5-CARBOXYFLUORESCEIN DIACETATE Chemical Properties

Melting point:

>250°C

Boiling point:

693.1±55.0 °C(Predicted)

Density 

1.57±0.1 g/cm3(Predicted)

storage temp. 

Sealed in dry,Store in freezer, under -20°C

solubility 

DMSO: soluble

pka

6.4(at 25℃)

form 

Solid

color 

Colorless solid

PH Range

Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)

λmax

<300nm

BRN 

68265

Major Application

Sensors, method for measuring bacteria, detecting intracellular pH inyeast, determination of extracellular and intracellular pH of microorganisms, detecting living cells, identification of cancerous cells, method for assessing microorganism viability, proliferative activities in tumor tissues, measuring the growth of keratinocytes, agent, neurodegenerative disorders, antineoplastic drugs

Biological Applications

Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity

Safety Information

Hazard Codes 

T

Risk Statements 

45-46-23/24/25-36/37/38

Safety Statements 

53-22-26-36/37/39-45

WGK Germany 

3

F 

10-21

HS Code 

32049010

5-CARBOXYFLUORESCEIN DIACETATE Usage And Synthesis

Uses

Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein.

Uses

5-Carboxyfluorescein Diacetate is used in the synthesis of Rhodamines. Dyes and metabolites.

5-CARBOXYFLUORESCEIN DIACETATE(79955-27-4)Related Product Information

• 5(6)-CFDA
• 5-CARBOXYFLUORESCEIN DIACETATE
• 5-CFDA N-succinimidyl ester
• BCECF-AM
• 5(6)-CARBOXYFLUORESCEIN DIISOBUTYRATE
• 6-CARBOXYFLUORESCEIN DIACETATE
• 6-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester
• 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
• 6-Carboxyfluorescein
• Fluorescein diacetate
• 5(6)-Carboxyfluorescein
• 5(6)-CARBOXYEOSIN DIACETATE
• 5(6)-CARBOXYEOSIN DIACETATE N-HYDROXY-SUCCINIMIDE ESTER
• 5-CARBOXYFLUORESCEIN DIACETATE, ACETOXYMETHYL ESTER,5-Carboxyfluorescein diacetate acetoxylmethyl ester
• 5-CARBOXYFLUORESCEIN DIPIVALATE N-HYDROXYSUCCINIMIDE ESTER
• 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER
• 5,6-CARBOXYFLUORESCEIN DIPIVALATE
• 5-(and-6)-carboxy-2`,7`-dichlorofluorescein diacetate