CARBOXYPEPTIDASE A

ChemicalBook > CAS DataBase List > CARBOXYPEPTIDASE A

Product Name: CARBOXYPEPTIDASE A
CAS No.: 11075-17-5
Chemical Name: CARBOXYPEPTIDASE A
Synonyms: EC 3.4.17.1;E.C. 3.4.2.1;E.C. 3.4.12.2;CARBOXYPEPTIDASE A;CARBOXYPOLYPEPTIDASE;Carboxypeptidase A?Agarose;PEPTIDYL-L-AMINO-ACID HYDROLASE;CARBOXYPEPTIDASE A TYPE II-PMSF;CARBOXYPEPTIDASE A, PMSF TREATED;CarboxypeptidaseAExBovinePanceas
CBNumber: CB1776427
Molecular Formula: NULL
Formula Weight: 0
MOL File: Mol file

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CARBOXYPEPTIDASE A Property

Density: 1.00 g/mL at 20 °C
Flash point:: 4 °C
storage temp.: 2-8°C
form: suspension
color: white
biological source: enzyme from bovine pancreas
Specific Activity: ≥6units/mL packed gel, 25°C
CAS DataBase Reference: 11075-17-5

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Safety

Hazard Codes: F,Xn,B,Xi
Risk Statements: 11-38-42-48/20-63-65-67-36-36/37/38
Safety Statements: 36/37-45-62-36-26-23-16-24-22
RIDADR: UN 1294 3/PG 2
WGK Germany: 2
F: 10

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: SAE0046
Product name: Carboxypeptidase A from bovine pancreas
Purity: (Type II-PMSF treated), ≥20?units/mg protein
Packaging: 500units
Price: $46.8
Updated: 2024/03/01

Sigma-Aldrich

Product number: SAE0046
Product name: Carboxypeptidase A from bovine pancreas
Purity: (Type II-PMSF treated), ≥20?units/mg protein
Packaging: 2500Units
Price: $133
Updated: 2024/03/01

Sigma-Aldrich

Product number: SAE0046
Product name: Carboxypeptidase A from bovine pancreas
Purity: (Type II-PMSF treated), ≥20?units/mg protein
Packaging: 5000units
Price: $235
Updated: 2024/03/01

Sigma-Aldrich

Product number: C9268
Product name: Carboxypeptidase A from bovine pancreas
Purity: (Type II-PMSF treated), ≥50?units/mg protein, aqueous suspension
Packaging: 500units
Price: $143
Updated: 2024/03/01

Sigma-Aldrich

Product number: C9268
Product name: Carboxypeptidase A from bovine pancreas
Purity: (Type II-PMSF treated), ≥50?units/mg protein, aqueous suspension
Packaging: 2500Units
Price: $270
Updated: 2024/03/01

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CARBOXYPEPTIDASE A Chemical Properties,Usage,Production

Chemical Properties

Suspension (white)

Uses

The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.

Definition

An enzyme that catalyzes the cleavage of amino acids from a protein at the C-terminus of a polypeptide chain.

General Description

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Industrial uses

Carboxypeptidase A(CPA) is an enzyme of the digestive system that is known to cleave amino acids favouring the C-terminal end as well as certain esters. This enzymatic activity depends on the metal at the catalytic site. Zn²⁺ and some Co²⁺-containing CPAs exhibit peptidase function, whilst esterase function has been seen by CPAs containing a variety of divalent d-block metals. CPA has a size similar to CA, consisting of about 300 amino acids and a molecular mass of 34 kDa.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.

Purification Methods

Carboxypeptidase A is purified by DEAE-cellulose chromatography, activation with trypsin and dialysed against 0.1M NaCl, yielding crystals. It is recrystallised by dissolving in 20 mL of M NaCl and dialysed for 24hours each against the following salts present in 500mL of 0.02M sodium veronal pH 8.0, 0.5M NaCl, 0.2M NaCl and 0.15M NaCl. The last dialysate usually induces crystallisation. If it does not crystallise, then dialyse the last solution against 0.02M sodium veronal containing 0.10M NaCl. Only 2 or 3 recrystallisations are required to attain maximum activity. [Cox et al. Biochemistry 3 44 1964.] Enzyme activity is measured by hydrolysing hippuryl-L-phenylalanine (or phenylacetic acid) and observing the rate of change of optical density at 254nm (reaction extinction coefficient is ~0.592 cm2/Wmole at pH 7.5) [Bergmyer Methods in Enzymatic Analysis (Academic Press) 1 436 1974].

CARBOXYPEPTIDASE A Preparation Products And Raw materials

Raw materials

Preparation Products

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11075-17-5, CARBOXYPEPTIDASE ARelated Search:

TERT-BUTYL ISOCYANIDE Cupric acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE SALCOMINE PHENYLSELENOL N-BUTYLISOCYANIDE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Tosylmethyl isocyanide Ferric acetylacetonate METHYL ISOCYANOACETATE Aluminum acetylacetonate COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Ethyl isocyanoacetate Tris(2,4-pentanedionato)chroMiuM(III) Benzyl isocyanide CARBOXYPEPTIDASE B

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase from bovine pancreas

Carboxypeptidase A from bovine pancreas,Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

Peptidyl-L-amino-acid hydrolase from bovine pancreas

Carboxypeptidase A?Agarose

E.C. 3.4.12.2

E.C. 3.4.2.1

PEPTIDYL-L-AMINO-ACID HYDROLASE

PEPTIDYL-L-AMINO-ACID HYDROLASE TYPE II-PMSF

EC 3.4.17.1

CARBOXYPOLYPEPTIDASE

CARBOXYPOLYPEPTIDASE TYPE II-PMSF

CARBOXYPEPTIDASE A

CARBOXYPEPTIDASE A, BOVINE PANCREAS

CARBOXYPEPTIDASE A, PMSF TREATED

CARBOXYPEPTIDASE A TYPE II-PMSF

carboxypeptidase A from bovine pancreas stab.

carboxypeptidase A type I from*bovine pancreas

carboxypeptidase A-pmsf from*bovine pancreas type

CARBOXYPEPTIDASE A FROM BOVINE PANCREAS STAB., ~70 U/MG*

CARBOXYPEPTIDASE A FROM HUMAN PANCREAS

CARBOXYPEPTIDASE A FROM BOVINE PANCREAS, ~1100 U/ML

CARBOXYPEPTIDASE A TYPE II2X CRYSTALLIZE D

CARBOXYPEPTIDASE A-PMSF FROMBOVINE PANCR EAS TYPE I

CarboxypeptidaseAExBovinePanceas

carboxypeptidaseaphenylmethylsulfonylfluoridetreated

carboxypeptidase a from bovine pancreas

Carboxypeptidase A, PMSF treated from bovine pancreas from bovine pancreas

Carboxypeptidase A from bovine pancreas [EC 3.4.17.1] from bovine pancreas

Peptidyl-L-amino-acid hydrolase from human pancreas

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

Native Bovine Carboxypeptidase A

Carboxypeptidase A from bovine pancreas (Type II-PMSF treated), >=50 units/mg protein, aqueous suspension

11075-17-5

BioChemical

Enzymes, Inhibitors, and Substrates

Proteolytic Enzymes and Substrates

Proteolytic Enzymes

Biochemicals and Reagents