5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN Chemical Properties

Melting point:

152-154 °C(lit.)

storage temp. 

-20°C

solubility 

DMF: soluble

pka

6.45(at 25℃)

form 

Solid

color 

Off-white to yellow

Appearance

white solid

PH Range

Weak green ' uorescence (6.0) to strong green ' uorescence (7.2)

λmax

<300nm

Major Application

Diagnosis of cancer, restricting HIV replication, antiinflammatory agent, detecting bacteria, cell proliferation assays

InChIKey

QSWYNOQSGMUDQZ-UHFFFAOYSA-N

SMILES

C12(OC(=O)C3=CC(C(=O)ON4C(=O)CCC4=O)=CC=C13)C1C=CC(OC(=O)C)=CC=1OC1C=C(OC(=O)C)C=CC2=1

Safety Information

WGK Germany 

3

F 

8-10-21

HS Code 

32129000

MSDS

• Language:English Provider:SigmaAldrich

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN Usage And Synthesis

Description

(5,6)-FAM diacetate NHS ester is a cell-permeable amine-reactive label for biomolecules.

This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore with emission in the green spectrum range (absorption maximum at ~492 nm, emission maximum at ~517 nm). Due to this characteristic, this product is used for tracking cells and flow cytometric evaluations and studying intracellular esterase.

Chemical Properties

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is Off-white to light yellow powder

Uses

Amine-reactive succinimidyl ester can potentially be used for long-term pH studies of live cells, producing a conjugate with the pH-sensitive properties of carboxyfluorescein.

Uses

A 5(6)-Carboxyfluorescein (C178215) derivative used as a flurorescent probe. 5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester is used to monitor lymphocyte proliferation by covalently labelling long-lived intracellular molecules with fluorescent dye.

Uses

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is a Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell.

General Description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

Abs/Em Maxima

492/517 nm

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN(150347-59-4)Related Product Information

• 6-Carboxyfluorescein
• 5-CFDA N-succinimidyl ester
• 6-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester
• BIS-(4-METHOXYPHENYL) ETHER
• 4-(2 5-DIMETHOXYPHENYL)BUTYRIC ACID 97
• TERT-BUTYL 3-BUTENOATE
• 3,5-BIS(CHLOROMETHYL)PYRIDINE HCL
• 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
• GAMMA-PHENYL-GAMMA-BUTYROLACTONE
• 4,4'-Oxydiphenol
• 6-CARBOXYFLUORESCEIN DIACETATE
• 5(6)-Carboxyfluorescein
• Fluorescein diacetate
• 5(6)-CFDA
• 5-CARBOXYFLUORESCEIN DIACETATE
• 4-(3-ACETOXYPHENYL)-1-BUTENE
• 4-(2,5-DIMETHOXYPHENYL)-1-BUTENE
• ACRYLOYL-X, SE
• Nhs Ester