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demethylzeylasteral

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demethylzeylasteral Basic information

Product Name:
demethylzeylasteral
Synonyms:
  • demethylzeylasteral
  • (9beta,13alpha,14beta,20alpha)-2,3-Dihydroxy-9,13-dimethyl-6,23-dioxo-24,25,26-trinoroleana-1,3,5(10),7-tetraen-29-oic acid
  • A-Friedo-24-noroleana-1,3,5(10),7-tetraen-29-oicacid
  • Demethylzeylasteral(T-96)
  • 24,25,26-Trinoroleana-1,3,5(10),7-tetraen-29-oic acid, 2,3-dihydroxy-9,13-dimethyl-6,23-dioxo-, (9β,13α,14β,20α)-
  • (2R,4AS,6aS,12bR,14aS,14bR)-9-formyl-10,11-dihydroxy-2,4a,6a,12b,14a-pentamethyl-8-oxo-1,2,3,4,4a,5,6,6a,8,12b,13,14,14a,14b-tetradecahydropicene-2-carboxylic acid
  • Demethylzeylasteral-RM
  • Demethylzeylasteral, 10 mM in DMSO
CAS:
107316-88-1
MF:
C29H36O6
MW:
480.59
Product Categories:
  • chemical reagent
  • pharmaceutical intermediate
  • phytochemical
  • reference standards from Chinese medicinal herbs (TCM).
  • standardized herbal extract
Mol File:
107316-88-1.mol
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demethylzeylasteral Chemical Properties

Melting point:
>221°C (dec.)
Boiling point:
663.6±55.0 °C(Predicted)
Density 
1.31
storage temp. 
under inert gas (nitrogen or Argon) at 2-8°C
solubility 
DMSO (Slightly), Methanol (Slightly)
form 
Solid
pka
4.78±0.70(Predicted)
color 
Yellow
Major Application
food and beverages
InChIKey
ZDZSFWLPCFRASW-CPISFEQASA-N
SMILES
Oc1c(c2c(cc1O)[C@@]3(CC[C@]4([C@H]5[C@@](CC[C@@]4(C3=CC2=O)C)(CC[C@@](C5)(C)C(=O)O)C)C)C)C=O
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Safety Information

WGK Germany 
WGK 3
Storage Class
11 - Combustible Solids
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demethylzeylasteral Usage And Synthesis

Chemical Properties

White crystalline powder, soluble in organic solvents such as methanol, ethanol, and DMSO, derived from Tripterygium wilfordii.

Uses

Demethylzeylasteral is a natural product as new inhibitor to block the Bcl-XL-Bid interaction. Also, it is an immunosuppressive monomer isolated from the root xylem of Tripterygium wilfordii. It exhibits inhibition towards the glucuronidation elimination reaction of SN-38.

Definition

ChEBI: A carbopolycyclic compound with formula C29H36O6, originally isolated from Tripterygium wilfordii.

Synthesis

Lei Gong Teng herbs crushed, weighed 500g, add 3L 70% ethanol ultrasonic extraction twice, extraction time for 20min, power 500W, combined extracts, concentrated to extract, add 200mlpH8 of sodium bicarbonate solution to dissolve, filtration, filtrate with . Hydrochloric acid adjusted to pH 6.5, add 300 ml of ethyl acetate extraction 2 times, combined with the extract, evaporated to get the crude demethyl zeylanol aldehyde; demethyl zeylanol aldehyde crude dissolved in methanol, microporous filtration membrane filtration, by the preparation of high-performance liquid chromatographic separation, chromatographic column: octadecyl bond and silica gel, mobile phase: acetonitrile - water (40:60), to monitor the guidance of the refractive detector monitoring The product was collected, and the collected liquid was concentrated and dried to obtain 1.42g, the content was 98.7%.

in vivo

Demethylzeylasteral (30 mg/kg, 6 times every 2 days, i.p.) inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis[1]. Demethylzeylasteral (4 mg/kg, 5 weeks, i.p.) inhibits the invasion of triple negative breast cancer by blocking classical and non classical TGF - β signaling pathways[2]. Demethylzeylasteral (30-120 mg/kg, 8 weeks, i.p.) improves inflammation in a unilateral ureteral obstruction rat model by inhibiting the activation of the NF - κ B pathway[4]. Demethylzeylasteral (10, 40 mg/kg, 30 days, i.g.) can alleviate atherosclerosis in AS rabbits[5]. Demethylzeylasteral (10, 20 mg/kg, 4 weeks, p.o.) improves CCl4 induced liver fibrosis in mice by inhibiting AGAP2 mediated FAK/AKT signaling[6].

Animal Model:Female nude mice modeled with glioma LN-229 cells[1].
Dosage:30 mg/kg, 6 times every 2 days
Administration:Intraperitoneal injection (i.p.)
Result:Reduced tumor volume
Animal Model:Female BALB/c mice modeled with 4T1 cells[2].
Dosage:4 mg/kg, 5 weeks
Administration:Intraperitoneal injection (i.p.)
Result:Reduced cancer lung metastasis
Animal Model:Rat model of unilateral ureteral obstruction[4].
Dosage:30-120 mg/kg, 8 weeks
Administration:Intraperitoneal injection (i.p.)
Result:Inhibited the increases in serum creatinine, blood urea nitrogen and Up/Ucr ratio
Animal Model:Atherosclerotic rabbit[5].
Dosage:10, 40 mg/kg, 30 days
Administration:Intragastrical (i.g.)
Result:Reduced blood lipids triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL-C)
Animal Model:CCl4-induced liver fibrosis model[6].
Dosage:10, 20 mg/kg, 4 weeks
Administration:Oral gavage (p.o.)
Result:Inhibited the expression of TGF-β1

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