5-Bromo-3-indolyl-beta-D-galactopyranoside Chemical Properties

Melting point:

195 °C

Boiling point:

646.6±55.0 °C(Predicted)

alpha 

-34.5 º (c=1% in DMF/H2O 1:1)

Density 

1.824±0.06 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

DMF: soluble

form 

Powder

pka

12.82±0.70(Predicted)

color 

White

optical activity

[α]20/D 34.5±2.0°, c = 1% in DMF/H2O 1:1

Water Solubility 

Soluble in dimethylformamide (100 mg/ml), dimethylformamide/water (1:1 v/v) (10 mg/ml - clear, practically colorless solution).

BRN 

1550140

CAS DataBase Reference

97753-82-7(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22-36/37/38

Safety Statements 

22-24/25-36-26

WGK Germany 

3

HS Code 

29389090

MSDS

• Language:English Provider:SigmaAldrich

5-Bromo-3-indolyl-beta-D-galactopyranoside Usage And Synthesis

Chemical Properties

White to off-white powder

Uses

5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.

Uses

5-Bromo-3-indolyl-beta-D-galactopyranoside is a substrate for beta-galactosidase that is converted, by the enzyme, into an insoluble indigoblue chromophore. It is also used in Lac gene detection systems, in immunoblotting and immunocytochemical assays.

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