GLUCOSE-6-PHOSPHATE DEHYDROGENASE

ChemicalBook > CAS DataBase List > GLUCOSE-6-PHOSPHATE DEHYDROGENASE

GLUCOSE-6-PHOSPHATE DEHYDROGENASE

Product Name: GLUCOSE-6-PHOSPHATE DEHYDROGENASE
CAS No.: 9001-40-5
Chemical Name: GLUCOSE-6-PHOSPHATE DEHYDROGENASE
Synonyms: G-6-PD;C:C&B31;G-6-P-DH;EC 1.1.1.49;EC: 1.1.1.49;IUB: 1.1.1.49;G-6-PDH TYPE V;ZWISCHENFERMENT;G-6-PDH TYPE IX;G-6-PDH TYPE XV
CBNumber: CB3211458
Molecular Formula: n.a.
Formula Weight: 0
MOL File: Mol file

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GLUCOSE-6-PHOSPHATE DEHYDROGENASE Property

Melting point:: ～120℃
storage temp.: -20°C
form: suspension
color: white
Water Solubility: Soluble in water and dilute buffer.
EPA Substance Registry System: Dehydrogenase, glucose 6-phosphate (9001-40-5)

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 26-36-24/25
WGK Germany: 3
F: 3-10
TSCA: Yes
HS Code: 35079090

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: G8529
Product name: Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity: recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging: 200units
Price: $67.3
Updated: 2024/03/01

Sigma-Aldrich

Product number: G8529
Product name: Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity: recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging: 2000units
Price: $156.6
Updated: 2024/03/01

Sigma-Aldrich

Product number: G8529
Product name: Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity: recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging: 1000units
Price: $174
Updated: 2024/03/01

Sigma-Aldrich

Product number: G8529
Product name: Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity: recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret)
Packaging: 10000units
Price: $854
Updated: 2024/03/01

Sigma-Aldrich

Product number: G8404
Product name: Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Purity: recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550?units/mg protein (biuret)
Packaging: 200units
Price: $68.8
Updated: 2024/03/01

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GLUCOSE-6-PHOSPHATE DEHYDROGENASE Chemical Properties,Usage,Production

Chemical Properties

White to yellow powder

Uses

Glucose-6-phosphate dehydrogenase, Leuconostoc mesenteroides is used to convert p-glucose-6-phosphate to p-glucono-d-lactone-6-phosphate in presence of NADP+ or NAD+. It is used as an intermediate, chemical and research purpose and also used fo analysis.

Uses

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

Uses

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

General Description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.

Purification Methods

The enzyme is useful for measuring pyridine nucleotides in enzyme recycling. The enzyme from Baker's yeast has been purified by (NH4)2SO4 fractionation, Me2CO precipitation, a second (NH4)2SO4 fractionation, concentration by DEAE-SF chromatography, a third (NH4)2SO4 fractionation and recrystallisation. Crystallisation is induced by addition of its coenzyme NADP, which in its presence causes rapid separation of crystals at (NH4)2SO4 concentration much below that required to precipitate the amorphous enzyme. To recrystallise, the crystals are dissolved in 0.01M NADP (pH 7.3) with (NH4)2SO4 at 0.55 saturation, and the crystals appear within 10 to 60minutes. After standing for 2-3days (at 4o) the (NH4)2SO4 is increased to 0.60 of saturation, and more than 80% of the activity in the original crystals is recovered in the fresh crystals. [Noltmann et al. J Biol Chem 236 1255 1961]. Large amounts can be obtained from rat livers. The livers are extracted with 0.025M phosphate buffer (pH 7.5) and precipitated with 3M (NH4)2SO4 (70% of activity). The precipitate is dissolved in 3volumes of 0.025M phosphate (pH 7.5), dialysed against this buffer + 0.2mM EDTA at 4o for 5hours, then diluted to 1% protein and the nucleic acids are precipitated by addition of 0.4volumes of 1% protamine sulfate. (NH4)2SO4 is added to a concentration of 2M (pH adjusted to 7.0 with NH3), the precipitate is discarded and the supernatant is adjusted to 2.8M (NH4)2SO4, dialysed, and the protein is adjusted to 1% and treated with Ca3(PO4)2 gel. The gel is added in three steps (1.5mL of 0.4% gel/mL per step), and the gel is removed by centrifugation after each addition. The third gel adsorbed 50% of the activity. The gel is eluted with 0.2M phosphate buffer (pH 7.4, 40mL/g of gel; 60% recovery). The extract is precipitated in 3volumes of (NH4)2SO4 (adjusted to 4M) to give enzyme with an activity of 30Wmoles/mg of protein per hour. [Lowry et al. J Biol Chem 236 2746 1961.] The Km values for the yeast enzyme are 20WM for G-6P and 2WM for NADP (Tris pH 8.0, 10-2 M MgCl2, 38o) [Noltmann & Kuby The Enzymes VII 223 1963].

GLUCOSE-6-PHOSPHATE DEHYDROGENASE Preparation Products And Raw materials

Raw materials

Ribonucleic acid CELLULOSE PHOSPHATE

Preparation Products

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View Lastest Price from GLUCOSE-6-PHOSPHATE DEHYDROGENASE manufacturers

Career Henan Chemical Co

Product: GLUCOSE-6-PHOSPHATE DEHYDROGENASE 9001-40-5
Price: US $1.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 25kg
Release date: 2019-07-11

9001-40-5, GLUCOSE-6-PHOSPHATE DEHYDROGENASERelated Search:

TERT-BUTYL ISOCYANIDE Aluminum acetylacetonate COBALT(II) ACETYLACETONATE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE SALCOMINE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) N-BUTYLISOCYANIDE Tris(2,4-pentanedionato)chroMiuM(III) DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) PHENYLSELENOL 2,4-PENTANEDIONE, SILVER DERIVATIVE Tosylmethyl isocyanide Cupric acetylacetonate Benzyl isocyanide METHYL ISOCYANOACETATE Ethyl isocyanoacetate Ferric acetylacetonate

glucose-6-phosphate dehydrogenase from baker's yeast (s. cerevisiae)

Glucose -6-phosphoric acid dehydrogenase

ZWISCHENFERMENT

ZWISCHENFERMENT G6P-DH

ZWISCHENFERMENT TYPE IX

ZWISCHENFERMENT TYPE V

ZWISCHENFERMENT TYPE VII

ZWISCHENFERMENT TYPE XV

ZWISCHENFERMENT TYPE XV-B

GLUCOSE-6-PHOSPHATE DEHYDROGENASE

GLUCOSE-6-PHOSPHATE DEHYDROGENASE, LEUCONOSTOC MESENTEROIDES

GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE IX

GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE V

GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE VII

GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV

GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XV-B

GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIII

GLUCOSE-6-PHOSPHATE DEHYDROGENASE TYPE XXIV

G-6-P-DH

G-6-PDH-BIOTIN LABELED

G-6-PDH TYPE IX

G-6-PDH TYPE V

G-6-PDH TYPE VII

G-6-PDH TYPE XV

G-6-PDH TYPE XV-B

IUB: 1.1.1.49

GLUCOSE-6-PHOSPHATE DEHYDROGENASE*TYPE X IX

G-3-Pdh(Glycerol-3-PhosphateDehydrogenase),Mesenteroides

fromyeastsaltfreepowder400unitsmg

Glucose-6-phosphate dehydrogenase from yeast ca. 140 U/mg protein

Glucose-6-phosphate.dehydrogenase from Leuconostoc mesenteroides

GLUCOSE-6-PHOSPHATE DEHYDROGENASE extrapure

Glucose-6-phosphate Dehydrogenase Biotin Labeled from baker's yeast

Glucose-6-phosphate Dehydrogenase from yeast

G-6-P-DH, Zwischenferment

Glucose-6-phosphate Dehydrogenase-Agarose from baker's yeast (S.cerevisiae)

glicose-6-phosphate dehydrogenase, yeast

Glucose-6-Phosphate Dehydrogenase, Lyophilized

Glucose-6-Phosphate Dehydrogenase,from Yeast, 500U/mg protein

Glucose-6-phosphate dehydrogenase [400 units/mg]

GLUCOSE-6-PHOSPHATE DEHYDROGENASE, TORU- LA YEAST, ~180 U/MG

Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae),G-6-P-DH, Zwischenferment

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides,G-6-P-DH

Glucose-6-phosphate dehydrogenase [>= 150 units/mg solid]

G6P-DH(Glucose-6-Phosphate Dehydrogenase)

Glucose-6-Phosphate Dehydrogenase,G6PD

6-Phosphoglucose dehydrogenase

D-GLUCOSE-6-PHOSPHATE: NAD(P)+ 1-OXIDOREDUCTASE

D-GLUCOSE-6-PHOSPHATE: NADP 1-OXIDOREDUCTASE

EC 1.1.1.49

EC: 1.1.1.49

Dehydrogenase,glucose6-phosphate

glucose-6-phosphatedehydrogenasefromyeastsusp~120u/mg

G-6-PDH, D-Glucose-6-phosphate: NADP 1-oxidoreductase

Glucose-6-phosphate Dehydrogenase, 500 units/Mg

G-6-pdh control

Glucose-6-phosphate Dehydrogenase from torula yeast

GLUCOSE-6-PHOSPHATE DEHYDROGENASE FROM &