BIOTIN-NHS

Product Name: BIOTIN-NHS
CAS No.: 35013-72-0
Chemical Name: BIOTIN-NHS
Synonyms: NHS-BIOTIN;BIOTIN-OSU;BNHS;2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]iMidazol-4-yl)pentanoate;(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate;BHSE;BIOTIN SE;BIOTIN-NHS;D-BIOTIN, SE;NHS-D-BIOTIN
CBNumber: CB7371998
Molecular Formula: C14H19N3O5S
Formula Weight: 341.38
MOL File: 35013-72-0.mol

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BIOTIN-NHS Property

Melting point:: 212-214 °C
Density: 1.45±0.1 g/cm3(Predicted)
storage temp.: -20°C
solubility: DMF: ≤50 mg/mL Stable for at least one month in dry DMF
form: powder
pka: 13.89±0.40(Predicted)
color: White to off-white
BRN: 4720781
Stability:: Stable. Incompatible with strong oxidizing agents.
InChI: InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
InChIKey: YMXHPSHLTSZXKH-RVBZMBCESA-N
SMILES: C1(=O)N[C@]2([H])[C@H](CCCCC(ON3C(=O)CCC3=O)=O)SC[C@]2([H])N1
CAS DataBase Reference: 35013-72-0(CAS DataBase Reference)

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Safety

Safety Statements: 22-24/25
WGK Germany: 3
F: 10-23
HS Code: 2934 99 90

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: H1759
Product name: (+)-Biotin N-hydroxysuccinimide ester
Purity: ≥98% (HPLC), powder
Packaging: 5mg
Price: $27.5
Updated: 2025/07/31

Sigma-Aldrich

Product number: H1759
Product name: (+)-Biotin N-hydroxysuccinimide ester
Purity: ≥98% (HPLC), powder
Packaging: 1g
Price: $1030
Updated: 2025/07/31

Sigma-Aldrich

Product number: 8.51023
Product name: Biotin-OSu
Purity: Novabiochem?
Packaging: 1g
Price: $261
Updated: 2025/07/31

Sigma-Aldrich

Product number: 203112
Product name: Biotin-NHS
Packaging: 250MG
Price: $435
Updated: 2025/07/31

TCI Chemical

Product number: S0491
Product name: N-Succinimidyl D-Biotinate
Purity: >97.0%(HPLC)(N)
Packaging: 100mg
Price: $119
Updated: 2025/07/31

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BIOTIN-NHS Chemical Properties,Usage,Production

Description

N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound.

Chemical Properties

White Solid

Uses

amine reactive biotinylation reagent

Uses

Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides.

Uses

Biotin-NHS is used in the biotinylation of proteins and peptides. Couples with primary amine in the pH range 6.5-8.5.

reaction suitability

reaction type: Biotinylations

Synthesis

6066-82-6

58-85-5

35013-72-0

GENERAL STEPS: To a mixed solution of D-biotin (0.82 mmol, 200 mg) and N-hydroxybutanediimide (0.89 mmol, 102 mg) was added 3-(ethyliminomethylamino)-N,N-dimethylpropan-1-amine hydrochloride (0.96 mmol, 184 mg). The reaction mixture was stirred at room temperature overnight and then concentrated to give a white solid. The crude product was mixed with isopropanol, heated to 70 °C and cooled. The pure product was obtained by filtration as a white powder in 91% yield (0.75 mmol, 255 mg).

Purification Methods

Recrystallise the ester from refluxing isoPrOH and dry it in a vacuum over P2O5 + KOH. [Jasiewicz et al. Exp Cell Biol 100 213 1976.]

References

[1] Journal of the American Chemical Society, 2007, vol. 129, # 45, p. 13987 - 13996
[2] New Journal of Chemistry, 2013, vol. 37, # 11, p. 3762 - 3769
[3] Organic Letters, 2013, vol. 15, # 16, p. 4130 - 4133
[4] Journal of Materials Chemistry, 2004, vol. 14, # 17, p. 2638 - 2642
[5] Beilstein Journal of Organic Chemistry, 2015, vol. 11, p. 784 - 791

BIOTIN-NHS Preparation Products And Raw materials

Raw materials

Preparation Products

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BIOTIN-NHS Suppliers

Canada 2 China 368 Europe 2 France 1 Germany 7 India 5 Japan 4 Russia 1 South Korea 1 Switzerland 3 United Kingdom 12 United States 64 Global 470

CHENGDU YINGNUOLING PHARMACEUTICAL TECHNOLOGY CO.,LTD

Tel: 17882285575
Email: 1276966012@qq.com
Country: China
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Heliosense Biotechnologies,Inc

Tel: 0592-5667290 18649641090
Fax: 0592-5667261
Email: info@heliosense.com
Country: China
ProdList: 98
Advantage: 58

Qingdao Vochem Co., Ltd.

Tel: 532-88066095 18661779210
Email: vochem@vochem.com
Country: China
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Shanghai Jizhi Biochemical Technology Co. Ltd.

Tel: 4009004166/18616739031 18616739031
Email: 3007523370@qq.com
Country: China
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Guangzhou Hongyuan Chemical Co.,Ltd

Tel: 15817493340
Email: 981810490@qq.com
Country: China
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Jilin Province Woda Biotechnology Co., Ltd.

Tel: 13504435624
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Country: China
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Anhui Yongsheng Pharmaceutical Technology Co., Ltd

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J & K SCIENTIFIC LTD.

Tel: 18210857532; 18210857532
Fax: 86-10-82849933
Email: jkinfo@jkchemical.com
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Meryer (Shanghai) Chemical Technology Co., Ltd.

Tel: 4006356688 18621169109
Fax: 86-21-61259102
Email: market03@meryer.com
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INTATRADE GmbH

Tel: +49 3493/605464
Fax: +49 3493/605470
Email: sales@intatrade.de
Country: Germany
ProdList: 3572
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View Lastest Price from BIOTIN-NHS manufacturers

HANGZHOU HAILAN CHEMICAL CO.,LTD.

Product: BIOTIN-NHS 35013-72-0
Price: US $0.00/g
Min. Order: 1g
Purity: 99%min
Supply Ability: 2000G
Release date: 2025-10-28

Shaanxi Dideu Medichem Co. Ltd

Product: BIOTIN-NHS 35013-72-0
Price: US $1.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20T
Release date: 2024-08-09

Xian confluore Biological Technology Co., Ltd.

Product: Biotin-NHS ester 35013-72-0
Price: US $0.00/g
Min. Order: 25g
Purity: >95.00%
Supply Ability: 25g
Release date: 2024-03-30

35013-72-0, BIOTIN-NHSRelated Search:

Sulfo-NHS-Biotin BIOTIN-XX-NHS Succinimidyl 6-(biotinamido)hexanoate N-Hydroxysuccinimide BIOTIN DISULFIDE N-HYDROXY-SUCCINIMIDE ESTER methyl 5-[(1S,2S,5R)-7-oxo-3-thia-6,8-diazabicyclo[3.3.0]oct-2-yl]pent anoate BIOTIN HYDRAZIDE (+)-BIOTIN-EPSILON-AMINOCAPROIC ACID D-BIOTIN P-NITROPHENYL ESTER Biotin-NHCH2CH2-SS-Pyr Biotin-SLC-NHS Succinimide BIOTIN-NHS DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER 2,5-Dioxopyrrolidin-1-yl octanoate D-DESTHIOBIOTIN D-biotin-d-sulfoxide 7,8-DIAMINOPELARGONIC ACID

(+)-biotin-n-hydroxysuccimide ester

n-hydroxysuccimidobiotin

BIOTIN-OSU: N-HYDROXYSUCCINIMIDOBIOTIN

d-Biotin NHS ester

(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate

5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER

D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER

D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER

D-BIOTIN, SE

D-BIOTIN, SUCCINIMIDYL ESTER

D-BIOTINYL-OSU

BNHS

BIOTIN-OSU

BIOTIN-N-HYDROXYSUCCINIMIDE

(+)-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER

BIOTIN-N-HYDROXYSUCCINIMIDE ESTER

BIOTIN-NHS

BIOTIN (LONG ARM) NHS

BIOTIN SE

BIOTIN SUCCINIMIDYL ESTER

BIOTINYL-N-HYDROXY-SUCCINIMIDE

(+)-BIOTINYL-N-SUCCINIMIDYL ESTER

SUCCINIMIDO-(+)-BIOTIN

SUCCINIMIDYL D-BIOTIN

N-HYDROXYSUCCINIMIDOBIOTIN

NHS-BIOTIN

NHS-D-BIOTIN

N-SUCCINIMIDYL D-BIOTINATE

N-SUCCINIMIDO-(+)-BIOTIN

N-Hydroxysuccinimidyl)biotin

(+)-Biotin-NHS Ester

2,5-Dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)

2,5-Dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentano

Biotin-OSu Novabiochem

Biotin, succinimidyl ester *CAS 35013-72-0*

(+)-Biotin-NHS:BIOTIN-NHS

N-Hydroxysuccinimido Biotin、BNHS

N-Succinimidyl-D-biotin

D-Biotin N-Succinimidyl Ester

N-(D-Biotinyloxy)succinimide

(+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, Biotinyl-N-hydroxy-succinimide, BNHS, N-Hydroxysuccinimidobiotin, N-Succinimidyl D-biotinate, NHS-Biotin

1-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-2,5-pyrrolidinedione

BHSE

(+)-Biotin N-hydroxysuccinimide ester

Biotin-NHS N-Hydroxysuccimidobiotin

(+)-Biotin N-hydroxysuccinimide ester,(+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, BNHS, Biotinyl-N-hydroxy-succinimide, N-Hydroxysuccinimidobiotin, N-Succinimidyl D-biotinate, NHS-Biotin

BIOTIN-NHS (+)-Biotin N-hydroxysucciniMide ester

2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]iMidazol-4-yl)pentanoate

Hexahydro-2-oxo-

D-Biotin N-Succinimidyl Ester N-(D-Biotinyloxy)succinimide

BNHS, Biotin-NHS

BNHS/NHS-Biotin

(+)-Biotin N-hydroxysuccinimide ester(Biotin-NHS)

2,5-Dioxo-1-pyrrolidinyl 5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoate

Biotin NHS【2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate】

CAS_35013-72-0

Biotin-N-succinimidyl

N-SuccinimidylD-Biotinate&gt