ChemicalBook > CAS DataBase List > GLUCOSE-6-PHOSPHATE DEHYDROGENASE

GLUCOSE-6-PHOSPHATE DEHYDROGENASE

Product Name
GLUCOSE-6-PHOSPHATE DEHYDROGENASE
CAS No.
9001-40-5
Chemical Name
GLUCOSE-6-PHOSPHATE DEHYDROGENASE
Synonyms
G-6-PD;C:C&B31;G-6-P-DH;EC 1.1.1.49;EC: 1.1.1.49;IUB: 1.1.1.49;G-6-PDH TYPE V;ZWISCHENFERMENT;G-6-PDH TYPE IX;G-6-PDH TYPE XV
CBNumber
CB3211458
Molecular Formula
n.a.
Formula Weight
0
MOL File
Mol file
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GLUCOSE-6-PHOSPHATE DEHYDROGENASE Property

Melting point:
~120℃
storage temp. 
-20°C
form 
suspension
color 
white
Water Solubility 
Soluble in water and dilute buffer.
EPA Substance Registry System
Dehydrogenase, glucose 6-phosphate (9001-40-5)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-24/25
WGK Germany 
3
3-10
TSCA 
Yes
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
G8529
Product name
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity
recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging
200units
Price
$67.3
Updated
2024/03/01
Sigma-Aldrich
Product number
G8529
Product name
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity
recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging
2000units
Price
$156.6
Updated
2024/03/01
Sigma-Aldrich
Product number
G8529
Product name
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity
recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging
1000units
Price
$174
Updated
2024/03/01
Sigma-Aldrich
Product number
G8529
Product name
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity
recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging
10000units
Price
$854
Updated
2024/03/01
Sigma-Aldrich
Product number
G8404
Product name
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity
recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550?units/mg protein (biuret)
Packaging
200units
Price
$68.8
Updated
2024/03/01
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GLUCOSE-6-PHOSPHATE DEHYDROGENASE Chemical Properties,Usage,Production

Chemical Properties

White to yellow powder

Uses

Glucose-6-phosphate dehydrogenase, Leuconostoc mesenteroides is used to convert p-glucose-6-phosphate to p-glucono-d-lactone-6-phosphate in presence of NADP+ or NAD+. It is used as an intermediate, chemical and research purpose and also used fo analysis.

Uses

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

Uses

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

General Description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.

Purification Methods

The enzyme is useful for measuring pyridine nucleotides in enzyme recycling. The enzyme from Baker's yeast has been purified by (NH4)2SO4 fractionation, Me2CO precipitation, a second (NH4)2SO4 fractionation, concentration by DEAE-SF chromatography, a third (NH4)2SO4 fractionation and recrystallisation. Crystallisation is induced by addition of its coenzyme NADP, which in its presence causes rapid separation of crystals at (NH4)2SO4 concentration much below that required to precipitate the amorphous enzyme. To recrystallise, the crystals are dissolved in 0.01M NADP (pH 7.3) with (NH4)2SO4 at 0.55 saturation, and the crystals appear within 10 to 60minutes. After standing for 2-3days (at 4o) the (NH4)2SO4 is increased to 0.60 of saturation, and more than 80% of the activity in the original crystals is recovered in the fresh crystals. [Noltmann et al. J Biol Chem 236 1255 1961]. Large amounts can be obtained from rat livers. The livers are extracted with 0.025M phosphate buffer (pH 7.5) and precipitated with 3M (NH4)2SO4 (70% of activity). The precipitate is dissolved in 3volumes of 0.025M phosphate (pH 7.5), dialysed against this buffer + 0.2mM EDTA at 4o for 5hours, then diluted to 1% protein and the nucleic acids are precipitated by addition of 0.4volumes of 1% protamine sulfate. (NH4)2SO4 is added to a concentration of 2M (pH adjusted to 7.0 with NH3), the precipitate is discarded and the supernatant is adjusted to 2.8M (NH4)2SO4, dialysed, and the protein is adjusted to 1% and treated with Ca3(PO4)2 gel. The gel is added in three steps (1.5mL of 0.4% gel/mL per step), and the gel is removed by centrifugation after each addition. The third gel adsorbed 50% of the activity. The gel is eluted with 0.2M phosphate buffer (pH 7.4, 40mL/g of gel; 60% recovery). The extract is precipitated in 3volumes of (NH4)2SO4 (adjusted to 4M) to give enzyme with an activity of 30Wmoles/mg of protein per hour. [Lowry et al. J Biol Chem 236 2746 1961.] The Km values for the yeast enzyme are 20WM for G-6P and 2WM for NADP (Tris pH 8.0, 10-2 M MgCl2, 38o) [Noltmann & Kuby The Enzymes VII 223 1963].

GLUCOSE-6-PHOSPHATE DEHYDROGENASE Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from GLUCOSE-6-PHOSPHATE DEHYDROGENASE manufacturers

Career Henan Chemical Co
Product
GLUCOSE-6-PHOSPHATE DEHYDROGENASE 9001-40-5
Price
US $1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
25kg
Release date
2019-07-11

9001-40-5, GLUCOSE-6-PHOSPHATE DEHYDROGENASERelated Search:


  • glucose-6-phosphate dehydrogenase from baker's yeast (s. cerevisiae)
  • Glucose -6-phosphoric acid dehydrogenase
  • ZWISCHENFERMENT
  • ZWISCHENFERMENT G6P-DH
  • ZWISCHENFERMENT TYPE IX
  • ZWISCHENFERMENT TYPE V
  • ZWISCHENFERMENT TYPE VII
  • ZWISCHENFERMENT TYPE XV
  • ZWISCHENFERMENT TYPE XV-B
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE, LEUCONOSTOC MESENTEROIDES
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE IX
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE V
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE VII
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV-B
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIII
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIV
  • G-6-P-DH
  • G-6-PDH-BIOTIN LABELED
  • G-6-PDH TYPE IX
  • G-6-PDH TYPE V
  • G-6-PDH TYPE VII
  • G-6-PDH TYPE XV
  • G-6-PDH TYPE XV-B
  • IUB: 1.1.1.49
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE X IX
  • G-3-Pdh(Glycerol-3-PhosphateDehydrogenase),Mesenteroides
  • fromyeastsaltfreepowder400unitsmg
  • Glucose-6-phosphate dehydrogenase from yeast ca. 140 U/mg protein
  • Glucose-6-phosphate.dehydrogenase from Leuconostoc mesenteroides
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE extrapure
  • Glucose-6-phosphate Dehydrogenase Biotin Labeled from baker's yeast
  • Glucose-6-phosphate Dehydrogenase from yeast
  • G-6-P-DH, Zwischenferment
  • Glucose-6-phosphate Dehydrogenase-Agarose from baker's yeast (S.cerevisiae)
  • glicose-6-phosphate dehydrogenase, yeast
  • Glucose-6-Phosphate Dehydrogenase, Lyophilized
  • Glucose-6-Phosphate Dehydrogenase,from Yeast, 500U/mg protein
  • Glucose-6-phosphate dehydrogenase [400 units/mg]
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE, TORU- LA YEAST, ~180 U/MG
  • Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae),G-6-P-DH, Zwischenferment
  • Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides,G-6-P-DH
  • Glucose-6-phosphate dehydrogenase [>= 150 units/mg solid]
  • G6P-DH(Glucose-6-Phosphate Dehydrogenase)
  • Glucose-6-Phosphate Dehydrogenase,G6PD
  • 6-Phosphoglucose dehydrogenase
  • D-GLUCOSE-6-PHOSPHATE: NAD(P)+ 1-OXIDOREDUCTASE
  • D-GLUCOSE-6-PHOSPHATE: NADP 1-OXIDOREDUCTASE
  • EC 1.1.1.49
  • EC: 1.1.1.49
  • Dehydrogenase,glucose6-phosphate
  • glucose-6-phosphatedehydrogenasefromyeastsusp~120u/mg
  • G-6-PDH, D-Glucose-6-phosphate: NADP 1-oxidoreductase
  • Glucose-6-phosphate Dehydrogenase, 500 units/Mg
  • G-6-pdh control
  • Glucose-6-phosphate Dehydrogenase from torula yeast
  • GLUCOSE-6-PHOSPHATE DEHYDROGENASE FROM &