ChemicalBook > CAS DataBase List > Proteinase K

Proteinase K

Product Name
Proteinase K
CAS No.
39450-01-6
Chemical Name
Proteinase K
Synonyms
Protease K;Tritirachium album proteinase K;20mg;bum;PROK;40U/MG;CHLOROM;C:C&B53;abs9265;abs9118
CBNumber
CB1399741
Molecular Formula
C29H27N2O12P
Formula Weight
626.504641
MOL File
39450-01-6.mol
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Proteinase K Property

Density 
1.1g/cm3
bulk density
35kg/m3
storage temp. 
2-8°C
solubility 
H2O: 10 mg/mL, slightly hazy, brown-yellow
form 
powder
color 
white
PH
pH7.5～12
biological source
microbial (T.album
T. ALBUM)
Water Solubility 
Miscible with water.
Specific Activity
30units/mg protein
InChI
InChI=1/C29H27N2O12P/c1-18(32)23-26(33)30-24(27(34)39-3)25(40-20-10-6-4-7-11-20)28(2,41-21-12-8-5-9-13-21)29(23,30)43-44(37,38)42-22-16-14-19(15-17-22)31(35)36/h4-18,23,32H,1-3H3,(H,37,38)/t18-,23-,28-,29+/s3
InChIKey
CDWGKFQEIBBJOF-BIZWTRPUNA-N
SMILES
N12[C@](OP(O)(OC3=CC=C([N+]([O-])=O)C=C3)=O)([C@H]([C@H](O)C)C1=O)[C@](C)(OC1=CC=CC=C1)C(OC1=CC=CC=C1)=C2C(OC)=O |&1:1,16,17,22,r|
CAS DataBase Reference
39450-01-6
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Safety

Hazard Codes 
Xn
Risk Statements 
36/37/38-42
Safety Statements 
23-24-26-36/37-22-45
WGK Germany 
1
F 
10
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
70663
Product name
Proteinase K, Lyophilized
Purity
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.
Packaging
100mg
Price
$155
Updated
2024/03/01
Sigma-Aldrich
Product number
70663
Product name
Proteinase K, Lyophilized
Purity
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.
Packaging
500mg
Price
$506
Updated
2024/03/01
Sigma-Aldrich
Product number
539480
Product name
Proteinase K from Tritirachium album
Packaging
25MG
Price
$58
Updated
2024/03/01
Sigma-Aldrich
Product number
539480
Product name
Proteinase K from Tritirachium album
Packaging
100MG
Price
$132
Updated
2024/03/01
Alfa Aesar
Product number
J62051
Product name
Proteinase K, Tritirachium album limber
Packaging
100mg
Price
$109.2
Updated
2021/12/16
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Proteinase K Chemical Properties,Usage,Production

Description

Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in the absence of this divalent cation some catalytic activity is retained. Maximum proteinase K activity is observed with the inclusion of 1 mM Ca++ in the reaction buffer. Proteinase K digestion is routinely performed at 50°C, and occasionally in the presence of EDTA to inhibit labile, Mg++-dependent nucleases. Proteinase K is prepared commonly as a 20 mg/mL stock solution in sterile water (stable for 1 year at ?20°C) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl2 (stable for months at 4°C). It is generally used at a working concentration of up to 50 μg/mL in any of a number of buffer formulations, including those that contain as much as 0.5% SDS[1].

Chemical Properties

Powder beige

Uses

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

Uses

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Definition

Proteinase K cleaves peptide bonds next to the carboxyl-terminal of aromatic amino acids, hydrophobic amino acids and sulfuric amino acids, within the polypeptide chain. Hence Proteinase K can act as an endoprotease on a wide range of protein substrates. Because of its proteolytic preferences, it is a member of the subtilisin group of serine proteases. The K in Proteinase K refers to its ability to digest Keratin protein found in hairs, nails and hooves. Proteinase K is an essential reagent in molecular biology labs because it is useful in isolating DNA and RNA molecules by degrading and inactivating proteins. Fungus Tritirachium album Limber naturally produces proteinase K.

General Description

Proteinase K, an extracellular endopeptidase is synthesized by the mold, Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein and is characterized with an unhydrolyzed protein chain and autolyzed polypeptide chains.

Biochem/physiol Actions

Proteinase K catalyzes the hydrolysis of keratin.

Mechanism of action

Proteinase K is a serine protease that cleaves peptide bonds in proteins. Its mechanism of action involves several steps:
Binding: Proteinase K first binds to the protein or nucleic acid substrate through non-specific hydrophobic interactions.
Activation: Once bound, the enzyme undergoes an activation step where a catalytic serine residue is activated by a histidine residue and a water molecule. This leads to the forming of an active site that can cleave peptide bonds.
Cleavage: The active site of proteinase K cleaves the peptide bond on the carboxylic acid side of the amino acid residues that contain aromatic, aliphatic or hydrophobic amino acid residues. The enzyme can also cleave peptide bonds on the amide side of glycine residues.
Product release: After cleavage, the products of the reaction (i.e., peptides) are released from the enzyme.
The mechanism of action of proteinase K is similar to other serine proteases. Still, it is notable for its ability to function under harsh conditions, such as high temperatures and in the presence of detergents.

References

[1] Farrell, R. “Creating a Ribonuclease-Free Environment.”RNA Methodologies (Sixth Edition) 2023: 51-70.

Proteinase K Preparation Products And Raw materials

Raw materials

Preparation Products

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Proteinase K Suppliers

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View Lastest Price from Proteinase K manufacturers

Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Proteinase K 39450-01-6
Price
US $99.00-35.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20ton
Release date
2024-05-08
Hebei Saisier Technology Co., LTD
Product
Proteinase K 39450-01-6
Price
US $6.00/KG
Min. Order
1KG
Purity
More than 99%
Supply Ability
2000KG/Month
Release date
2024-04-23
Hebei Mujin Biotechnology Co.,Ltd
Product
Proteinase K 39450-01-6
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-11-13

39450-01-6, Proteinase KRelated Search:

Papain Trypsin EC 3.4.21.19 Pepsin Chymotrypsin ENTEROKINASE Aprotinin Proteinase K Solution DISPASE I EC 3.4.21.14,PROTEINASE 6 FROM ASPERGILLUS SP., ~3 UN ITS/MG EC 3.4.21.14,PROTEINASE, BACTERIAL, ~10 U/MG Proteinase, Bacillus subtilis metallo- Acid resistant proteinase neutral proteinase PROTEINASE FROM ASPERGILLUS NIGER >4 U/M,EC 3.4.21.14 EC 3.4.21.14,EC 3.4.21.64 EC 3.4.21.14 Proteinase, Bacillus subtilis, sutilain

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