6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
- Product Name
- 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
- CAS No.
- 150810-68-7
- Chemical Name
- 6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER
- Synonyms
- CS-2126;5-TAMRASE;5-TAMRA,SE, >95%;5-TAMRA-SEor5-TAMRA-NHS;5-TAMRA SE (Orange Red);5-TAMRA Azide (CCT-1245);5-TAMRA-SE, 10 mM in DMSO;5-TAMRA N-Succinimidyl Ester;5-TAMRA,SE [5-Carboxytetramethylrhodamine, SE];5-Carboxytetramethylrhodamine succinimidyl ester
- CBNumber
- CB1207615
- Molecular Formula
- C29H25N3O7
- Formula Weight
- 527.53
- MOL File
- 150810-68-7.mol
6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER Property
- storage temp.
- Keep in dark place,Inert atmosphere,Store in freezer, under -20°C
- solubility
- DMF: soluble
- form
- A solid
- color
- Pale purple to purple
- Appearance
- Dark red amorphous solid
- InChIKey
- VWFRSNKRTNUMET-UHFFFAOYSA-N
- SMILES
- C(C1C=C(C(=O)ON2C(CCC2=O)=O)C=CC=1C1=C2C=CC(N(C)C)=CC2=[O+]C2C=C(N(C)C)C=CC1=2)(=O)[O-]
Safety
- WGK Germany
- 3
- HS Code
- 32049010
N-Bromosuccinimide Price
- Product number
- 53048
- Product name
- 5-Carboxy-tetramethylrhodamine N-succinimidyl ester
- Purity
- BioReagent,suitableforfluorescence
- Packaging
- 1mg
- Price
- $169
- Updated
- 2025/07/31
- Product number
- 53048
- Product name
- 5-Carboxy-tetramethylrhodamine N-succinimidyl ester
- Purity
- BioReagent,suitableforfluorescence
- Packaging
- 5mg
- Price
- $573
- Updated
- 2025/07/31
- Product number
- T2808
- Product name
- 5-TAMRA SE
- Packaging
- 5MG
- Price
- $167
- Updated
- 2025/07/31
- Product number
- T2808
- Product name
- 5-TAMRA SE
- Packaging
- 25MG
- Price
- $499
- Updated
- 2025/07/31
- Product number
- 28509
- Product name
- 5-Carboxytetramethylrhodamine succinimidyl ester
- Packaging
- 5mg
- Price
- $116
- Updated
- 2024/03/01
6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER Chemical Properties,Usage,Production
Description
5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe. It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin , nucleotide diphosphate uridine-5’-diphosphate (UDP; ), and the progesterone receptor antagonist RU486 (mifepristone; ). 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.
Uses
Amine-reactive form of 5-carboxytetramethylrhodamine single isomer.
Uses
Amine-Reactive Fluorescent Probe for labeling of DNA and RNA
storage
Store at -20°C
References
[1] KIERAN F. GEOGHEGAN. Site-directed double fluorescent tagging of human renin and collagenase (MMP-1) substrate peptides using the periodate oxidation of N-terminal serine. An apparently general strategy for provision of energy-transfer substrates for proteases[J]. Bioconjugate Chemistry Bioconjugate, 1993, 4 6: 537-544. DOI: 10.1021/bc00024a017
[2] ADAM B. SHAPIRO* Mark S Janelle Comita Prevoir. 5-Carboxytetramethylrhodamine-Ampicillin Fluorescence Anisotropy-Based Assay of Escherichia coli Penicillin-Binding Protein 2 Transpeptidase Inhibition[J]. ACS Infectious Diseases, 2019, 5 6: 863-872. DOI: 10.1021/acsinfecdis.8b00351
[3] JUN QI Pablo S Michelle Oppenheimer. Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase.[J]. Enzyme Research, 2011, 2011: 513905. DOI: 10.4061/2011/513905
[4] ROY WEINSTAIN. Fluorescent Ligand for Human Progesterone Receptor Imaging in Live Cells[J]. Bioconjugate Chemistry Bioconjugate, 2013, 24 5: 766-771. DOI: 10.1021/bc3006418
Abs/Em Maxima
553/575 nm
Excitation / Emission Maximum
546 nm/580 nm
Extinction Coefficient
92,000
Microscopy Laser Line
532 nm or 555 nm
Flow Cytometry Laser Line
532 nm or 555 nm
Spectrally Similar Dyes
Alexa Fluor® 555, CF® 555, DyLight® 549
6-CARBOXYTETRAMETHYLRHODAMINE N-HYDROXYSUCCINIMIDE ESTER Preparation Products And Raw materials
Raw materials
Preparation Products
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