ChemicalBook > CAS DataBase List > Bromothymol Blue

Bromothymol Blue

Product Name
Bromothymol Blue
CAS No.
76-59-5
Chemical Name
Bromothymol Blue
Synonyms
BTB;BL;BROMOTHYMOL BLUE SOLUTION;Nsc 7819;BETZ 0417;NSC 42609;ol)s,s-dioxide;BROMTHYMOL BLUE;Bromothmol blue;yl)-,s,s-dioxide
CBNumber
CB9671368
Molecular Formula
C27H28Br2O5S
Formula Weight
624.38
MOL File
76-59-5.mol
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Bromothymol Blue Property

Melting point:
200-202 °C (lit.)
Density 
1.4668 (estimate)
bulk density
450kg/m3
Flash point:
38 °C
storage temp. 
Store at +5°C to +30°C.
solubility 
Solubility Sparingly soluble in water, benzene; soluble in ethanol, ether; insoluble in petroleum ether
form 
Solid
pka
7.0, 7.1(at 25℃)
Boiling point:
614.3±55.0 °C(Predicted)
color 
Pink-brown to purple
PH
pH : 6.0～7.6
PH Range
6(yellow)-7.6(blue)
Odor
Characteristic odour
Water Solubility 
slightly soluble
λmax
420nm, 435nm, 620nm
Merck 
14,1445
BRN 
373934
Stability:
Stable. Incompatible with strong oxidizing agents.
Major Application
Sol-gel matrix, fuel cells, optical sensors, combustion gas detection system, paints, toys, cleaning products, detergents, textiles, food freshness sensor, identifying fresh and stale rice,wheat, detecting microorganisms, bacterial growth, psychoactive drug, dental materials
LogP
8.600 (est)
CAS DataBase Reference
76-59-5(CAS DataBase Reference)
EPA Substance Registry System
Bromothymol blue (76-59-5)
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Safety

Hazard Codes 
Xn
Risk Statements 
10-41-36/37/38-20/21/22
Safety Statements 
24/25-39-26-36
RIDADR 
1170
WGK Germany 
3
RTECS 
SJ450000
TSCA 
Yes
HazardClass 
3
PackingGroup 
III
HS Code 
29349990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H226Flammable liquid and vapour

H319Causes serious eye irritation

H340May cause genetic defects

H360May damage fertility or the unborn child

H372Causes damage to organs through prolonged or repeated exposure

H373May cause damage to organs through prolonged or repeated exposure

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P233Keep container tightly closed.

P240Ground/bond container and receiving equipment.

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P308+P313IF exposed or concerned: Get medical advice/attention.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
114413
Product name
Bromothymol Blue
Purity
ACS reagent, Dye content 95%
Packaging
5g
Price
$36.8
Updated
2024/03/01
Sigma-Aldrich
Product number
114413
Product name
Bromothymol Blue
Purity
ACS reagent, Dye content 95%
Packaging
25g
Price
$127
Updated
2024/03/01
Sigma-Aldrich
Product number
1.03026
Product name
Bromothymol blue
Purity
indicator ACS,Reag. Ph Eur
Packaging
5G
Price
$143
Updated
2024/03/01
Sigma-Aldrich
Product number
1.03026
Product name
Bromothymol blue
Purity
indicator ACS,Reag. Ph Eur
Packaging
25g
Price
$357
Updated
2024/03/01
Sigma-Aldrich
Product number
BX1555
Product name
Bromothymol Blue
Purity
meets analytical specifications of USP/NF GR ACS
Packaging
25g
Price
$236
Updated
2022/05/15
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Bromothymol Blue Chemical Properties,Usage,Production

Description

Bromothymol blue is a pH indicator for weak acids and bases. In neutral solution, it appears bluish-green. At pH above 7.6 it appears blue, and at pH below 6.0 it appears yellow.
Bromothymol blue (BTB) is an anionic dye. It can be protonated or deprotonated to generate yellow or blue. BTB helps to make ion-pair complex, that is used to identify several pharmaceutical compounds with the help of extractive spectrophotometric methods.

Chemical Properties

Pale purple crystalline powder

Uses

Bromothymol blue is a chemical indicator for weak acids and bases.

Uses

Bromothymol blue is mostly used in measuring substances that would have relatively low acidic or basic levels (near a neutral pH). It's often used in pools, fish tanks, or measuring the presence of carbonic acid in a liquid.
Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Uses

Bromothymol Blue is a pH indicator used for measuring pH around 7 in pools and fish tanks. It operates in the pH range 6.0 to 7.6 due to the presence of an electron withdrawing and donating groups like bromine atoms and alkyl substitutents respectively. In the laboratory, it is used as a biological slide stain as well as used in obstetrics for detecting premature rupture of membranes. Further, it is used for the observation of photosynthetic activities and a respiratory indicator. In addition to this, it is used to define cell walls or nuclei under the microscope.

Application

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.
Suitable for use:
In preparations of pH indicator solutions.
In phospholipid staining on tissues and cells prepared on microscopy slides.

Preparation

Bromothymol blue is synthesized by adding elemental bromine to the thymol blue in a solution of glacial acetic acid.
To prepare a solution that is used as a pH indicator, we should dissolve 0.10 g in an 8.0 cm3 N/50 NaOH and then dilute it with water to 250 cm3. To prepare a solution used as an indicator in volumetric work, we should dissolve 0.1 g in the 100 cm3 of 50% (v/v) ethanol.

Definition

ChEBI: A member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-isopropyl-2-methylphenyl groups.

General Description

Bromothymol blue is a pH indicator which is sparingly soluble in water, benzene but is soluble in ethanol. It is insoluble in petroleum ether. It has its major uses in Sol-gel matrix, fuel cells, optical sensors, combustion gas detection system. It is considered as a toxic reagent.

Purification Methods

Dissolved the dye in aqueous 5% NaHCO3 solution and precipitate it from the hot solution by dropwise addition of aqueous HCl. Repeat this until the extinction at max 420nm does not increase. It is an indicator: aqueous solutions are yellow at pH 6.0, and blue at pH 7.6. [Beilstein 19/3 V 461.]

Bromothymol Blue Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Bromothymol Blue manufacturers

HebeiShuoshengImportandExportco.,Ltd
Product
Bromothymol Blue 76-59-5
Price
US $6.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
2000KG/Month
Release date
2024-08-07
Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Bromothymol Blue 76-59-5
Price
US $85.00-25.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20ton
Release date
2024-05-22
Hebei Longbang Technology Co., Ltd
Product
Bromothymol Blue 76-59-5
Price
US $6.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
20TONS
Release date
2024-05-13

76-59-5, Bromothymol BlueRelated Search:

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