DAF-FM DA
- Product Name
- DAF-FM DA
- CAS No.
- 254109-22-3
- Chemical Name
- DAF-FM DA
- Synonyms
- DAF-FM DA;DAF-FM DA solution;-[9H]xanthen]-3-one;DAF-FM DA (cell permeable);DAF-FM DA [DAF-FM Diacetate];-difluoro-spiro[isobenzofuran-1(3H);Diaminofluorescein-FM diacetate;DAF-FM Diacetate NO fluorescent probe;DAF-FM DA - CAS 254109-22-3 - Calbiochem;4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy)
- CBNumber
- CB21074275
- Molecular Formula
- C25H18F2N2O7
- Formula Weight
- 496.42
- MOL File
- 254109-22-3.mol
DAF-FM DA Property
- Boiling point:
- 695.7±55.0 °C(Predicted)
- Density
- 1.56±0.1 g/cm3(Predicted)
- storage temp.
- -20°C
- solubility
- Soluble in dimethyl sulfoxide
- pka
- 4.20 ± 0.20, most basic, temperature: 25 °C
- form
- Pale yellow solution
- color
- clear clear, colorless
- λmax
- 365 nm (MeOH)
- Biological Applications
- Detecting bacteria/microorganisms; nitric oxide indicator,
Safety
- WGK Germany
- 3
N-Bromosuccinimide Price
- Product number
- D1946
- Product name
- DAF-FM DA
- Purity
- ≥98% (HPLC)
- Packaging
- 1mg
- Price
- $737
- Updated
- 2024/03/01
- Product number
- 251520-M
- Product name
- DAF-FM DA - CAS 254109-22-3 - Calbiochem
- Purity
- A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
- Packaging
- 251520-1MG
- Price
- $1010
- Updated
- 2024/03/01
- Product number
- 251520-M
- Product name
- DAF-FM DA - CAS 254109-22-3 - Calbiochem
- Purity
- A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
- Packaging
- 1mg
- Price
- $957
- Updated
- 2023/01/07
- Product number
- 18767
- Product name
- DAF-FM diacetate
- Purity
- ≥98%
- Packaging
- 1mg
- Price
- $413
- Updated
- 2024/03/01
- Product number
- 18767
- Product name
- DAF-FM diacetate
- Packaging
- 5mg
- Price
- $1543
- Updated
- 2024/03/01
DAF-FM DA Chemical Properties,Usage,Production
Uses
DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495
Uses
DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).
Biochem/physiol Actions
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.
storage
Store at -20°C