TCO-PEG4-NHS Ester Chemical Properties

Density 

1.23±0.1 g/cm3(Predicted)

storage temp. 

Storage temp. -20°C

solubility 

DMSO, DMF, THF, DCM, Acetonitrile

pka

11.95±0.46(Predicted)

form 

Oil

color 

Colorless to light yellow

Appearance

colorless syrup

TCO-PEG4-NHS Ester Usage And Synthesis

Description

TCO-PEG4-NHS ester is a bifunctional linker with trans-cyclooctene (TCO) and NHS ester groups flanking PEG4 (tetraethylene glycol).

The PEG spacer increases solubility in aqueous media and provides a long and flexible connection that minimizes steric hindrance involved with ligation.

The amine-reactive N-hydroxysuccinimide residue provides easy attachment to almost any primary or secondary amine group, such as protein, peptide, or small molecule amine.

Trans-cyclooctene readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term aqueous stability, which is important in low-concentration applications such as protein-protein conjugations, etc.

Uses

TCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1]. TCO-PEG4-NHS ester is a click chemistry reagent, it contains a TCO group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing Tetrazine groups.

IC 50

PEGs

References

[1] Nalawansha DA, et al. PROTACs: An Emerging Therapeutic Modality in Precision Medicine. Cell Chem Biol. 2020;27(8):998-1013. DOI:10.1016/j.chembiol.2020.07.021

TCO-PEG4-NHS Ester(1621096-79-4)Related Product Information

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• TCO-PEG4-Acid
• TCO4-PEG7-NH2
• TCO4-PEG3-NHS
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• TCO4-PEG7-Maleimide
• TCO4-PEG2-NH2