ChemicalBook > CAS DataBase List > Z-VAD-FMK

Z-VAD-FMK

Product Name
Z-VAD-FMK
CAS No.
187389-52-2
Chemical Name
Z-VAD-FMK
Synonyms
CS-2005;CS-1507;Z-VAD-FMK;HGNC:1504;Z-VAD(OME)-FMK;Z-VA(OME)D-FMK;Z-VADC(OME)-FMK;Z-VAD-FMK, >=98%;Z-VAL-ALA-ASP-FMK;CASPASE INHIBITOR I
CBNumber
CB0203438
Molecular Formula
C22H30FN3O7
Formula Weight
467.49
MOL File
Mol file
More
Less

Z-VAD-FMK Property

Melting point:
>190°C (dec.)
Boiling point:
732.4±60.0 °C(Predicted)
Density 
1.214±0.06 g/cm3 (20 ºC 760 Torr)
Flash point:
396.7℃
storage temp. 
-20°C
solubility 
DMSO: 20 mg/mL
form 
powder
pka
11.05±0.46(Predicted)
color 
white
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week.
InChIKey
MIFGOLAMNLSLGH-QOKNQOGYSA-N
SMILES
C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(OC)=O)C(=O)CF
CAS DataBase Reference
187389-52-2
More
Less

Safety

Safety Statements 
22-24/25
WGK Germany 
3
HS Code 
29145090
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
V116
Product name
Z-VAD-FMK
Purity
solid
Packaging
2mg
Price
$814
Updated
2024/03/01
Sigma-Aldrich
Product number
627610
Product name
Caspase Inhibitor I
Packaging
1mg
Price
$318
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB2501669
Product name
Anti-CASP3 antibody produced in goat
Purity
affinity isolated antibody, buffered aqueous solution
Packaging
100μg
Price
$568
Updated
2024/03/01
Sigma-Aldrich
Product number
627610
Product name
Caspase Inhibitor I
Packaging
5mg
Price
$662
Updated
2024/03/01
Cayman Chemical
Product number
14463
Product name
Z-VAD(OMe)-FMK
Purity
≥95%
Packaging
1mg
Price
$100
Updated
2024/03/01
More
Less

Z-VAD-FMK Chemical Properties,Usage,Production

Description

Z-VAD-FMK (187389-52-2), cell-permeable, methyl ester form. Potent, irreversible pan-caspase inhibitor. Inhibits caspase activity and apoptosis induction in a variety of cell types (IC50 = 1.5 μM). Active in vivo.

Uses

Z-VAD(OMe)-FMK is a cell-permeable, competitive, and irreversible inhibitor of all caspases. Through this action, it inhibits cleavage of poly(ADP-ribose) polymerase, preventing apoptosis when used at 10-50 μM. It also blocks caspase-mediated apoptosis in vivo. Z-VAD(OMe)-FMK effectively prevents caspase action in inflammasomes.[Cayman Chemical]

Uses

Z-VAD-FMK is a caspase inhibitor that inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentrations of z-FA-CMK.

Definition

ChEBI: Z-Val-Ala-Asp(OMe)-CH2F is a tripeptide consisting of Z-Val-Ala-Asp(OMe) in which the C-terminal OH group has been replaced by a fluoromethyl group. An irreversible pan-caspase inhibitor. It has a role as an apoptosis inhibitor and a protease inhibitor. It is a carbamate ester, a tripeptide and an organofluorine compound.

General Description

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Biological Activity

Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC 50 = 0.0015-5.8 mM). Active in vivo .

Biochem/physiol Actions

Cell permeable: yes

Enzyme inhibitor

This tripeptide halomethyl ketone (FWfree-acid = 467.49 g/mol; Soluble to 9.35 mg/ml in DMSO; CAS RegistryNumber = 187389-52-2), also known as Z-VAD-FMK and caspase inhibitor VI, is a broad-spectrum caspase inhibitor that blocks caspase-mediated apoptosis. Z-VAD-FMK inhibits caspase processing (IC50 = 0.0015–5.8 mM, depending on enzyme and cell type). The aspartate methyl ester derivative (FW = 467.49 g/mol), also known as caspase inhibitor I, is far more cell-permeable. Target (s) : caspases; caspase-1; caspase-2; caspase-3; caspase-4 ; caspase 5; caspase-6; caspase-7; caspase-8 ; caspase-9; caspase-10.

storage

-20°C

References

1) Slee et al. (1996), Benzyloxycarbonyl-Val-Ala-ASP (OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32; Biochem. J., 315 21 2) Kunstle et al. (1997), ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-alpha; Immunol. Lett., 55 5 3) Garcia-Calvo et al. (1998), Inhibition of human caspases by peptide based and macromolecular inhibitors; J. Biol. Chem., 273 32608

Z-VAD-FMK Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

Z-VAD-FMK Suppliers

China 163 India 2 Japan 1 Switzerland 3 United Kingdom 4 United States 38 Global 211
xinguokeji
Tel
13120711461
Email
363372932@qq.com
Country
China
ProdList
484
Advantage
58
Shanghai Zhengyi Pharmaceutical Co., Ltd
Tel
17740884366
Email
derek1229@163.com
Country
China
ProdList
61
Advantage
58
Jiangsu Shenlang Biotechnology Co., Ltd.
Tel
18914398991
Fax
0513-81001007
Email
1582941412@qq.com
Country
China
ProdList
21
Advantage
58
Hangzhou Peptidego Biotech Co.,Ltd.
Tel
0571-87213919
Fax
0571-87213919
Email
Eric@peptidego.com
Country
China
ProdList
6968
Advantage
58
AdooQ Bioscience CHINA
Tel
025-58849295 18951903616;
Fax
025-68650336
Email
info@adooq.cn
Country
China
ProdList
2990
Advantage
60
Shanghai Boyle Chemical Co., Ltd.
Tel
Fax
86-21-57758967
Email
sales@boylechem.com
Country
China
ProdList
2922
Advantage
55
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15839
Advantage
69
Chembest Research Laboratories Limited
Tel
+86-21-20908456
Fax
021-58180499
Email
sales@BioChemBest.com
Country
China
ProdList
6005
Advantage
61
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
GL Biochem (Shanghai) Ltd
Tel
21-61263452 13641803416
Fax
86-21-61263399
Email
ymbetter@glbiochem.com
Country
China
ProdList
9981
Advantage
64
More
Less

View Lastest Price from Z-VAD-FMK manufacturers

Henan Aochuang Chemical Co.,Ltd.
Product
Z-VAD-FMK 187389-52-2
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
1ton
Release date
2022-10-08
Career Henan Chemical Co
Product
Z-VAD-FMK 187389-52-2
Price
US $1.00/KG
Min. Order
1G
Purity
98%
Supply Ability
100KG
Release date
2018-08-21

187389-52-2, Z-VAD-FMKRelated Search:

Z-FA-FMK Z-DEVD-FMK Z-Vad-fmk, non-methylated 3-METHYLADENINE MK-2206 2HCl Necrostatin-1 MG-132 LY 294002 HYDROCHLORIDE (2S)-2-[(2S)-2-{[(benzyloxy)carbonyl]amino}-3-methylbutanamido]-3-hydroxypropanoic acid PAC1 AC-DEVD-CHO AC-VDVAD-PNA Z-PHE-DL-ALA-FLUOROMETHYLKETONE Z-VAL-ALA-OH Z-VAL-TRP-OH CBZ-L-VALINE METHYL ESTER Z-VAL-VAL-ARG-AMC Z-VAL-VAL-OH

  • Z-VADC(OME)-FMK
  • methyl (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-phenylmethoxycarbonylamino-butanoyl]amino]propanoyl]amino]-4-oxo-pentanoate
  • N-BENZYLOXYCARBONYL-VAL-ALA-ASP FLUOROMETHYL KETONE
  • Z-VAD-FLUOROMETHYLKETONE
  • Z-VAD-FMK
  • Z-VAD-FMK, METHYL ESTER
  • Z-VAD(OME)-FLUOROMETHYLKETONE
  • L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-
  • N-[(Phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-L-alaninamide z-vad -fmk
  • Anti-CASP3 antibody produced in goat
  • caspase 3, apoptosis-related cysteine peptidase
  • caspase 3, apoptosis-related cysteine protease
  • HGNC:1504
  • Z-VAD-FMK, >=98%
  • Z-VAD-FMK, 98%, an irreversible pan-caspase inhibitor
  • Z-VAD(OME)-FMK;Z-VAL-ALA-ASP(OME)-FMK
  • Z-VAD(OME)-FMK
  • Z-VAL-ALA-ASP-FLUOROMETHYLKETONE
  • Z-VAL-ALA-ASP-FMK
  • Z-VAL-ALA-ASP(OCH3)-FLUOROMETHYLKETONE
  • Z-VAL-ALA-ASP(OME)-CH2F
  • Z-VAL-ALA-ASP(OME)-FLUOROMETHYLKETONE
  • Z-VAL-ALA-ASP-(OME)-FMK
  • Z-VAL-ALA-DL-ASP-FLUOROMETHYLKETONE
  • Z-VAL-ALA-DL-ASP(OME)-FLUOROMETHYLKETONE
  • Z-VA(OME)D-FMK
  • N-Benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone
  • BENZYLOXYCARBONYL-VAL-ALA-ASP(OME)-FLUOROMETHYLKETONE
  • CARBOBENZOXY-VALYL-ALANYL-ASPARTYL-[O-METHYL]-FLUOROMETHYLKETONE
  • CASPASE-1, INHIBITOR
  • CASPASE-1 INHIBITOR VI (FLUOROMETHYLKETONE)
  • CASPASE INHIBITOR I
  • CASPASE INHIBITOR VI
  • Z-VAD-FMK, Non-methylated
  • PN: WO2005039629 PAGE: 18 claimed protein
  • Z-VAD-FMK (Cell perMeable)
  • 3: PN: WO0158526 SEQID: 3 claimed sequence
  • N-[(Phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-L-alaninamide
  • Z-VAL-ALA-DL-ASP(OME)-FLUOROMETHYLKETONEZ-Val-Ala-DL-Asp-fluoroMethylketone
  • CS-2005
  • CS-1507
  • Z-VAD-FMK (Z-Val-Ala-Asp(OMe)-FMK)
  • Methyl (S)-3-[(S)-2-[(S)-2-(Cbz-amino)-3-methylbutanamido]propanamido]-5-fluoro-4-oxopentanoate
  • (5S,8S,11S)-Methyl 11-(2-fluoroacetyl)-5-isopropyl-8-methyl-3,6,9-trioxo-1-phenyl-2-oxa-4,7,10-triazatridecan-13-oate
  • Z-VAD-FMK USP/EP/BP
  • Caspase 3/7 Activity Assay Kit
  • Methyl (5S,8S,11S)-11-(2-fluoroacetyl)-5-isopropyl-8-methyl-3,6,9-trioxo-1-phenyl-2-oxa-4,7,10-triazatridecan-13-oate
  • Inhibitor,ZVAD(OMe)FMK,inhibit,Caspase,Z VAD(OMe) FMK
  • Z-Val-Ala-Dl-Asp(Ome)-FMK
  • 187389-52-2
  • 67465-36-3
  • C22H30FN3O7
  • C21H28N3O7F
  • C22H30N3O7F
  • BioChemical
  • Apoptosis and Cell Cycle
  • Cell Biology
  • Cell Signaling and Neuroscience