Y16
- Product Name
- Y16
- CAS No.
- 429653-73-6
- Chemical Name
- Y16
- Synonyms
- Y16;Y16, >98%;Y16, 429653-73-6;Y16, 10 mM in DMSO;Rho Inhibitor II, Y16;Y16 (Rho GEFs inhibitor Y16);Inhibitor,inhibit,Y-16,Y 16,Ras,Y16;4-(3-((3-Methylbenzyl)oxy)benzylidene)-1-phenylpyrazolidine-3,5-dione;(e)-4-(3-((3-methylbenzyl)oxy)benzylidene)-1-phenylpyrazolidine-3,5-dione;4-[[3-[(3-Methylphenyl)methoxy]phenyl]methylene]-1-phenyl-3,5-pyrazolidinedione
- CBNumber
- CB92735545
- Molecular Formula
- C24H20N2O3
- Formula Weight
- 384.43
- MOL File
- 429653-73-6.mol
Y16 Property
- Density
- 1.273±0.06 g/cm3(Predicted)
- storage temp.
- 2-8°C
- solubility
- DMSO: soluble5mg/mL, clear (warmed)
- pka
- 8.84±0.20(Predicted)
- form
- powder
- color
- , faint yellow to dark orange
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H410Very toxic to aquatic life with long lasting effects
- Precautionary statements
-
P273Avoid release to the environment.
P391Collect spillage. Hazardous to the aquatic environment
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- SML0873
- Product name
- Y16
- Purity
- ≥98% (HPLC)
- Packaging
- 25mg
- Price
- $110
- Updated
- 2025/07/31
- Product number
- SML0873
- Product name
- Y16
- Purity
- ≥98% (HPLC)
- Packaging
- 5mg
- Price
- $121
- Updated
- 2025/07/31
- Product number
- CS-3879
- Product name
- Y16
- Purity
- 99.03%
- Packaging
- 10mg
- Price
- $144
- Updated
- 2021/12/16
- Product number
- CS-3879
- Product name
- Y16
- Purity
- 99.03%
- Packaging
- 5mg
- Price
- $84
- Updated
- 2021/12/16
- Product number
- CD31001921
- Product name
- Y16
- Purity
- 98+%
- Packaging
- 5mg
- Price
- $49
- Updated
- 2021/12/16
Y16 Chemical Properties,Usage,Production
Uses
Y16 is a specific inhibitor of Leukemia-associated Rho guanine nucleotide exchange factor (LARG) with a Kd value of 76 nM. Y16 is active in blocking the interaction of LARG and related G-protein-coupled Rho GEFs with RhoA. Y16 shows no detectable effect on other diffuse B-cell lymphoma (Dbl) family Rho GEFs, Rho effectors, or a RhoGAP[1].
Biological Activity
Y16 is an inhibitor of G protein-coupled Rho GEFs. It blocks the binding of LARG and Rho with a Kd of 80 nM.
in vitro
Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) could inhibit RhoA-GTP formation induced by serum dose dependently and is specific for RhoA.
Y16 (10-30 μΜ; 24 hours ; NIH 3T3 cells) efficiently inhibits serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which are downstream of RhoA.
Cell Viability Assay
| Cell Line: | NIH 3T3 cells |
| Concentration: | 10 μΜ, 30 μΜ |
| Incubation Time: | 24 hours |
| Result: | Inhibited RhoA-GTP formation induced by serum dose dependently and was specific for RhoA. |
Western Blot Analysis
| Cell Line: | NIH 3T3 cells |
| Concentration: | 10 μΜ, 30 μΜ |
| Incubation Time: | 24 hours |
| Result: | Inhibited serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which were downstream of RhoA. |
target
| Target | Value |
| RhoGEFs () |