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5-METHYLCYTOSINE

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5-METHYLCYTOSINE Basic information

Product Name:
5-METHYLCYTOSINE
Synonyms:
  • 4-Amino-5-methyl-2-pyrimidinol
  • Cytosine, 5-methyl-
  • 5-Methylcytosine,>96%
  • 2(1H)-Pyrimidinone, 4-amino-5-methyl- (9CI)
  • 4-amino-5-methyl-3H-pyrimidin-2-one
  • 5-METHYLCYTOSINE
  • 2(1H)-Pyrimidinone, 6-amino-5-methyl-
  • 2(1H)-Oxo-4(3H)-imino-5-methylpyrimidine
CAS:
554-01-8
MF:
C5H7N3O
MW:
125.13
EINECS:
209-058-3
Product Categories:
  • Nucleic acids
  • Pyrimidines
  • PYRIMIDINE
  • Elisa Kit-Rat Elisa Kit
Mol File:
554-01-8.mol
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5-METHYLCYTOSINE Chemical Properties

Melting point:
270°C (rough estimate)
Boiling point:
232.57°C (rough estimate)
Density 
1.2602 (rough estimate)
refractive index 
1.6510 (estimate)
storage temp. 
Keep in dark place,Inert atmosphere,Room temperature
solubility 
Methanol (Sparingly, Heated)
pka
9.05±0.10(Predicted)
form 
Crystalline
color 
White to Off-White
Water Solubility 
Soluble in water.
Merck 
14,6050
CAS DataBase Reference
554-01-8(CAS DataBase Reference)
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Safety Information

RTECS 
UW7362350

MSDS

  • Language:English Provider:ALFA
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5-METHYLCYTOSINE Usage And Synthesis

Chemical Properties

Crystals in prisms from water.

Uses

5-Methylcytosine is used as a marker to protect DNA from being cut by native methylation-sensitive restriction enzymes. It is also employed in the production of 4-amino-5-hydroxy-5-methyl-2-oxo-2,5-dihydro-imidazole-1-carbaldehyde.

Definition

ChEBI: A pyrimidine that is a derivative of cytosine, having a methyl group at the 5-position.

Purification Methods

Crystallise it from water (solubility is 3.4%). The hydrochloride has m 299-301o (sintering at 280o) (from aqueous HCl/Me2CO). [Hitchings et al. J Biol Chem 177 537 1949, Cohn J Am Chem Soc 73 1539 1951, Beilstein 25 II 183, 25 III/IV 3727.]

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