6-CARBOXYFLUORESCEIN DIACETATE Chemical Properties

Melting point:

152–153°C

Boiling point:

701.6±60.0 °C(Predicted)

Density 

1.57±0.1 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

DMSO: soluble

pka

6.4(at 25℃)

form 

Solid

color 

Light yellow or off-white solid

PH Range

Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)

λmax

<300nm

Major Application

Fluorescent probes, detecting cells, microorganisms, yeasts, antifungal agent, antibacterials agent

Biological Applications

Analyzing/detecting cells;counting/ detecting/evaluating microorganisms;detecting/ measuring bacteria;quantifying adhesion of tumor cells to endothelial monolayers; measuring gas (carbon dioxide or ammonia) concentration in body luids; monitoring intracellular nitric oxide production;screening xenobiotic phloem mobility in plants; , studying low-density lipoprotein (LDL)/cholesterol metabolism;apoptosis assay;method for antifungal susceptibility testing; , as a substrate for measuring esterase activity

CAS DataBase Reference

3348-03-6(CAS DataBase Reference)

Safety Information

WGK Germany 

3

F 

10-21

HS Code 

29322090

MSDS

• Language:English Provider:SigmaAldrich

6-CARBOXYFLUORESCEIN DIACETATE Usage And Synthesis

Description

6-Carboxyfluorescein diacetate is a membrane-permeant prodrug of 6-Carboxyfluorescein, which has been used to differentiate viable cells from apoptotic cells.

Uses

6-Carboxyfluorescein Diacetate is a fluorescent polyanionic probe used to detect changes intracellular pH. In addition, 6-Carboxyfluorescein Diacetate derivatives can be used as staining agents for flow cytometry to visualize cells undergoing apoptosis or necrosis.

Uses

6-CARBOXYFLUORESCEIN DIACETATE is used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin

Excitation / Emission Maximum

553 nm/575 nm

6-CARBOXYFLUORESCEIN DIACETATE(3348-03-6)Related Product Information

• 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
• 6-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester
• 5-CFDA N-succinimidyl ester
• 6-CARBOXYFLUORESCEIN DIACETATE
• 6-CARBOXYFLUORESCEIN DIPIVALATE
• 5(6)-CFDA
• 5-CARBOXYFLUORESCEIN DIACETATE
• 6-Carboxyfluorescein
• Fluorescein diacetate
• 5(6)-CARBOXYEOSIN DIACETATE N-HYDROXY-SUCCINIMIDE ESTER
• 6-Carboxy-2',7'-dichlorofluorescein 3',6'-Diacetate SucciniMidyl Ester
• 5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE, SUCCINIMIDYL ESTER
• 5(6)-CARBOXYEOSIN DIACETATE
• 5-CARBOXYFLUORESCEIN DIACETATE, ACETOXYMETHYL ESTER,5-Carboxyfluorescein diacetate acetoxylmethyl ester
• 5,6-CARBOXYFLUORESCEIN DIPIVALATE SUCCINIMIDE ESTER
• 5(6)-CFDA [5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE] *MIXED ISOMERS*
• 6-CARBOXYFLUORESCEIN DIACETATE (6-CFDA)
• 5-(AND-6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE