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Z-VAD-FMK

Product Name
Z-VAD-FMK
CAS No.
187389-52-2
Chemical Name
Z-VAD-FMK
Synonyms
CS-2005;CS-1507;Z-VAD-FMK;HGNC:1504;Z-VAD(OME)-FMK;Z-VA(OME)D-FMK;Z-VADC(OME)-FMK;Z-VAD-FMK, >=98%;Z-VAL-ALA-ASP-FMK;CASPASE INHIBITOR I
CBNumber
CB0203438
Molecular Formula
C22H30FN3O7
Formula Weight
467.49
MOL File
Mol file
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Z-VAD-FMK Property

Melting point:
>190°C (dec.)
Boiling point:
732.4±60.0 °C(Predicted)
Density 
1.214±0.06 g/cm3 (20 ºC 760 Torr)
Flash point:
396.7℃
storage temp. 
-20°C
solubility 
DMSO: 20 mg/mL
form 
powder
pka
11.05±0.46(Predicted)
color 
white
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week.
InChIKey
MIFGOLAMNLSLGH-QOKNQOGYSA-N
SMILES
C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(OC)=O)C(=O)CF
CAS DataBase Reference
187389-52-2
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Safety

Safety Statements 
22-24/25
WGK Germany 
3
HS Code 
29145090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
V116
Product name
Z-VAD-FMK
Purity
solid
Packaging
2mg
Price
$814
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB2501669
Product name
Anti-CASP3 antibody produced in goat
Purity
affinity isolated antibody, buffered aqueous solution
Packaging
100μg
Price
$568
Updated
2024/03/01
Sigma-Aldrich
Product number
627610
Product name
Caspase Inhibitor I
Packaging
1mg
Price
$318
Updated
2024/03/01
Sigma-Aldrich
Product number
627610
Product name
Caspase Inhibitor I
Packaging
5mg
Price
$662
Updated
2024/03/01
Cayman Chemical
Product number
14463
Product name
Z-VAD(OMe)-FMK
Purity
≥95%
Packaging
1mg
Price
$100
Updated
2024/03/01
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Z-VAD-FMK Chemical Properties,Usage,Production

Description

Z-VAD-FMK (187389-52-2), cell-permeable, methyl ester form. Potent, irreversible pan-caspase inhibitor. Inhibits caspase activity and apoptosis induction in a variety of cell types (IC50 = 1.5 μM). Active in vivo.

Uses

Z-VAD(OMe)-FMK is a cell-permeable, competitive, and irreversible inhibitor of all caspases. Through this action, it inhibits cleavage of poly(ADP-ribose) polymerase, preventing apoptosis when used at 10-50 μM. It also blocks caspase-mediated apoptosis in vivo. Z-VAD(OMe)-FMK effectively prevents caspase action in inflammasomes.[Cayman Chemical]

Uses

Z-VAD-FMK is a caspase inhibitor that inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentrations of z-FA-CMK.

Definition

ChEBI: Z-Val-Ala-Asp(OMe)-CH2F is a tripeptide consisting of Z-Val-Ala-Asp(OMe) in which the C-terminal OH group has been replaced by a fluoromethyl group. An irreversible pan-caspase inhibitor. It has a role as an apoptosis inhibitor and a protease inhibitor. It is a carbamate ester, a tripeptide and an organofluorine compound.

General Description

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Biological Activity

Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC 50 = 0.0015-5.8 mM). Active in vivo .

Biochem/physiol Actions

Cell permeable: yes

Enzyme inhibitor

This tripeptide halomethyl ketone (FWfree-acid = 467.49 g/mol; Soluble to 9.35 mg/ml in DMSO; CAS RegistryNumber = 187389-52-2), also known as Z-VAD-FMK and caspase inhibitor VI, is a broad-spectrum caspase inhibitor that blocks caspase-mediated apoptosis. Z-VAD-FMK inhibits caspase processing (IC50 = 0.0015–5.8 mM, depending on enzyme and cell type). The aspartate methyl ester derivative (FW = 467.49 g/mol), also known as caspase inhibitor I, is far more cell-permeable. Target (s) : caspases; caspase-1; caspase-2; caspase-3; caspase-4 ; caspase 5; caspase-6; caspase-7; caspase-8 ; caspase-9; caspase-10.

storage

-20°C

References

1) Slee et al. (1996), Benzyloxycarbonyl-Val-Ala-ASP (OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32; Biochem. J., 315 21 2) Kunstle et al. (1997), ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-alpha; Immunol. Lett., 55 5 3) Garcia-Calvo et al. (1998), Inhibition of human caspases by peptide based and macromolecular inhibitors; J. Biol. Chem., 273 32608

Z-VAD-FMK Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Z-VAD-FMK manufacturers

Henan Aochuang Chemical Co.,Ltd.
Product
Z-VAD-FMK 187389-52-2
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
1ton
Release date
2022-10-08
Career Henan Chemical Co
Product
Z-VAD-FMK 187389-52-2
Price
US $1.00/KG
Min. Order
1G
Purity
98%
Supply Ability
100KG
Release date
2018-08-21

187389-52-2, Z-VAD-FMKRelated Search:


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  • Z-VA(OME)D-FMK
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