β-N-AcetylglucosaMinidase Property

Density 

1.37[at 20℃]

vapor pressure 

0.004Pa at 25℃

storage temp. 

2-8°C

form 

lyophilized powder

color 

Colorless to light yellow

Water Solubility 

125g/L at 25℃

LogP

-1.3 at 20℃

Safety

Hazard Codes 

B

Risk Statements 

20/22-24-37/38-41-42

Safety Statements 

53-26-36/37/39-45

WGK Germany 

3

F 

10

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P285In case of inadequate ventilation wear respiratory protection.

P304+P341IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P501Dispose of contents/container to..…

β-N-AcetylglucosaMinidase Chemical Properties,Usage,Production

Uses

β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease 1 . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms.

Uses

Bovine kidney β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer′s Disease .

Flammability and Explosibility

Not classified

Biochem/physiol Actions

β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.

Purification Methods

Purify it by chromatography on DEAD-Biogel, hydroxylapatite chromatography and gel filtration through Sephacryl S200. Two isoforms: a hexosaminidase EI with Km 177WM