylcoumarin derivative (A1086).

References

[1]. lazebnik ya1, kaufmann sh, desnoyers s, et al. cleavage of poly(adp-ribose) polymerase by a proteinase with properties like ice. nature. 1994 sep 22;371(6495):346-7.
[2]. xiang, j.,chao, d.t., and korsmeyer, s.j. bax-induced cell death may not require interleukin 1β-converting enzyme-like proteases. proceedings of the national academy of sciences of the united states of america 93, 14559-14563 (1996).

AC-DEVD-AFC Preparation Products And Raw materials

Raw materials

Preparation Products

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AC-DEVD-AFC Suppliers

China 99 France 1 Germany 3 Japan 2 Switzerland 2 United Kingdom 4 United States 19 Global 130
Nacalai Tesque, Inc.
Tel
--
Fax
--
Email
info-tech@nacalai.co.jp
Country
Japan
ProdList
6046
Advantage
75
Kanto Chemical Co., Inc.
Tel
--
Fax
--
Email
reag-info@gms.kanto.co.jp
Country
Japan
ProdList
6756
Advantage
74

201608-14-2, AC-DEVD-AFCRelated Search:

AC-VDVAD-PNA Z-VAD-FMK AC-YVAD-PNA CASPASE INHIBITOR3 AC-VEID-AMC Z-ASP-2,6-DICHLOROBENZOYLOXYMETHYLKETONE Z-YVAD-AFC ALPHA-(TRICHLOROMETHYL)-4-PYRIDINEETHANOL AC-VDVAD-CHO PAC1 AC-LEHD-AFC AC-LEU-GLU-THR-ASP-AFC AC-DEVD-CHO N-acetyl aspartyl-glutaMic acid AC-DEVD-AFC 4-(3-METHOXYANILINO)-4-OXOBUTANOIC ACID SUC-ALA-ALA-ALA-AMC AC-DEVD-AMC

  • N-ACETYL-ASP-GLU-VAL-ASP 7-AMIDO-4-TRIFLUOROMETHYLCOUMARIN
  • N-ACETYL-ASP-GLU-VAL-ASP-(7-AMINO-4-TRIFLUOROMETHYLCOUMARIN)
  • N-ACETYL-ASP-GLU-VAL-ASP-7-AMINO-4-TRIFLUOROMETHYLCOUMARIN AFC
  • CASPASE 3 (APOPAIN) SUBSTRATE 1F, FLUOROGENIC
  • CASPASE-3 SUBSTRATE (AFC)
  • CASPASE-3 SUBSTRATE VII, FLUOROGENIC
  • CASPASE-3 FLUOROGENIC SUBSTRATE
  • FLUOROGENIC CASPASE 3 SUBSTRATE AC-DEVD-AFC
  • AC-ASP-GLU-VAL-ASP-7-AMINO-4-TRIFLUORO-METHYLCOUMARIN
  • Ac-DEVD-AFC,N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin, >98%
  • AC-DEVD-AFC?, >98%
  • AC-ASP-GLU-VAL-ASP-AFC
  • AC-DEVD-AFC
  • M.W. 729.62 C30H34F3N5O13
  • Ac-Asp-Glu-Val-Asp -7-amino-4-trrifluoromethylcoumarin
  • L-α-Asparagine, N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-
  • AC-DEVD-AFC USP/EP/BP
  • Ac-DEVD-AFC，N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
  • 201608-14-2
  • C30H34N5O13F3
  • C30H34F3N5O13
  • Pepetides
  • peptide
  • Caspases/Apoptosis