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3-METHYLADENINE

Product Name
3-METHYLADENINE
CAS No.
5142-23-4
Chemical Name
3-METHYLADENINE
Synonyms
109723;NSC 66389;NSC-63889;NSC 66389,3-M;3-Methyl-6-am;NSC 66389,3-MA;3-methyl-adenin;3-METHYLADENINE;N3-Methyladenine;Adenine, 3-methyl-
CBNumber
CB9307216
Molecular Formula
C6H7N5
Formula Weight
149.15
MOL File
5142-23-4.mol
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3-METHYLADENINE Property

Melting point:
~300 °C (dec.)(lit.)
Boiling point:
240℃
Density 
1.60
refractive index 
1.8070 (estimate)
Flash point:
99℃
storage temp. 
Keep in dark place,Sealed in dry,Room Temperature
solubility 
Soluble in DMSO (up to 3 mg/ml), or in DMF (up to 10 mg/ml).
pka
6.77±0.20(Predicted)
form 
Powder
color 
White
biological source
synthetic (organic)
BRN 
146087
Stability:
Stable for 2 years from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20° for up to 3 months.
InChI
InChI=1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
InChIKey
FSASIHFSFGAIJM-UHFFFAOYSA-N
SMILES
N1C2C(N(C)C=NC=2N)=NC=1
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Safety

Hazard Codes 
Xn
Risk Statements 
22
Safety Statements 
24/25
WGK Germany 
3
RTECS 
AU6520000
HS Code 
29335990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

P403Store in a well-ventilated place.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
189490
Product name
Autophagy Inhibitor, 3-MA
Packaging
50mg
Price
$155
Updated
2025/07/31
Sigma-Aldrich
Product number
189490
Product name
Autophagy Inhibitor, 3-MA
Packaging
100mg
Price
$171
Updated
2025/07/31
TCI Chemical
Product number
M2518
Product name
3-Methyladenine
Packaging
200mg
Price
$141
Updated
2025/07/31
TCI Chemical
Product number
M2518
Product name
3-Methyladenine
Packaging
1g
Price
$492
Updated
2025/07/31
Cayman Chemical
Product number
13242
Product name
3-Methyladenine
Purity
≥95%
Packaging
25mg
Price
$33
Updated
2024/03/01
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3-METHYLADENINE Chemical Properties,Usage,Production

Description

Autophagy is a multi-step process that involves the degradation and digestion of intracellular components by the lysosome. This process allows cells to efficiently mobilize and recycle cellular constituents, and also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms. mTOR, whose activation is controlled by phosphoinositide 3-kinase (PI3K), is a key regulator of autophagy. 3-Methyladenine (3-MA) is a specific inhibitor of PI3K activity and one of the most widely used inhibitors of the initial phase of the autophagic process: the sequestering of cytoplasmic material by the lysosome. At 5 mM, 3-MA inhibits protein degradation in rat hepatocytes by 65%. 3-MA has been shown to block class I, class II, and class III PI3Ks, including some downstream targets, and to suppress the invasion of highly metastatic human fibrosarcoma HT1080 cells at 10 mM.

Chemical Properties

White powder

Uses

A multi-use inhibitor that protects cerebellar granule cells from apoptosis.

Uses

A synthetic intermediate and a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis post serum/potassium deprivation

Uses

3-Methyladenine (3-MA) inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.

Definition

ChEBI: A methyladenine that is adenine substituted with a methyl group at position N-3.

General Description

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)

Biochem/physiol Actions

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).

storage

+4°C

References

[1] P O SEGLEN  P B G. 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes.[J]. Proceedings of the National Academy of Sciences of the United States of America, 1982, 79 6: 1889-1892. DOI:10.1073/pnas.79.6.1889
[2] EDWARD F. C. BLOMMAART. The Phosphatidylinositol 3-Kinase Inhibitors Wortmannin and LY294002 Inhibit Autophagy in Isolated Rat Hepatocytes[J]. The FEBS journal, 2004, 243 1-2: 240-246. DOI:10.1111/j.1432-1033.1997.0240a.x
[3] AMELIA J. MCFARLAND. Inhibition of autophagy by 3-methyladenine protects 1321N1 astrocytoma cells against pyocyanin- and 1-hydroxyphenazine-induced toxicity[J]. Archives of Toxicology, 2011, 86 2: 275-284. DOI:10.1007/s00204-011-0755-5
[4] X ZHAO. Inhibition of autophagy strengthens celastrol-induced apoptosis in human pancreatic cancer in vitro and in vivo models.[J]. Current molecular medicine, 2014, 14 4: 555-563. DOI:10.2174/1566524014666140414211223

3-METHYLADENINE Preparation Products And Raw materials

Raw materials

Adenine Toluene Nickel Monoperoxyphthalic acid magnesium salt hexahydrate Benzyl bromide N,N-Dimethylacetamide Sulfuric acid Dimethyl sulfate

Preparation Products

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3-METHYLADENINE Suppliers

Americas 1 Belgium 1 Canada 1 CHINA 352 China Hong Kong 1 Europe 1 Germany 1 India 4 Japan 1 Russia 1 Switzerland 2 United Kingdom 5 United States 28 Global 399
Zhongshan Jukemei Biotechnology Co., Ltd.
Tel
13642727863
Email
1009049100@qq.com
Country
China
ProdList
656
Advantage
58
TargetMol Chemicals Inc.
Tel
021-021-33632979 15002134094
Fax
021-33632979
Email
marketing@targetmol.com
Country
China
ProdList
7788
Advantage
58
Nanjing YaoCheng Pharmaceutical Co., Ltd.
Tel
025-57043319 15261831516
Fax
025-57043319
Email
307514552@qq.com
Country
China
ProdList
251
Advantage
50
J & K SCIENTIFIC LTD.
Tel
18210857532; 18210857532
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-021-61259138 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15838
Advantage
69
Chembest Research Laboratories Limited
Tel
+86-21-20908456
Fax
021-58180499
Email
sales@BioChemBest.com
Country
China
ProdList
6003
Advantage
61
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44734
Advantage
61
VDM Biochemicals
Tel
0330-2528181
Fax
0330-2528171
Email
sales@vdmbio.com
Country
United States
ProdList
510
Advantage
64
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View Lastest Price from 3-METHYLADENINE manufacturers

Hebei Chuanghai Biotechnology Co., Ltd
Product
3-METHYLADENINE 5142-23-4
Price
US $10.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
5tons
Release date
2024-12-03
Zibo Hangyu Biotechnology Development Co., Ltd
Product
3-METHYLADENINE 5142-23-4
Price
US $95.00-950.00/kg
Min. Order
10kg
Purity
0.99
Supply Ability
20tons
Release date
2023-11-14
Henan Fengda Chemical Co., Ltd
Product
3-METHYLADENINE 5142-23-4
Price
US $10.00-2.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-28

5142-23-4, 3-METHYLADENINERelated Search:

5-[(4-chlorophenyl)methylidene]-1,3-thiazolidine-2,4-dione NSC 247030 HAMNO NSC23005 Sodium NSC632839hydrochloride NSC228155 S3I-201 5-(4-iodobenzylidene)rhodanine 1,3-Bis(4-aminophenyl)urea 2-Pyridinecarbothioamide, N-2-pyridinyl- Pifithrin-μ 4-Chloro-N,N-diisopropylbenzamide 2,4-Thiazolidinedione,5-[(4-hydroxy-3-methoxyphenyl)methylene]- NSC781406 TIMTEC-BB SBB006342 4-Chloro-N,N-di-n-propylbenzaMide, 97% 4-Chloro-N,N-diethylbenzaMide, 97% 5-(2-HYDROXY-BENZYLIDENE)-THIAZOLIDINE-2,4-DIONE

  • 3H-Purin-6-amine, 3-methyl-
  • 3-methyl-3h-purin-6-amin
  • 3-Methyl-3H-purin-6-amine
  • 3-methyl-adenin
  • Adenine, 3-methyl-
  • 3-METHYLADENINE
  • 3-methyl-3H-adenine
  • 6-AMINO-3-METHYLPURINE
  • Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem
  • 109723
  • 3-methylpurin-6-amine
  • 3-Methyladenine, 90+%, pure
  • 3-Methyladenine, pure
  • 3-Methyl-6-amino-3H-purine
  • 3-Methyladenine6-Amino-3-methylpurine
  • 3-Methyladenine, pure, 90+%
  • 3-Methyl-6-aminopurine
  • N3-Methyladenine
  • NSC 66389
  • 3-MA (3-Methyl adenine)
  • 3-methyladenine,3MA
  • Autophagy Inhibitor, 3-MA
  • NSC 66389,3-MA
  • 3-Methyladenine, GC 90+%
  • NSC 66389,3-M
  • 6-Amino-3-methylpurine 3-Methyladenine
  • Autophagy Inhibitor
  • 3-Methyladenine, >=98%
  • Methyladenine Methyladenine
  • NSC-63889
  • 3-METHYLADENINE USP/EP/BP
  • N3-Methyladenine/NSC 66389
  • 3-Methyl-6-am
  • 3-Methyladenine, selective PI3K inhibitor
  • 3-Methyladenine, 10 mM in DMSO
  • 5142-23-4
  • 12312312-89-6
  • Csub6subHsub7subNsub5sub
  • Building Blocks
  • Biochemicals and Reagents
  • BioChemical
  • Heterocyclic Building Blocks
  • Purines
  • Nucleoside Analogs
  • Nucleosides, Nucleotides, Oligonucleotides
  • Akt
  • mTOR
  • PI3K
  • Amines
  • Bases & Related Reagents
  • Heterocycles
  • Inhibitors
  • Nucleotides