ChemicalBook > CAS DataBase List > Creatine Phosphokinase

Creatine Phosphokinase

Product Name
Creatine Phosphokinase
CAS No.
9001-15-4
Chemical Name
Creatine Phosphokinase
Synonyms
CK;CPK;PCPK;RCPK;FY032;Mib-CK;S-MtCK;EC 2.7.3.2;EC: 2.7.3.2;CREATINE KINASE
CBNumber
CB0750174
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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Creatine Phosphokinase Property

storage temp. 
-20°C
solubility 
0.25 M glycyl-glycine, pH 7.4: soluble5.0mg/mL, clear, colorless to slightly yellow
form 
salt-free, lyophilized powder
color 
white
biological source
rabbit skeletal muscle
Water Solubility 
aqueous buffer: soluble
water: soluble
Specific Activity
≥30units/mg protein
CAS DataBase Reference
9001-15-4
EPA Substance Registry System
Kinase (phosphorylating), creatine (9001-15-4)
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Safety

WGK Germany 
3
10-21
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
C7886
Product name
Creatine Phosphokinase from bovine heart
Purity
Type III, salt-free, lyophilized powder, ≥30?units/mg protein
Packaging
500units
Price
$71.8
Updated
2025/07/31
Sigma-Aldrich
Product number
C7886
Product name
Creatine Phosphokinase from bovine heart
Purity
Type III, salt-free, lyophilized powder, ≥30?units/mg protein
Packaging
1000units
Price
$112
Updated
2025/07/31
Sigma-Aldrich
Product number
C7886
Product name
Creatine Phosphokinase from bovine heart
Purity
Type III, salt-free, lyophilized powder, ≥30?units/mg protein
Packaging
3500units
Price
$288
Updated
2025/07/31
Sigma-Aldrich
Product number
C3755
Product name
Creatine Phosphokinase from rabbit muscle
Purity
Type I, salt-free, lyophilized powder, ≥150?units/mg protein
Packaging
500units
Price
$63.4
Updated
2025/07/31
Sigma-Aldrich
Product number
C7886
Product name
Creatine Phosphokinase from bovine heart
Purity
Type III, salt-free, lyophilized powder, ≥30?units/mg protein
Packaging
17500units
Price
$1080
Updated
2025/07/31
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Creatine Phosphokinase Chemical Properties,Usage,Production

Chemical Properties

Off-white salt-free lyophilized powder, soluble in water, optimal pH 6.0-7.0 (positive reaction), 9.0 (negative reaction). Stability: inactivated at pH less than 5, thawing and freezing also inactivate the enzyme; -20℃, pH 6.6, in 0.1mot/L imidazole buffer can be stabilized for 4 weeks, neutral 60% ethanol solution is the most stable, dilute solution by adding 0.1% albumin or β-mercaptoethanol can increase the stability; freeze-dried products can be stored at -20℃ for 12 months, at 4℃ for 6 months. The freeze-dried product can be stored at -20℃ for 12 months, and at 4℃ for 6 months, it loses 15% of its vitality. Inhibitors include p-chloromercuric acid benzoic acid, chelating agent, iodoacetic acid, tripolyphosphoric acid, adenosine, nitrite, sulfate, bromide, sulfite, chloride, fluoride thyroxine, 3,5-dinitro-o-cresol, coenzyme I (NAD), L-triiodocarbonylammonium (T3), malonate, iodine (I-), iodoacetic acid, orthophosphate, pyrophosphate, and triple phosphoric acid, etc., and activators include Mg2+, Mn2+, 2-mercaptoethanol, dithiothreitol, glutathione, thiol N-acetylcysteine, etc. Absorbance coefficient of 1% aqueous solution at 280 nm was 8.76. Enzymatic reaction: creatine + adenosine triphosphate ═adenosine diphosphate + phosphocreatine.

Uses

The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.

Uses

The enzyme from Sigma has been used in the measurement of phosphocreatine in hippocampal neurons isolated from rat brains.

Biochem/physiol Actions

Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.

Creatine Phosphokinase Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Creatine Phosphokinase manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Creatine Phosphokinase 9001-15-4
Price
US $0.00-0.00/g
Min. Order
1g
Purity
98.0%
Supply Ability
1kg/month
Release date
2021-08-27
Hangzhou ICH Biofarm Co., Ltd
Product
Creatine Phosphokinase 9001-15-4
Price
US $0.00-0.00/g
Min. Order
1g
Purity
99% HPLC
Supply Ability
100kg
Release date
2023-06-30

9001-15-4, Creatine PhosphokinaseRelated Search:


  • AMP phosphotransferase
  • Creatine phosphokinase, from rabbit muscle, typeI, >=150 units/mg
  • ATP: CREATINE N-PHOSPHOTRANSFERASE
  • ATP: CREATINE N-PHOSPHOTRANSFERASE TYPE I
  • ATP: CREATINE N-PHOSPHOTRANSFERASE TYPE III
  • ATP: CREATINE N-PHOSPHOTRANSFERASE TYPE IV
  • CK
  • CPK
  • CREATINE KINASE TYPE I
  • CREATINE KINASE TYPE III
  • CREATINE KINASE TYPE IV
  • CREATINE PHOSPHOKINASE, PORCINE
  • CREATINE PHOSPHOKINASE, RABBIT
  • CREATINE PHOSPHOKINASE, RABBIT SKELETAL MUSCLE
  • CREATINE PHOSPHOKINASE TYPE I
  • CREATINE PHOSPHOKINASE TYPE III
  • CREATINE PHOSPHOKINASE TYPE IV
  • CREATINE KINASE
  • CREATINE PHOSPHOKINASE
  • CREATINE PHOSPHOKINASE FROM RABBIT MUSCL E, ~14 U/MG
  • CREATINE PHOSPHOKINASE TYPE IV FROMRABBI T BRAIN
  • CREATINE PHOSPHOKINASE FROM RABBIT MUSCL E, ~20 U/MG
  • 78861, Creatine kinase (CK-BB iso-enzyme ) (catalytic concentration)
  • CREATINE PHOSPHOKINASE TYPE I FROMRABBIT MUSCLE
  • CREATINE PHOSPHOKINASE TYPE III FROMBOVI NE HEART
  • CreatineKinaseExRabbitMuscle
  • CPK:frombovineheartorrabbitmuscle
  • Creatine kinase from rabbit muscle ca. 240 U/mg
  • creatine phosphokinase from bovine heart
  • creatine phosphokinase from rabbit brain
  • CREATINE KINASE, FROM BOVINE HEART
  • Creatine kinase-2
  • Creatine kinase-2 (CK-MB isoenzyme) (catalytic concentration)
  • ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
  • CK diagnostic kit.
  • creatine phosphokinase(rabbit music)
  • reatine phosphokinase (rabbit music)
  • EC 2.7.3.2
  • EC: 2.7.3.2
  • PCPK
  • PHOSPHOCREATINE PHOSPHOKINASE
  • PHOSPHOCREATINE PHOSPHOKINASE TYPE I
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  • CREATINE KIN. FR. RABBIT MUSC.CA.240 U/MG LYOPH. SALT-FREE
  • Creatine Phosphokinase from rabbit muscle,ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
  • Creatine Phosphokinase from rabbit brain,ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
  • Creatine Phosphokinase from bovine heart,ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
  • Basic-type mitochondrial creatine kinase
  • Mib-CK
  • sarcomeric mitochondrial
  • S-MtCK
  • Adenylate kinase isoenzyme 1
  • ATP-AMP transphosphorylase 1
  • PHOSPHOCREATINE PHOSPHOKINASE TYPE III
  • PHOSPHOCREATINE PHOSPHOKINASE TYPE IV
  • RCPK
  • Creatine kinase (CK-BB iso-enzyme) (catalytic concentration)