AURAMINE O Chemical Properties

Melting point:

>250 °C (dec.)(lit.)

storage temp. 

Inert atmosphere,Room Temperature

solubility 

10g/l

form 

Powder/Solid

pka

9.8, 10.7(at 25℃)

Colour Index 

41000

color 

Yellow to ochre-yellow

PH

6-7 (10g/l, H2O, 20℃)

PH Range

6.5

Water Solubility 

Soluble in water or ethanol

λmax

370 nm, 432 nm

BRN 

4030061

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Detecting bacteria; treatment of cancers,cardiovascular disease,diabetesassociated pain,mechanical allodynia,metabolic syndrome,protozoan infections in fish; targeted drug delivery; as fungal inhibitors

CAS DataBase Reference

2465-27-2(CAS DataBase Reference)

NIST Chemistry Reference

Aniline, 4,4'-(imidocarbonyl)bis-(n,n-dimethyl)-, hydrochloride(2465-27-2)

EPA Substance Registry System

Auramine, monohydrochloride (2465-27-2)

Safety Information

Hazard Codes 

T,Xn

Risk Statements 

22-24-40-20/21/22

Safety Statements 

36/37-45-36-22

RIDADR 

UN 2811 6.1/PG 3

WGK Germany 

3

RTECS 

BY3675000

TSCA 

Yes

HS Code 

3204 13 00

HazardClass 

6.1

PackingGroup 

III

Hazardous Substances Data

2465-27-2(Hazardous Substances Data)

Toxicity

LD50 orally in Rabbit: 1000 mg/kg

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich

AURAMINE O Usage And Synthesis

Chemical Properties

yellow powder

Chemical Properties

Auramine is a yellow crystalline powder or flaky material.

Uses

Certified for use by fluorescence microscopy in Churukian's modification of Truant's fluorescent method for acid fast bacilli on paraffin sections.

Uses

Fluorescent stain for acid-resistant bacteria in sputum

Definition

ChEBI: A hydrochloride obtained by combining 4,4'-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. A fluorescent stain for demonstrating acid fast organisms in a method similar to the Zieh Neelsen. It also can be used to make a fluorescent Schiff reagent.

General Description

Yellow flakes or powder.

Air & Water Reactions

Insoluble in water.

Reactivity Profile

AURAMINE O is incompatible with strong oxidizing agents.

Hazard

Probable carcinogen.

Fire Hazard

Flash point data for AURAMINE O are not available. AURAMINE O is probably combustible.

Potential Exposure

Auramine is used industrially as a dye or dye intermediate for coloring textiles; paper, and leather. Also used as an antiseptic (a powerful antiseptic in ear and nose surgery, and treatment of gonorrhea) and fungicide. Human exposure to auramine occurs principally through skin absorption or inhalation of vapors. Low-level dermal exposure to the consumer may occur but would be limited to any migration of auramine from fabric, leather, or paper goods.

Shipping

UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required. UN3143 Dyes, solid, toxic, n.o.s. or Dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.

Properties and Applications

TEST ITEMS	SPECIFICATION
APPEARANCE	DARK YELLOWPOWDER
SHADE	BRIGHT YELLOWISH
HEAT RESISTANCE	280 °C min
DENSITY	1.07 g/cm 3
WATER FASTNESS	4-5
LIGHT FASTNESS	1-2
BLEACHABILITY (OXIDATIVE)	4
BLEACHABILITY (REDUCTIVE)	3
WATER SOLUBILITY AT 20 °C	10 g/L min
WATER INSOLUBLE	1.0% max
MOISTURE	3.0% max
TINTING STRENGTH	100-105 %
WEIGHT METAL TOTAL	50ppm max

Purification Methods

It crystallises from EtOH as the hydrochloride and is very slightly soluble in CHCl3, UV: 434nm ( 370). The free base (carbinolamine) has m 136o (from *C6H6). [Goldacre & Phillips J Chem Soc 1724 1949, Conrad et al. Biochemistry 9 1540 1970, Beilstein 14 IV 256.]

Incompatibilities

Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.

Waste Disposal

Consult with environmental regulatory agencies for guidance on acceptable disposal practices. Generators of waste containing this contaminant (≥100 kg/mo) must conform with EPA regulations governing storage, transportation, treatment, and waste disposal. Incinerate in furnace with afterburner and scrubber.

AURAMINE O(2465-27-2)Related Product Information

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• 4,4'-Methylenebis(N,N-dimethylaniline)
• N-METHYL-4,4'-METHYLENEDIANILINE
• AURAMINE O
• AURAMINE（LIQUID）
• Auramine Base,AURAMINE(TECHNICAL-GRADE)
• Michler's ketone
• 4,4'-METHYLENEBIS(N-METHYLANILINE)
• AURAMINE G
• Auramine,monohydrate
• Auramine Fluorochrome Kit, Mycobacteria Fluorescent Stain Kit
• Auramine/Phenol 10x conc.
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• AURAMINE BASE (C.I. 41000:1)
• CALCOZINE AURAMINE OO
• DUPONT AURAMINE SP
• Auramine Phenol
• Auramine Decolouriser (Lempert)