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5,5′-Dithiobis(2-nitrobenzoic acid)

Product Name
5,5′-Dithiobis(2-nitrobenzoic acid)
CAS No.
69-78-3
Chemical Name
5,5′-Dithiobis(2-nitrobenzoic acid)
Synonyms
DTNB;Ba-2767;5,5'Ellmanns;5,5&priMe;Ellmsn'sreagent;ELLMANS' REAGENT;ELLMAN'S REAGENT;ellmann's reagent;SAPONIN BIOCHEMICAL
CBNumber
CB2215444
Molecular Formula
C14H8N2O8S2
Formula Weight
396.35
MOL File
69-78-3.mol
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5,5′-Dithiobis(2-nitrobenzoic acid) Property

Melting point:
240-245 °C (dec.)(lit.)
Boiling point:
671.9±55.0 °C(Predicted)
Density 
1.67
refractive index 
1.6900 (estimate)
storage temp. 
Keep in dark place,Sealed in dry,Room Temperature
form 
Yellow solid
pka
1.57±0.25(Predicted)
color 
Pale Yellow to Light Yellow
Water Solubility 
Soluble in water and ethanol.
Merck 
14,3551
BRN 
1896821
Stability:
Stable. Incompatible with strong bases, strong oxidizing agents.
InChIKey
KIUMMUBSPKGMOY-UHFFFAOYSA-N
CAS DataBase Reference
69-78-3(CAS DataBase Reference)
EPA Substance Registry System
Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
3
RTECS 
DG9650000
F 
10
TSCA 
Yes
HS Code 
29309070
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
D218200
Product name
5,5′-Dithiobis(2-nitrobenzoic acid)
Purity
ReagentPlus , 99%
Packaging
1g
Price
$32.3
Updated
2024/03/01
Sigma-Aldrich
Product number
322123
Product name
5,5ʹ-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem
Purity
Sulfhydryl reagent used to characterize reactive SH groups.
Packaging
5G
Price
$140
Updated
2024/03/01
TCI Chemical
Product number
D0944
Product name
5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]
Purity
>98.0%(HPLC)(T)
Packaging
1g
Price
$29
Updated
2024/03/01
TCI Chemical
Product number
D0944
Product name
5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]
Purity
>98.0%(HPLC)(T)
Packaging
5g
Price
$75
Updated
2024/03/01
Alfa Aesar
Product number
A14331
Product name
5,5'-Dithiobis(2-nitrobenzoic acid), 99%
Packaging
1g
Price
$34.9
Updated
2024/03/01
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5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties,Usage,Production

Chemical Properties

Crystalline

Uses

Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

Uses

5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.

Uses

5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.

Definition

ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.

General Description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

5,5′-Dithiobis(2-nitrobenzoic acid) Preparation Products And Raw materials

Raw materials

Preparation Products

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5,5′-Dithiobis(2-nitrobenzoic acid) Suppliers

Americas 1 Austria 1 Canada 1 China 270 Europe 4 France 1 Germany 9 India 15 Japan 5 South Korea 1 Switzerland 6 United Kingdom 16 United States 46 Global 376
TOKYO CHEMICAL INDUSTRY CO., LTD.
Tel
03-36680489
Fax
03-3668-0520
Email
Sales-JP@TCIchemicals.com
Country
Japan
ProdList
28398
Advantage
80
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View Lastest Price from 5,5′-Dithiobis(2-nitrobenzoic acid) manufacturers

Henan Fengda Chemical Co., Ltd
Product
5,5′-Dithiobis(2-nitrobenzoic acid) 69-78-3
Price
US $100.00-1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2023-12-24
Hebei Yanxi Chemical Co., Ltd.
Product
5,5′-Dithiobis(2-nitrobenzoic acid) 69-78-3
Price
US $100.00-10.00/kgkg
Min. Order
1kgkg
Purity
0.99
Supply Ability
10000
Release date
2023-09-28
WUHAN FORTUNA CHEMICAL CO., LTD
Product
5,5′-Dithiobis(2-nitrobenzoic acid)/DTNB 69-78-3
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%min
Supply Ability
100KG
Release date
2021-06-01

69-78-3, 5,5′-Dithiobis(2-nitrobenzoic acid)Related Search:

Thiamine disulfide LITHIUM PERIODATE Folic acid Ethyl 2-(Chlorosulfonyl)acetate 4-Nitrobenzoic acid 2-Nitrobenzoic acid 4-Nitrophenyl chloroformate Ascoric Acid 3,3'-DICARBOXYLIC ACID DIPHENYL DISULFIDE 4,4'-Dithiodianiline METHYL PHENYL DISULFIDE 4,4'-Dinitrodiphenyl disulfide 2-Nitrobenzaldehyde DIPHENYL DISULFIDE 4-NITROTHIOPHENOL 2-NITROSOTOLUENE 2-Nitrobenzyl alcohol Thiobenzoic acid

  • DTNB
  • ellmann's reagent
  • ELLMAN'S REAGENT
  • ELLMANS' REAGENT
  • BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE
  • 3-CARBOXY-4-NITROPHENYL DISULFIDE
  • Ellmsn'sreagent
  • Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans
  • Ellmanns
  • 5,5'-Dithobis(2-Nitrobenzoic Acid)
  • 5,5'-DITHIO-BIS(2-NITROBENZOIC ACID) (3, 3'-6)
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  • Dithiobis(2-nitrobenzoic acid)
  • 5,5-Dithiobis(2-nitrobenzoic acid)product (DTNB)
  • Benzoic acid, 3,3-dithiobis6-nitro-
  • 5-[(3-CARBOXY-4-NITROPHENYL)DITHIO]-2-NITROBENZOICACID(5,5-DITHIOBIS(2-NITROBENZOICACID))
  • 5,5-Dithiobis(2-nitrobenzoic acid) (DTNB)
  • 5,5'-DITHIOBIS(2-NITROBENZOIC ACID)
  • 5,5-DITHIO-BIS(2-NITROBENZOIC ACID)
  • 5-[(3-CARBOXY-4-NITROPHENYL)DITHIO]-2-NITROBENZOIC ACID
  • 6,6'-DINITRO-3,3'-DITHIODIBENZOIC ACID
  • 2,2'-DINITRO-5,5'-DITHIODIBENZOIC ACID
  • dithionitrobenzoicacid
  • (3-Carboxy-4-nitrophenyl)disulphide, DTNB, Ellman's reagent
  • 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]
  • 5,5-DITHIOBIS(2-NITROBENZOIC ACID) extrapure
  • 5,5'-Dithiobis(2-nitrobenzoic acid) Vetec(TM) reagent grade, 98%
  • SAPONIN BIOCHEMICAL
  • DTNB, 5,5'-Dithiobis-(2-nitrobenzoic acid)
  • DTNB Ellman's Reagent
  • 2,2’-dinitro-5,5’-dithiodibenzoesaeure
  • 3,3’-dithiobis(6-nitro-benzoicaci
  • 3,3’-dithiobis(6-nitrobenzoicacid)
  • 3,3’-dithiobis[6-nitro-benzoicaci
  • 5,5’-dithiobis(2-nitrobenzoic
  • 3-Carboxy-4-nitrophenyl disulfide, 6,6μ-Dinitro-3,3μ-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellmans Reagent
  • 5,5'-Dithiobis(2-nitrobenzoic acid) ,98%
  • 5,5'-DITHIOBIS(2-NITROBENZOICACID) RESEARCH GRADE
  • 5,5′-Dithiobis(2-nitrobenzoic acid),3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent
  • 3-Carboxy-4-nitrophe
  • 5-(3-Carboxy-4-nitrophenyl)disulfanyl-2-nitrobenzoic acid
  • 5,5'-Dithiobis(2-nitrobenzoic acid) ,99%
  • Ba-2767
  • Di(3-carboxy-4-nitrophenyl) persulfide
  • Di(3-carboxy-4-nitrophenyl)persulfide
  • Bis(3-carboxy-4-nitrophenyl) Disulphide
  • Bis(3-carboxy-4-nitrophenyl) Disulfide DTNB Ellman's Reagent
  • 5-Dithiobis(2-nitrobenzoic acid)
  • 5,5'-disulfanediylbis(2-nitrobenzoic acid)
  • 5,5&priMe
  • 5,5 'twothiobis(2-Nitrobenzoic Acid
  • 5,5'-Dithiobis(2-nitrobenzoic acid) ReagentPlus(R), 99%
  • Dithiobis(2-nitrobenzoic acid) research grade
  • 5,5-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem
  • 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]&gt
  • ithionitrobenzoicaci
  • 5,5′-Dithiobis(2-nitrobenzoic acid) ISO 9001：2015 REACH
  • 3-Carboxy-4-nitrophenyl disulfide 69-78-3