ChemicalBook > CAS DataBase List > Aprotinin

Aprotinin

Product Name
Aprotinin
CAS No.
9087-70-1
Chemical Name
Aprotinin
Synonyms
BPTI;Bovine pancreatic trypsin inhibitor;fosten;rp-9921;TRASYLOL;onquinin;repulson;riker52g;trazinin;zymofren
CBNumber
CB3329436
Molecular Formula
C284H432N84O78R2S7
Formula Weight
6495.43988
MOL File
9087-70-1.mol
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Aprotinin Property

Melting point:
>234oC (dec.)
storage temp. 
2-8°C
solubility 
glycerol: soluble3mg/mL, clear, colorless (equilibration buffer containing 5% glycerol)
form 
lyophilized powder
color 
white
Water Solubility 
Freely soluble in water and in aqueous buffers of low ionic strength.
Merck 
13,761
Sequence
H-Pro-Asp-Phe-Cys-Leu-Glu-Pro-Pro-Tyr-Thr-Gly-Pro-Cys-Lys-Ala-Arg-Ile-Ile-Arg-Tyr-Phe-Tyr-Asn-Ala-Lys-Ala-Gly-Leu-Cys-Gln-Thr-Phe-Val-Tyr-Gly-Gly-Cys-Arg-Ala-Lys-Arg-Asn-Asn-Phe-Lys-Ser-Ala-Glu-Asp-Cys-Met-Arg-Thr-Cys-Gly-Gly-Ala-OH
Stability:
Stable. Incompatible with strong oxidizing agents.
InChIKey
RWLBZZCCAZDWSE-BNRYNCRGNA-N
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
42/43-36/37/38-20/21/22
Safety Statements 
22-45-36/37-36-26
WGK Germany 
1
RTECS 
YN5080000
F 
10-21
HS Code 
35040000
Hazardous Substances Data
9087-70-1(Hazardous Substances Data)
Toxicity
LD50 i.v. in mice: 2500000 kallikrein inhibitor units/kg (Trautschold)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
ROAPRO
Product name
Aprotinin
Purity
from bovine lung
Packaging
10mg
Price
$139
Updated
2024/03/01
Sigma-Aldrich
Product number
ROAPRO
Product name
Aprotinin
Purity
from bovine lung
Packaging
50mg
Price
$524
Updated
2024/03/01
Sigma-Aldrich
Product number
ROAPRO
Product name
Aprotinin
Purity
from bovine lung
Packaging
100mg
Price
$775
Updated
2024/03/01
Sigma-Aldrich
Product number
A6103
Product name
Aprotinin bovine
Purity
recombinant,expressedinNicotiana(tobacco),≥5?TIU/mgprotein,≥98%(SDS-PAGE
Packaging
1mg
Price
$166
Updated
2024/03/01
Sigma-Aldrich
Product number
A6103
Product name
Aprotinin bovine
Purity
recombinant,expressedinNicotiana(tobacco),≥5?TIU/mgprotein,≥98%(SDS-PAGE
Packaging
25mg
Price
$469
Updated
2024/03/01
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Aprotinin Chemical Properties,Usage,Production

Description

Aprotinin is a reversible inhibitor of serine proteases such as trypsin (Ki = 0.06 pM), chymotrypsin (Ki = 9.5 nM), and kallikrein (Ki = 0.8 nM). It is a much weaker inhibitor of thrombin (Ki = 0.1-0.8 mM) and trypsinogen (Ki = 2 μM). Aprotinin also competitively inhibits nNOS and iNOS with Ki values of 50 and 78 μM, respectively. Aprotinin is widely used as a protein purification tool to prevent proteases present in tissue samples from degrading the protein of interest.

Chemical Properties

white powder

Originator

Trasylol,Bayer

Uses

A potent protease inhibitor.Aprotinin acts as a reversible serine protease blocking and a binding agent. It is widely employed as an inhibitor of trypsin. It is also used as a proteolytic inhibitor in radioimmunoassays of polypeptide hormones. It is involved in the purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin. Further, it is used in the quantification of kallikrein activity in mixtures of esterases and proteases.

Uses

Aprotinin is largely used as an inhibitor of trypsin.

Manufacturing Process

1 kg of parotid of cattle, which were freed of fat and flesh are comminuted in a meat chopper and twice extracted with 5 L of acetone. The acetone was removed as far as possible by sucking off and the moist material is digested for 2.5 hours in 1.2 L of 1 N acetic acid and 2.8 L of 96% ethanol at 50°C. The filtrate containts about 230.000 inactivator units.
The alcoholic solution is concentrated to 1.2 L by evaporating in vacuo and shaken with an equal amount of ether. Dark coloring matters and residues of fat are thus dissolved. The aqueous phase is now mixed with acetone until a precipitate occurs and the dissolved in dilute acetic acid. The solution is brought to a weakly alkaline pH by addition of ammonia. It is then centrifuged and the inactivator is precipitated with five times the amount of ethanol. The yield amounts to about 180.000 kallikrein inactivator units in 0.4-0.5 g of substance.

brand name

Trasylol (Bayer).

Therapeutic Function

Proteinase inhibitor

General Description

Trypsin inhibitor, pancreas type from bovine lung. It is known as Pancreatic trypsin inhibitor (BPTI). Aprotinin, also known as pancreatic trypsin inhibitor and trypsin-kallikrein inhibitor, is found to be expressed in lungs, spleen, liver, and pancreas. It is also found to be present in the free form in calf serum.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Safety Profile

Experimental reproductive effects. When heated to decomposition it emits toxic fumes of NOx and SOx.

storage

Store at +4°C

References

1. henry da, carless pa, moxey aj, et al. anti-fibrinolytic use for minimising perioperative allogeneic blood transfusion. cochrane database syst rev 2007; 4: cd001886.2. levi m, cromheecke me, de jonge e, et al. pharmacological strategies to decrease excessive blood loss in cardiac surgery: a meta-analysis of clinically relevant endpoints. lancet 1999; 354: 1940-7.3. sedrakyan a, treasure t, elefteriades ja. effect of aprotinin on clinical outcomes in coronary artery bypass graft surgery: a systematic review and meta-analysis of randomized

Aprotinin Preparation Products And Raw materials

Raw materials

Trichloroacetic acid

Preparation Products

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View Lastest Price from Aprotinin manufacturers

Henan Fengda Chemical Co., Ltd
Product
Aprotinin 9087-70-1
Price
US $8.00-0.80/KG
Min. Order
1KG
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-09
Hangzhou Meiya Pharmaceutical Co Ltd
Product
Aprotinin (APT) 9087-70-1
Price
US $0.00-0.00/kg
Min. Order
0.1kg
Purity
≥90.0%
Supply Ability
10 tons
Release date
2024-02-06
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Aprotinin 9087-70-1
Price
US $0.00-0.00/g
Min. Order
1g
Purity
97%
Supply Ability
1kg/month
Release date
2021-05-31

9087-70-1, AprotininRelated Search:

Proteinase K ENTEROKINASE Trypsin Chymotrypsin Pepstatin TYR-D-ALA-GLY-PHE-LEU Glycyl-L-prolyl-L-arginyl-L-prolyl-L-alaninamide Pentapeptide-25 TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Aluminum acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) COBALT(II) ACETYLACETONATE Benzyl isocyanide Ferric acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III) TERT-BUTYL ISOCYANIDE Cupric acetylacetonate KUNITZ SOYBEAN TRYPSIN INHIBITOR,IMMOBILIZED SOYBEAN TRYPSIN INHIBITOR,SOYBEAN TRYPSIN INHIBITOR

  • TRYPSIN INHIBITOR, PANCREAS TYPE
  • TRYPSIN INHIBITOR (BASIC)
  • TRYPSIN INHIBITOR, BOVINE LUNG
  • TRASYLOL
  • antikrein
  • antilysin
  • antilysine
  • aprotininfrombovinelung
  • basicpancreatictrypsininhibitor
  • RecoMbinant aprotinin
  • APROTININ-AGAROSE
  • APROTININ, BOVINE
  • APROTININ, BOVINE AGAROSE CONJUGATE
  • APROTININ BOVINE LUNG
  • Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free - CAS 9087-70-1 - Calbiochem
  • Aprotinin froM bovine lung, ca. 140 U/Mg lyophil.
  • Monoclonal Anti-Acetylated Lysine antibody produced in mouse
  • Anti-Acetylated Lysine: Biotin
  • Anti-Acetylated Lysine-Biotin antibody produced in rabbit
  • Anti-Acetylated Lysine antibody produced in rabbit
  • Aprotinin from bovine lung, >=5400 KIU/mg
  • EZNA KIT ENDO-FREE PLASMID GIGA
  • H-ARG-PRO-ASP-PHE-CYS-LEU-GLU-PRO-PRO-TYR-THR-GLY-PRO-CYS-LYS-ALA-ARG-ILE-ILE-ARG-TYR-PHE-TYR-ASN-ALA-LYS-ALA-GLY-LEU-CYS-GLN-THR-PHE-VAL-TYR-GLY-GLY-CYS-ARG-ALA-LYS-ARG-ASN-ASN-PHE-LYS-SER-ALA-GLU-ASP-CYS-MET-ARG-THR-CYS-GLY-GLY-ALA-OH
  • EC: 3.4.21.9
  • BPTI
  • APROTININ
  • bayera-128
  • fosten
  • kallikrein-trypsininactivator
  • kirrichter
  • onquinin
  • pancreaticbasictrypsininhibitor
  • pancreatictrypsininhibitor
  • pancreatictrypsininhibitor(kunitz)
  • repulson
  • riker52g
  • rp-9921
  • trazinin
  • trypsininhibitor,pancreaticbasic
  • trypsin-kallikreininhibitor(kunitz)
  • zymofren
  • Aprotinin solution
  • Anti-Acetylated Lysine:HRP
  • Anti-Acetylated Lysine-Peroxidase antibody produced in rabbit
  • APROTININ FROM BOVINE LUNGAFFINITY PURIF IED
  • APROTININ FROM BOVINE LUNG CELL CULTURE TESTED
  • APROTININ FROM BOVINE LUNG, ~8000 U/MG P ROTEIN
  • APROTININ, BOVINE LUNG FOR GELFILTRATION CHROMATOG
  • APROTININ-AGAROSE FROM BOVINE LUNG
  • APROTININ, BOVINE, RECOMBINANT
  • APROTININ FROM BOVINE LUNG, LYOPH.,~7000 U/MG*
  • Aprotinin from bovine lung, ca. 140 U/mg
  • Antilysi.
  • Trypsin Inhibitor, pankreatisch basisch
  • Bovine pancreatic trypsin inhibitor
  • Kunitz protease inhibitor
  • Aids043659
  • Aids-043659