tert-Butoxycarbonyl-L-valine N-hydroxysuccinimide ester Chemical Properties

Melting point:

126-128 °C

Density 

1.22±0.1 g/cm3(Predicted)

storage temp. 

-20°C

pka

10.72±0.46(Predicted)

form 

Powder

color 

White to off-white

optical activity

[α]20/D 38±1°, c = 2% in dioxane

Sensitive 

Moisture Sensitive

BRN 

1551859

CAS DataBase Reference

3392-12-9(CAS DataBase Reference)

Safety Information

WGK Germany 

3

F 

3-10-21

HS Code 

2933998090

MSDS

• Language:English Provider:SigmaAldrich

tert-Butoxycarbonyl-L-valine N-hydroxysuccinimide ester Usage And Synthesis

Chemical Properties

White powder

Uses

peptide synthesis

reaction suitability

reaction type: Boc solid-phase peptide synthesis

Synthesis

Step A: Boc-L-valine (1.03 g, 4.74 mmol), N-hydroxysuccinimide (1.22 g, 10.6 mmol), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, 1.60 g, 8.35 mmol) were sequentially added to a dry round-bottom flask. The above reagents were dissolved in anhydrous dichloromethane (30 mL), and the reaction system was sealed through a rubber septum and displaced three times with argon to exclude oxygen. Subsequently, the reaction mixture was stirred at room temperature.After 3 days, thin layer chromatography (TLC) detection (ninhydrin color development) confirmed that the Boc-L-valine was fully reacted. The reaction solution was washed sequentially with water, saturated aqueous sodium bicarbonate, and the aqueous phase was back-extracted with dichloromethane. The organic phases were combined, washed with saturated saline, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure and dried under vacuum to obtain white solid Boc-L-valine hydroxysuccinimide ester (1.52 g, 100% yield). The product was characterized by 1H-NMR (300 MHz, CDCl3): δ 4.98 (broad peak, 1H), 4.58 (double peak, 1H), 2.82 (multiple peaks, 4H), 2.27 (multiple peaks, 1H), 1.44 (single peak, 9H), 1.03 (double peaks, 6H).

References

[1] Patent: WO2016/160615, 2016, A1. Location in patent: Paragraph 0444; 0445
[2] Journal of Medicinal Chemistry, 1998, vol. 41, # 14, p. 2461 - 2480
[3] Journal of Medicinal Chemistry, 1991, vol. 34, # 9, p. 2852 - 2857
[4] Journal of the Chemical Society, Dalton Transactions: Inorganic Chemistry (1972-1999), 1984, p. 2305 - 2308
[5] Journal of Organic Chemistry, 1986, vol. 51, # 24, p. 4580 - 4585

tert-Butoxycarbonyl-L-valine N-hydroxysuccinimide ester(3392-12-9)Related Product Information

• PYRAZOPHOS
• 1,1'-Carbonyldiimidazole
• O-Phthalimide
• Dibutyl phthalate
• Dimethyl succinate
• L-Valine
• Methyl acrylate
• Cyhalofop-butyl
• Butyl acetate
• Maleimide
• Bismaleimide
• Valine
• CARBONYL SULFIDE
• tert-Butanol
• Tris(trimethylsilyl)phosphate
• BUTYL OLEATE
• Succinimide
• Diethylene glycol monobutyl ether