Z-VAD-FMK

Product Name: Z-VAD-FMK
CAS No.: 187389-52-2
Chemical Name: Z-VAD-FMK
Synonyms: CS-2005;CS-1507;Z-VAD-FMK;HGNC:1504;Z-VAD(OME)-FMK;Z-VA(OME)D-FMK;Z-VADC(OME)-FMK;Z-VAD-FMK, >=98%;Z-VAL-ALA-ASP-FMK;CASPASE INHIBITOR I
CBNumber: CB0203438
Molecular Formula: C22H30FN3O7
Formula Weight: 467.49
MOL File: Mol file

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Z-VAD-FMK Property

Melting point:: >190°C (dec.)
Boiling point:: 732.4±60.0 °C(Predicted)
Density: 1.214±0.06 g/cm3 (20 ºC 760 Torr)
Flash point:: 396.7℃
storage temp.: -20°C
solubility: DMSO: 20 mg/mL
form: powder
pka: 11.05±0.46(Predicted)
color: white
biological source: synthetic
Sequence: Z-Val-Ala-Asp(OMe)-FMK
Stability:: Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week.
InChIKey: MIFGOLAMNLSLGH-QOKNQOGYSA-N
SMILES: C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(OC)=O)C(=O)CF
CAS DataBase Reference: 187389-52-2

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Safety

Safety Statements: 22-24/25
WGK Germany: 3
HS Code: 29145090

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: V116
Product name: Z-VAD-FMK
Purity: solid
Packaging: 2mg
Price: $837
Updated: 2025/07/31

Sigma-Aldrich

Product number: SAB2501669
Product name: Anti-CASP3 antibody produced in goat
Purity: affinity isolated antibody, buffered aqueous solution
Packaging: 100μg
Price: $586
Updated: 2025/07/31

Sigma-Aldrich

Product number: 627610
Product name: Caspase Inhibitor I
Packaging: 1mg
Price: $327
Updated: 2025/07/31

Sigma-Aldrich

Product number: 627610
Product name: Caspase Inhibitor I
Packaging: 5mg
Price: $681
Updated: 2025/07/31

Cayman Chemical

Product number: 14463
Product name: Z-VAD(OMe)-FMK
Purity: ≥95%
Packaging: 1mg
Price: $100
Updated: 2024/03/01

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Z-VAD-FMK Chemical Properties,Usage,Production

Description

Z-VAD-FMK (187389-52-2), cell-permeable, methyl ester form. Potent, irreversible pan-caspase inhibitor. Inhibits caspase activity and apoptosis induction in a variety of cell types (IC50 = 1.5 μM). Active in vivo.

Uses

Z-VAD(OMe)-FMK is a cell-permeable, competitive, and irreversible inhibitor of all caspases. Through this action, it inhibits cleavage of poly(ADP-ribose) polymerase, preventing apoptosis when used at 10-50 μM. It also blocks caspase-mediated apoptosis in vivo. Z-VAD(OMe)-FMK effectively prevents caspase action in inflammasomes.[Cayman Chemical]

Uses

Z-VAD-FMK is a caspase inhibitor that inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentrations of z-FA-CMK.

Definition

ChEBI: Z-Val-Ala-Asp(OMe)-CH2F is a tripeptide consisting of Z-Val-Ala-Asp(OMe) in which the C-terminal OH group has been replaced by a fluoromethyl group. An irreversible pan-caspase inhibitor. It has a role as an apoptosis inhibitor and a protease inhibitor. It is a carbamate ester, a tripeptide and an organofluorine compound.

General Description

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Biological Activity

Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC 50 = 0.0015-5.8 mM). Active in vivo .

Biochem/physiol Actions

Cell permeable: yes

Enzyme inhibitor

This tripeptide halomethyl ketone (FWfree-acid = 467.49 g/mol; Soluble to 9.35 mg/ml in DMSO; CAS RegistryNumber = 187389-52-2), also known as Z-VAD-FMK and caspase inhibitor VI, is a broad-spectrum caspase inhibitor that blocks caspase-mediated apoptosis. Z-VAD-FMK inhibits caspase processing (IC50 = 0.0015–5.8 mM, depending on enzyme and cell type). The aspartate methyl ester derivative (FW = 467.49 g/mol), also known as caspase inhibitor I, is far more cell-permeable. Target (s) : caspases; caspase-1; caspase-2; caspase-3; caspase-4 ; caspase 5; caspase-6; caspase-7; caspase-8 ; caspase-9; caspase-10.

storage

-20°C

References

[1] E A SLEE. Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32.[J]. Biochemical Journal, 1996, 315 ( Pt 1): 21-24. DOI:10.1042/bj3150021
[2] GERALD KÜNSTLE . ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-α[J]. Immunology letters, 1997, 55 1: Pages 5-10. DOI:10.1016/s0165-2478(96)02642-9
[3] M GARCIA-CALVO. Inhibition of human caspases by peptide-based and macromolecular inhibitors.[J]. The Journal of Biological Chemistry, 1998, 273 49: 32608-32613. DOI:10.1074/jbc.273.49.32608

Z-VAD-FMK Preparation Products And Raw materials

Raw materials

Preparation Products

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Z-VAD-FMK Suppliers

China 266 India 2 Japan 1 Switzerland 3 United Kingdom 4 United States 39 Global 315

Shanghai Zhengyi Pharmaceutical Co., Ltd

Tel: 17740884366
Email: derek1229@163.com
Country: China
ProdList: 61
Advantage: 58

JIANGSU CURTIUS BIOTECH CO., LTD

Tel: 0513-89078808 13764678260
Fax: 0513-81001007
Email: 1582941412@qq.com
Country: China
ProdList: 18
Advantage: 58

Hangzhou Peptidego Biotech Co.,Ltd.

Tel: 0571-87213919
Email: Eric@peptidego.com
Country: China
ProdList: 7408
Advantage: 58

Shanghai Fine Biotech Co.，Ltd

Tel: 18221172427
Email: sale@fine-biotech.com
Country: China
ProdList: 2772
Advantage: 58

Changzhou Chenhong Biotechnology Co., Ltd.

Tel: +86-0519-85788828 +86-13775037613
Email: sales@chemrenpharm.com
Country: China
ProdList: 3934
Advantage: 58

Shanghai Zhuyan Biotechnology Co., Ltd.

Tel: 17521259211
Email: 17521259211@163.com
Country: China
ProdList: 135
Advantage: 58

Shanghai Boyle Chemical Co., Ltd.

Tel
Fax: 86-21-57758967
Email: sales@boylechem.com
Country: China
ProdList: 2922
Advantage: 55

3B Pharmachem (Wuhan) International Co.,Ltd.

Tel: 821-50328103-801 18930552037
Fax: 86-21-50328109
Email: 3bsc@sina.com
Country: China
ProdList: 15838
Advantage: 69

Chembest Research Laboratories Limited

Tel: +86-21-20908456
Fax: 021-58180499
Email: sales@BioChemBest.com
Country: China
ProdList: 6003
Advantage: 61

Beijing HwrkChemical Technology Co., Ltd

Tel: 18515581800 18501085097
Fax: 010-89508210
Email: sales.bj@hwrkchemical.com
Country: China
ProdList: 9400
Advantage: 55

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View Lastest Price from Z-VAD-FMK manufacturers

Henan Aochuang Chemical Co.,Ltd.

Product: Z-VAD-FMK 187389-52-2
Price: US $0.00-0.00/KG
Min. Order: 1KG
Purity: 98%
Supply Ability: 1ton
Release date: 2022-10-08

Career Henan Chemical Co

Product: Z-VAD-FMK 187389-52-2
Price: US $1.00/KG
Min. Order: 1G
Purity: 98%
Supply Ability: 100KG
Release date: 2018-08-21

187389-52-2, Z-VAD-FMKRelated Search:

Z-FA-FMK Z-DEVD-FMK Z-Vad-fmk, non-methylated 3-METHYLADENINE MK-2206 2HCl Necrostatin-1 MG-132 Z-VAL-ALA-OH (2S)-2-[(2S)-2-{[(benzyloxy)carbonyl]amino}-3-methylbutanamido]-3-hydroxypropanoic acid PAC1 AC-DEVD-CHO AC-VDVAD-PNA Z-PHE-DL-ALA-FLUOROMETHYLKETONE Z-VAL-TRP-OH CBZ-L-VALINE METHYL ESTER Z-VAL-VAL-ARG-AMC Z-VAL-VAL-OH LY 294002 Hydrochloride

Z-VADC(OME)-FMK

methyl (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-phenylmethoxycarbonylamino-butanoyl]amino]propanoyl]amino]-4-oxo-pentanoate

N-BENZYLOXYCARBONYL-VAL-ALA-ASP FLUOROMETHYL KETONE

Z-VAD-FLUOROMETHYLKETONE

Z-VAD-FMK

Z-VAD-FMK, METHYL ESTER

Z-VAD(OME)-FLUOROMETHYLKETONE

L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-

N-[(Phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-L-alaninamide z-vad -fmk

Anti-CASP3 antibody produced in goat

caspase 3, apoptosis-related cysteine peptidase

caspase 3, apoptosis-related cysteine protease

HGNC:1504

Z-VAD-FMK, >=98%

Z-VAD-FMK, 98%, an irreversible pan-caspase inhibitor

Z-VAD(OME)-FMK;Z-VAL-ALA-ASP(OME)-FMK

Z-VAD(OME)-FMK

Z-VAL-ALA-ASP-FLUOROMETHYLKETONE

Z-VAL-ALA-ASP-FMK

Z-VAL-ALA-ASP(OCH3)-FLUOROMETHYLKETONE

Z-VAL-ALA-ASP(OME)-CH2F

Z-VAL-ALA-ASP(OME)-FLUOROMETHYLKETONE

Z-VAL-ALA-ASP-(OME)-FMK

Z-VAL-ALA-DL-ASP-FLUOROMETHYLKETONE

Z-VAL-ALA-DL-ASP(OME)-FLUOROMETHYLKETONE

Z-VA(OME)D-FMK

N-Benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone

BENZYLOXYCARBONYL-VAL-ALA-ASP(OME)-FLUOROMETHYLKETONE

CARBOBENZOXY-VALYL-ALANYL-ASPARTYL-[O-METHYL]-FLUOROMETHYLKETONE

CASPASE-1, INHIBITOR

CASPASE-1 INHIBITOR VI (FLUOROMETHYLKETONE)

CASPASE INHIBITOR I

CASPASE INHIBITOR VI

Z-VAD-FMK, Non-methylated

PN: WO2005039629 PAGE: 18 claimed protein

Z-VAD-FMK (Cell perMeable)

3: PN: WO0158526 SEQID: 3 claimed sequence

N-[(Phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-L-alaninamide

Z-VAL-ALA-DL-ASP(OME)-FLUOROMETHYLKETONEZ-Val-Ala-DL-Asp-fluoroMethylketone

CS-2005

CS-1507

Z-VAD-FMK (Z-Val-Ala-Asp(OMe)-FMK)

Methyl (S)-3-[(S)-2-[(S)-2-(Cbz-amino)-3-methylbutanamido]propanamido]-5-fluoro-4-oxopentanoate

(5S,8S,11S)-Methyl 11-(2-fluoroacetyl)-5-isopropyl-8-methyl-3,6,9-trioxo-1-phenyl-2-oxa-4,7,10-triazatridecan-13-oate

Z-VAD-FMK USP/EP/BP

Methyl (5S,8S,11S)-11-(2-fluoroacetyl)-5-isopropyl-8-methyl-3,6,9-trioxo-1-phenyl-2-oxa-4,7,10-triazatridecan-13-oate

Inhibitor,ZVAD(OMe)FMK,inhibit,Caspase,Z VAD(OMe) FMK

Z-Val-Ala-Dl-Asp(Ome)-FMK

Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor

Z-VAD(OMe)-FMK, 10 mM in DMSO

Caspase 3/7 Activity Assay Kit

187389-52-2

67465-36-3

634911-81-9

C22H30FN3O7

C21H28N3O7F

C22H30N3O7F

BioChemical