ChemicalBook > CAS DataBase List > Collagenase

Collagenase

Description References

Product Name: Collagenase
CAS No.: 9001-12-1
Chemical Name: Collagenase
Synonyms: FIBROBLAST;Collagenase IV;Collagenase clostridium histolyticum;Santyl;INTERSTITIAL;Collagenase A;Collagenase II;COLLAGENASE TYPE XI;COLLAGENASE TYPE VII HIGH PURITY;CLG
CBNumber: CB2112176
Molecular Formula: C60H100N2
Formula Weight: 849.4494
MOL File: 9001-12-1.mol

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Collagenase Property

storage temp.: -20°C
solubility: Soluble in aqueous buffers.
form: powder
color: light brown
PH: 7.0
biological source: Clostridium histolyticum
Water Solubility: water: soluble
Merck: 2481
Specific Activity: 0.5-5.0FALGPA units/mg solid
InChIKey: YRQNKMKHABXEJZ-UVQQGXFZSA-N
CAS DataBase Reference: 9001-12-1
EPA Substance Registry System: Collagenase (9001-12-1)

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Safety

Hazard Codes: Xn
Risk Statements: 36/37/38-42
Safety Statements: 22-24-26-36/37
WGK Germany: 1
F: 10-21
HS Code: 3507907000

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: COLLA-RO
Product name: Collagenase A
Purity: from Clostridium histolyticum
Packaging: 100mg
Price: $88.5
Updated: 2025/07/31

Sigma-Aldrich

Product number: COLLA-RO
Product name: Collagenase A
Purity: from Clostridium histolyticum
Packaging: 500mg
Price: $330
Updated: 2025/07/31

Sigma-Aldrich

Product number: COLLA-RO
Product name: Collagenase A
Purity: from Clostridium histolyticum
Packaging: 2.5g
Price: $1040
Updated: 2025/07/31

Sigma-Aldrich

Product number: C8176
Product name: Collagenase from Clostridium histolyticum
Purity: Sigma Blend Type L, ≤1.0?FALGPA units/mg solid
Packaging: 5G
Price: $5850
Updated: 2025/07/31

Sigma-Aldrich

Product number: C2799
Product name: Collagenase from Clostridium histolyticum
Purity: powder, suitable for cell culture, ≥4?FALGPA units/mg solid, high purity, ≥700?CDU/mg solid (CDU = collagen digestion units)
Packaging: 7500units
Price: $374.85
Updated: 2025/07/31

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Collagenase Chemical Properties,Usage,Production

Description

Collagenases are a category of enzymes that can digest native collagen, the major fibrous component of animal extracellular connective tissue. Collagenases can be produced by some bacterias such as Clostridium and can also be made by the animal body as part of its normal immune response. They normally target the connective tissue in muscle cells and other body organs. They can break peptide bonds within collagen and help create collagen by cleaving pro-collagen, collagen's precursor, once it is secreted by the cell. Bacterial collagenases exhibit broader substrate specificity than animal collagenases and unlike animal collagenases that split collagen in its native triple-helical conformation, bacterial collagenase can degrade both water-insoluble native collagens and water-soluble denatured ones. In addition, bacterial collagenase can attack almost all collagen types, and is able to make multiple cleavages within triple helical regions. Collagenases have a molecular weight ranging from 68 kilodaltons (kDa) to 130 Kda due to differing sizes based on the class to which the isolate belongs. The optimal pH for stability ranges from approximately 6.3 to 7.5 with a theoretical isoelectric point of 5.62. Numerous compounds and molecules have the capability of acting as collagenases activator or inhibitor. For example, Ca2+ and Zn2+ ions are two known activators. On the other hand, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), cysteine, histidine, dithiothreitol (DTT), 2-mercaptoethanol, o-phenanthroline, Hg2+, Pb2+, Cd2+, Cu2+, and Zn2+ are all known inhibitors of collagenase activity.

References

1.https://en.wikipedia.org/wiki/Collagenase
2.http://www.wisegeek.com/what-is-collagenase.htm
3.http://www.rockland-inc.com/collagenase.aspx
4.http://www.worthington-biochem.com/cls/default.html

Uses

This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

Uses

In investigation of the structure and biosynthesis of collagen; in dispersion of cells for tissue culture studies.

Uses

Collagenase, Type I, Clostridium histolyticum is used as a powder and solvent injection kit for the treatment of dupuytren's contracture.

Definition

A rare proteinase that catalyzes the hydrolysis of collagen. Collagenase activity has been demonstrated in several venoms of crotalid and viperid snakes and it occurs in certain species of clostridium.

General Description

Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.

Biochem/physiol Actions

Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.

Purification Methods

Collagenase is purified by using N-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986].

Collagenase Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Collagenase manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Collagenase 9001-12-1
Price: US $0.00-0.00/g
Min. Order: 1g
Purity: 98.0%
Supply Ability: 1kg/month
Release date: 2021-08-27

Hebei Chuanghai Biotechnology Co., Ltd

Product: Collagenase 9001-12-1
Price: US $19.00/ASSAYS
Min. Order: 100ASSAYS
Purity: 99%
Supply Ability: 100 mt
Release date: 2024-11-20

Hebei Chuanghai Biotechnology Co., Ltd

Product: Collagenase 9001-12-1
Price: US $0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 500000kg
Release date: 2024-10-31

9001-12-1, CollagenaseRelated Search:

Papain PROTEASE Pepsin Proteinase K PROTEASE Tribenuron methyl EC 3.4.21.19 Basic protease Chymotrypsin Bovine bone collagen peptide Trypsin METHYL ISOCYANOACETATE Ethyl isocyanoacetate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) PHENYLSELENOL Benzyl isocyanide SALCOMINE FishCollagen

COLLAGENASE TYPE X

Collagenase from Clostridium histolyticum

collagenase crude type ia cell culture*tested

collagenase F. clostridium histolyticum

collagenase from achromobacter iophagus

collagenase sterile filtered type vii-S

collagenase type I-S

Collagenase A

Matrix metalloprotease-1

Metallocollagenase

Santyl

Soycollagestin

(VIAL) COLLAGENASE NB1 GMP GRADE

COLLAGENASE NB4 FROM CL. HIST.STANDARD GRADE

COLLAGENASE NB1 FROM CL. HIST.PREMIUM GRADE

COLLAGENASE NB 8 FROM CL. HISTOLYTICUMBROAD RANGE

COLLAGENASE NB6 FROM CL. HISTOLYTICUMGMP GRADE

COLLAGENASE NB 5 FROM CL. HISTOLYTICUMSTERILE GRADE

COLLAGENASE N REFERENCE CONTENT

COLLAGENASE N REFERENCEIDENTITY

COLLAGENASE NB 4G PROVED GRADE

Collagenase from Clostridium histolyticum,Clostridiopeptidase A

Collagenase,Type-III,from Clostridium histolyticum

Collagenase I

Collagenase II

Collagenase IV

Collagenase V

Collagenase from Clostridium histolyticum,lyophil.

Recombinant Collagenase G >95%

Recombinant Collagenase H >95%

Recombinant Collagenase H/G >70%

Recombinant Collagenase H/G >95%

Collagenase NB 4G Proved Grade froM ClostridiuM histolyticuM crude

Collagenase froM ClostridiuM histolyticuM(Purified)

Anti-MMP1 (Cleaved-Phe100) antibody produced in rabbit

collagenase type vii

Collagenase, Paralithodes camtschatica Hepatopancreas

Collagenase, Type II, Clostridium histolyticum

Collagen-Peptide

COLLAGENASE TYPE IA-S CELL CULTURE TESTED

COLLAGENASE SIGMA BLEND TYPE N

COLLAGENASE SIGMA BLEND TYPE L

COLLAGENASE TYPE XI-S CELL CULTURE TESTED

COLLAGENASE TYPE VII-S CELLCULTURE TESTE D

COLLAGENASE TYPE III FRACTION A

COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE IV

COLLAGENASE F. CLOSTRIDIUM HISTOLYTICUM POWD.0.15-0.4 U/MG*

COLLAGENASE FROM CLOSTRIDIUM HISTOLY-TIC UM, ~0.5-2 U/MG

COLLAGENASE HIGH PURITY TYPE VII CELLCUL TURE TESTE

COLLAGENASE SIGMA BLEND TYPE F

COLLAGENASE SIGMA BLEND TYPE H

COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE VIII

COLLAGENASE TYPE IV-S

COLLAGENASE FROM CLOSTRIDIUM HISTOLY-TIC UM, ~0.1 U/MG

COLLAGENASE STERILE FILTERED TYPE XI-S

COLLAGENACOLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE IA, FOR GENERAL USE

COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE II

COLLAGENASE TYPE XI