1,10-Phenanthroline hydrate Chemical Properties

Melting point:

100-104 °C(lit.)

Density 

1.10

vapor density 

6.2 (vs air)

storage temp. 

Sealed in dry,Room Temperature

solubility 

methanol: soluble200MM (Stock solution is stable for months at –20° C.)

form 

Needle-Like Crystals

color 

Off-white, may become cream-colored during storage

Water Solubility 

<0.01 g/100 mL at 21 ºC

Merck 

14,7214

BRN 

4008096

Stability:

Stable. Incompatible with iron, other heavy metals, strong oxidizing agents, strong acids.

CAS DataBase Reference

5144-89-8(CAS DataBase Reference)

EPA Substance Registry System

1,10-Phenanthroline, monohydrate (5144-89-8)

Safety Information

Hazard Codes 

T,N

Risk Statements 

25-50/53

Safety Statements 

45-60-61

RIDADR 

UN 2811 6.1/PG 3

WGK Germany 

3

RTECS 

SF8437000

TSCA 

Yes

HazardClass 

6.1

PackingGroup 

III

HS Code 

29339990

Toxicity

LD50 orally in Rabbit: 132 mg/kg

MSDS

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1,10-Phenanthroline hydrate Usage And Synthesis

Chemical Properties

white crystals or powder

Uses

1,10-Phenanthroline monohydrate is useful to study the DNA-protein interactions due to its nuclease activity. It is used as a redox indicator and reagent for spectrophotometric assay of silver. It acts as a chelating ligand for the determination of Fe, Pd and V. It also acts as a matrix metalloproteinase inhibitor. It is a photometric reagent for iron, which forms a red chelate with ferrous (iron II) ion.

Uses

When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.
1,10-Phenanthroline monohydrate is an aromatic nitrogen heterocycle that may be used as a chelating agent in the preparation of metal-chelate complexes.

General Description

White crystalline powder or solid. Slight odor.

Air & Water Reactions

Insoluble in water.

Reactivity Profile

1,10-Phenanthroline hydrate is incompatible with strong oxidizers. 1,10-Phenanthroline hydrate is also incompatible with strong acids. 1,10-Phenanthroline hydrate forms complex compounds with ferrous ions. .

Fire Hazard

Flash point data for 1,10-Phenanthroline hydrate are not available. 1,10-Phenanthroline hydrate is probably combustible.

Biochem/physiol Actions

Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.

Purification Methods

Crystallise its picrate (m 191o) from EtOH; then the free base is liberated with aqueous alkali, dried at 78o/8mm over P2O5 and crystallised from pet ether (b 80-100o). [Cumper et al. J Chem Soc 1188 1962.] It can be purified by zone melting. It has also been crystallised from hexane, *benzene/pet ether (b 40-60o) or sodium-dried *benzene, dried and stored over H2SO4. The monohydrate is obtained by crystallisation from aqueous EtOH or ethyl acetate. It has been crystallised from H2O (300 parts) to give the monohydrate m 102-103o which sublimes at 10-3mm [Fielding & LeFevre J Chem Soc 1811 1951.] The anhydrous compound has m 118o (after drying at high vacuum at 80o) and is also obtained by recrystallisation from pet ether or *C6H6 (70 parts) and drying at 78o/8mm. [UV: Badger et al. J Chem Soc 3199 1951.] IthasapKainH2Oof 4.857 (25o) or 5.02 (20o) and 4.27 in 50% aqueous EtOH (20o). [Albert et al. J Chem Soc 2240 1948]. [Beilstein 23 H 227, 23 II 235, 23/8 V 419.]

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