ChemicalBook > CAS DataBase List > PNGASE F

PNGASE F

Product Name
PNGASE F
CAS No.
83534-39-8
Chemical Name
PNGASE F
Synonyms
PNGASE A;PNGASE F;EC 3.5.1.52;N-GLYCANASE;GLYCOPEPTIDASE;N-GLYCANASE(R);PNGase F（Fast）;N-GLYCOSIDASE A;N-GLYCOSIDASE F;GLYCOPEPTIDASE A
CBNumber
CB2114680
Formula Weight
0
MOL File
Mol file
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PNGASE F Property

storage temp. 
2-8°C
form 
buffered aqueous solution
color 
Colorless to light yellow
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Safety

Hazard Codes 
Xi,N,Xn
Risk Statements 
37/38-41-50/53-43-20/21/22
Safety Statements 
22-24/25-36/37/39-26-61
RIDADR 
UN 3316 9
WGK Germany 
2
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
P7367
Product name
PNGase F from Elizabethkingia meningoseptica
Purity
BioReagent, ≥95% (SDS-PAGE), for proteomics
Packaging
50units
Price
$407
Updated
2024/03/01
Sigma-Aldrich
Product number
P7367
Product name
PNGase F from Elizabethkingia meningoseptica
Purity
BioReagent, ≥95% (SDS-PAGE), for proteomics
Packaging
300Units
Price
$1800
Updated
2024/03/01
Sigma-Aldrich
Product number
G5166
Product name
PNGase F from Elizabethkingia miricola
Purity
buffered aqueous solution
Packaging
50units
Price
$330
Updated
2024/03/01
Sigma-Aldrich
Product number
G5166
Product name
PNGase F from Elizabethkingia miricola
Purity
buffered aqueous solution
Packaging
100units
Price
$540
Updated
2024/03/01
Sigma-Aldrich
Product number
G1549
Product name
PNGase F from Elizabethkingia meningoseptica
Purity
buffered aqueous solution, recombinant, expressed in E. coli
Packaging
50units
Price
$272
Updated
2024/03/01
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PNGASE F Chemical Properties,Usage,Production

Uses

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Uses

PNGase F from Elizabethkingia meningoseptica has been used:

  • for de-N-glycosylation of Zika20virus E protein
  • to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
  • to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Uses

Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.

General Description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGASE F Preparation Products And Raw materials

Raw materials

Preparation Products

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PNGASE F Suppliers

China 48 Japan 3 Switzerland 1 Ukraine 1 United Kingdom 1 United States 8 Global 62
Nacalai Tesque, Inc.
Tel
--
Fax
--
Email
info-tech@nacalai.co.jp
Country
Japan
ProdList
6046
Advantage
75
Wako Pure Chemical Industries, Ltd.
Tel
--
Fax
--
Email
labchem-tec@wako-chem.co.jp
Country
Japan
ProdList
6819
Advantage
80
Kanto Chemical Co., Inc.
Tel
--
Fax
--
Email
reag-info@gms.kanto.co.jp
Country
Japan
ProdList
6756
Advantage
74

83534-39-8, PNGASE FRelated Search:

METHYL ISOCYANOACETATE Ethyl isocyanoacetate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Benzyl isocyanide TERT-BUTYL ISOCYANIDE Cupric acetylacetonate 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Aluminum acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III) Ferric acetylacetonate PHENYLSELENOL TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Tosylmethyl isocyanide SALCOMINE N-BUTYLISOCYANIDE Gentamicin sulfate

  • N-Glycosidase A, PNGase A, N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase
  • N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum
  • PNGaseF from Elizabethkingia meningoseptica
  • PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, RECOMBINANT EXPRESSED IN ESCHERICHIA CO
  • PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, PROTEOMICS GRADE
  • peptide-N4-(N-acetyl--glucosaMinyl) asparagineaMidase
  • Glycopeptidase F from Elizabethkingia meningoseptica
  • PNGase F from Elizabethkingia miricola
  • N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase
  • EC 3.5.1.52
  • GLYCOPEPTIDASE
  • GLYCOPEPTIDASE A
  • GLYCOPEPTIDASE F
  • N-LINKED-GLYCOPEPTIDE-[N-ACETYL-BETA-D-GLUCOSAMINYL]-L-ASPARAGINE AMIDOHYDROLASE
  • N-GLYCANASE
  • N-GLYCANASE(R)
  • N-GLYCOSIDASE A
  • N-GLYCOSIDASE A, ALMOND
  • N-GLYCOSIDASE F
  • N-GLYCOSIDASE F, CHRYSEOBACTERIUM MENINGOSEPTICUM
  • PNGASE A
  • PNGASE F
  • PNGASE (F CHRYSEOBACTERIUM (FLAVOBACTERIUM) MENINGOSEPTICUM)
  • PEPTIDE-N4-(ACETYL-BETA-GLUCOSAMINYL)-ASPARAGINE AMIDASE
  • (PEPTIDE-N4-[N-ACETYL-BETA-GLUCOSAMINYL)]
  • PEPTIDE N-GLYCOSIDASE
  • PEPTIDE-N-GLYCOSIDASE F
  • PNGASE F HIGH CONCENTRATION FROM CHRYSEO
  • PNGASE F RECOMBINANT CHRYSEOBACTERIUM
  • GLYCOPEPTIDASE FROM ALMONDS
  • glycopeptidase a from almonds
  • pngase f from elizabethkingia (chryseobacterium/flavobacterium) meningsepticum
  • GLYCOPEPTIDASE F, RECOMBINANT, SOLUTION, FROM FLAVOBACTERIUM MENINGOSEPTICUM
  • N-Glycanase, N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum
  • N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli
  • N-Glycosidase F, Elizabethkingia meningosepticum
  • Native Almonds Glycopeptidase A
  • Native Elizabethkingia meningoseptica PNGase F
  • PNGase F from Elizabethkingia miricola, Recombinant
  • PNGase F from Flavobacterium meningosepticum, Recombinant
  • Native Elizabethkingia miricola PNGase F
  • Native Flavobacterium meningosepticum PNGase F
  • PNGase F from Elizabethkingia meningoseptica, Recombinant
  • peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase
  • buffered aqueous solution, recombinant, expressed in E. coli
  • PNGase F from Elizabethkingia meningoseptica lyophilized powder, recombinant, expressed in E. coli
  • Glycopeptidase A from almonds buffered aqueous glycerol solution, >=0.05 unit/mL
  • PNGase F from Elizabethkingia miricola buffered aqueous solution
  • PNGase F from Elizabethkingia meningoseptica BioReagent, >=95% (SDS-PAGE), for proteomics
  • PNGase F from Elizabethkingia meningoseptica buffered aqueous solution, recombinant, expressed in E. coli
  • PNGase F（Fast）
  • 83534-39-8
  • Post-translational Modification
  • Proteomics and Protein Expression
  • Proteomics
  • Enzymes for Protein Carbohydrate Modification Analysis
  • Endoglycosidases
  • Enzymes, Inhibitors, and Substrates