ChemicalBook > CAS DataBase List > DAF-FM DA

DAF-FM DA

Product Name
DAF-FM DA
CAS No.
254109-22-3
Chemical Name
DAF-FM DA
Synonyms
DAF-FM DA;DAF-FM DA solution;-[9H]xanthen]-3-one;DAF-FM DA (cell permeable);DAF-FM DA [DAF-FM Diacetate];-difluoro-spiro[isobenzofuran-1(3H);Diaminofluorescein-FM diacetate;DAF-FM Diacetate NO fluorescent probe;DAF-FM DA - CAS 254109-22-3 - Calbiochem;4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy)
CBNumber
CB21074275
Molecular Formula
C25H18F2N2O7
Formula Weight
496.42
MOL File
254109-22-3.mol
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DAF-FM DA Property

Boiling point:
695.7±55.0 °C(Predicted)
Density 
1.56±0.1 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
Soluble in dimethyl sulfoxide
pka
4.20 ± 0.20, most basic, temperature: 25 °C
form 
Pale yellow solution
color 
clear clear, colorless
λmax
365 nm (MeOH)
Biological Applications
Detecting bacteria/microorganisms; nitric oxide indicator,
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
D1946
Product name
DAF-FM DA
Purity
≥98% (HPLC)
Packaging
1mg
Price
$737
Updated
2024/03/01
Sigma-Aldrich
Product number
251520-M
Product name
DAF-FM DA - CAS 254109-22-3 - Calbiochem
Purity
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
Packaging
251520-1MG
Price
$1010
Updated
2024/03/01
Sigma-Aldrich
Product number
251520-M
Product name
DAF-FM DA - CAS 254109-22-3 - Calbiochem
Purity
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
Packaging
1mg
Price
$957
Updated
2023/01/07
Cayman Chemical
Product number
18767
Product name
DAF-FM diacetate
Purity
≥98%
Packaging
1mg
Price
$413
Updated
2024/03/01
Cayman Chemical
Product number
18767
Product name
DAF-FM diacetate
Packaging
5mg
Price
$1543
Updated
2024/03/01
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DAF-FM DA Chemical Properties,Usage,Production

Uses

DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495

Uses

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Biochem/physiol Actions

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

storage

Store at -20°C

DAF-FM DA Preparation Products And Raw materials

Raw materials

Preparation Products

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DAF-FM DA Suppliers

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254109-22-3, DAF-FM DARelated Search:

SALCOMINE Tris(2,4-pentanedionato)chroMiuM(III) PHENYLSELENOL 2,4-PENTANEDIONE, SILVER DERIVATIVE N-BUTYLISOCYANIDE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE METHYL ISOCYANOACETATE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Ethyl isocyanoacetate COBALT(II) ACETYLACETONATE Benzyl isocyanide TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) TERT-BUTYL ISOCYANIDE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Aluminum acetylacetonate Tosylmethyl isocyanide Cupric acetylacetonate Ferric acetylacetonate

  • 4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy)
  • 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
  • DAF-FM DA solution
  • DAF-FM DA (cell permeable)
  • -[9H]xanthen]-3-one
  • Diaminofluorescein-<WBR>FM diacetate
  • -difluoro-spiro[isobenzofuran-1(3H)
  • DAF-FM DA
  • DAF-FM DA - CAS 254109-22-3 - Calbiochem
  • 4-Amino-2',7'-Difluoro-5-(Methylamino)-3-Oxo-3H-Spiro[2-Benzofuran-1,9'-Xanthene]-3',6'-Diyl Diacetate
  • DAF-FM DA [DAF-FM Diacetate]
  • 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein diacetate
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-4-amino-2',7'-difluoro-5-(methylamino)-
  • DAF-FM Diacetate NO fluorescent probe
  • 6-acetyloxy-4-amino-2,7-difluoro-5-(methylamino)-3-oxospiro2-benzofuran-1,9-xanthene-3-yl acetate
  • 254109-22-3