5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Chemical Properties

Melting point:

230 °C

alpha 

-64 º (C=1, H2O/DMF (1/1))

Boiling point:

673.9±55.0 °C(Predicted)

Density 

1.5563 (rough estimate)

refractive index 

1.6110 (estimate)

storage temp. 

-20°C

solubility 

Soluble in 100 mM in DMSO.

form 

Powder

pka

12.74±0.70(Predicted)

color 

White

Odor

Odorless

Sensitive 

Moisture & Light Sensitive

Merck 

14,10074

BRN 

1402009

Stability:

Stable. Incompatible with strong oxidizing agents.

InChIKey

OPIFSICVWOWJMJ-AEOCFKNESA-N

CAS DataBase Reference

7240-90-6(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

24/25-37/39-26

WGK Germany 

3

F 

8-10-21

HazardClass 

IRRITANT

HS Code 

29389090

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Usage And Synthesis

Chemical Properties

White Powder

Uses

A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm).

Uses

magenta b-galactosidase substrate

Uses

A substrate for -Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm)

Uses

In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.

Definition

ChEBI: An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for he presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product.

storage

+4°C

5-Bromo-4-chloro-3-indolyl-beta-D-galactoside(7240-90-6)Related Product Information

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