ChemicalBook > CAS DataBase List > Ribonuclease A

Ribonuclease A

Product Name
Ribonuclease A
CAS No.
9001-99-4
Chemical Name
Ribonuclease A
Synonyms
RP-1;RNASE;RNAase;S-RNase;Rnase I;RNASE A;abs9330;RNAase A;Amphinase;DNase-free
CBNumber
CB4131184
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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Ribonuclease A Property

Melting point:
243 °C (dec.)
alpha 
D25 (per mg N) -0.47° (c = 5)
storage temp. 
-20°C
solubility 
Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride
form 
solid
color 
white
PH
pH : 8.0
biological source
protein from bovine pancreas
Water Solubility 
Soluble in water.
Sensitive 
Moisture Sensitive
Specific Activity
85-140Kunitz units/mg protein
EPA Substance Registry System
Nuclease, ribo- (9001-99-4)
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Safety

Safety Statements 
22-24/25
WGK Germany 
3
RTECS 
RF0760000
F 
10-21
TSCA 
Yes
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
RNASEA-RO
Product name
RNase A
Purity
from bovine pancreas
Packaging
25mg
Price
$60.7
Updated
2025/07/31
Sigma-Aldrich
Product number
RNASEA-RO
Product name
RNase A
Purity
from bovine pancreas
Packaging
100mg
Price
$129
Updated
2025/07/31
Sigma-Aldrich
Product number
R7884
Product name
Ribonuclease B from bovine pancreas
Purity
BioReagent,≥50?Kunitzunits/mgprotein,≥80%(SDS-PAGE)
Packaging
500μg
Price
$50.77
Updated
2025/07/31
Sigma-Aldrich
Product number
R1153
Product name
RNase B Glycoprotein Standard from bovine pancreas
Purity
Proteomics Grade
Packaging
500μg
Price
$338.47
Updated
2025/07/31
Sigma-Aldrich
Product number
55674-M
Product name
RNaseA, Bovine Pancreas
Packaging
1 unit
Price
$392
Updated
2025/07/31
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Ribonuclease A Chemical Properties,Usage,Production

Chemical Properties

Lyophilized

Uses

Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. RNase A, and several homologs, may be useful as cancer therapeutics due to their cytotoxic effects on cancer cells. Rnase A is useful in the elucidation of protein structure and absorbance. RNase A has been used as a model system in the study of computer-aided drug design . Product R2638 has scrambled disulfide bonds and is from bovine pancreas.

Uses

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.

General Description

Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Purification Methods

Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins.

Ribonuclease A Preparation Products And Raw materials

Raw materials

Erythromycin Ammonium sulfate

Preparation Products

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Ribonuclease A Suppliers

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View Lastest Price from Ribonuclease A manufacturers

ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD
Product
Ribonuclease A 9001-99-4
Price
US $2.20-8.80/KG
Min. Order
1KG
Purity
99%
Supply Ability
g-kg-tons
Release date
2025-07-08
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Ribonuclease A 9001-99-4
Price
US $1.00/g
Min. Order
1g
Purity
≥60u/mg
Supply Ability
10kg/month
Release date
2021-06-10
Jiangsu Boquan Biotechnology Co., Ltd.
Product
Ribonuclease A 9001-99-4
Price
US $88.00-81.00/g
Min. Order
5g
Purity
99%
Supply Ability
10KG
Release date
2023-02-21

9001-99-4, Ribonuclease ARelated Search:

Ribonucleic acid Phosphatase, Acid froM Wheat GerM EC 3.1.31.1 Nuclease, streptococcal deoxyribo- Alkaline Phosphatase RIBONUCLEASE H RIBONUCLEASE INHIBITOR DNA, SINGLE STRANDED, IMM. ON CELLULOSE, FROM CALF THYMUS* Recombinant RNasin Tosylmethyl isocyanide METHYL ISOCYANOACETATE COBALT(II) ACETYLACETONATE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Cupric acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III) TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) PHENYLSELENOL Benzyl isocyanide

  • Ribonuclease A froM bovine pancreas Min. 70 Kunitz units/Mg lyophil.
  • Ribonuclease A froM bovine pancreas Min. 80 Kunitz units/Mg lyophil.
  • Ribonuclease A froM bovine pancreas(Purified)
  • Ribonuclease A from bovine pancreas Vetec(TM) reagent grade, 80%
  • bonuclease A
  • O-PHENYLENEDIAMINE REAGENT GRADE
  • RIBONUCLEASE A FROM BOVINE PANCREAS, POW DER, ~80 U/MG
  • RIBONUCLEASE A SOLUTION MOLECULAR*BIOLOG Y REAGENT
  • RNASE A, BOVINE PANCREAS
  • RibonucleaseDExBovinePancreas
  • RibonucleaseExBovinePancreas
  • RibonucleaseAExBovinePancreas
  • DNase-free
  • Ribonuclease A from bovine pancreas ca. 70 Kunitz units/mg
  • Ribonuclease A from bovine pancreas ca. 90 Kunitz units/mg
  • ribonuclease s from bovine pancreas
  • rnase b glycoprotein standard from bovine pancreas
  • RNASEA,LYOPHILIZEDPOWDER,HIGHPURITY
  • RP-1
  • Amphinase
  • S-RNase
  • RNase A (from bovine pancreas)
  • RIBONUCLEASE extrapure
  • RibonucleaseA, with scrambled disulfide bonds from bovine pancreas
  • Ribonuclease A-agarose
  • Ribonuclease I, Ribonucleate 3μ-pyrimidinooligonucleotidohydrolase
  • Rnase I
  • ribornuclease
  • RNAase
  • RIBONUCLEASE A FROM BOVINEPANCREAS CA.90 KUNITZ-U/MG LYOPHIL.
  • RIBONUCLEASE A BOVINE PANCR.CA.70 KUNITZ-U/MG LYOPHIL.
  • RNase B Glycoprotein Standard from bovine pancreas,RNase B, Ribonuclease B from bovine pancreas
  • Ribonuclease A from bovine pancreas,Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
  • Ribonuclease A, from bovine pancreas, 50 KU/mg
  • RIBONUCLEASE A FROM BOVINE PANCREAS, POW DER, ~100 U/MG
  • RIBONUCLEASE B PROTEOMICS GRADE
  • RIBONUCLEASE A TYPE X-A: SOLUTION FROM*B OVINE PANCR
  • RIBONUCLEASE A FROM BOVINE PANCREAS, IN 0.2 M SO-PHOSPHATE*
  • RIBONUCLEASE A FROM BOVINE PANCREAS, >70 U/MG*
  • RIBONUCLEASE A FROM BOVINE PANCREAS, TYPE 1-A
  • Ribonuclease A from bovine pancreas, lyophil.
  • ribonuclease A solution molecular*biology
  • ribonuclease A type I-A from*bovine pancreas
  • ribonuclease A type I-as from*bovine pancreas
  • ribonuclease A type ii-A from*bovine pancreas
  • ribonuclease A type iii-A from*bovine pancreas
  • ribonuclease A type X-A: solution from*bovine pan
  • ribonuclease A type xii-A from bovine*pancreas
  • Ribonuclease A, Protease-Free
  • ribonuclease from aspergillus clavatus
  • ribonuclease S grade xii-S
  • Nuclease, ribo-
  • RIBONUCLEASE A, CHROMATOGRAPHICALLY*PURI FIED, MOLEC
  • RIBONUCLEASE A FROM BOVINE PANCREAS 4XCR SALTFREE ~70 U/MG
  • RIBONUCLEASE A FROM BOVINE PANCREAS, ~90 U/MG
  • Ribonuclease B from bovine pancreas,RNase B
  • Ribonuclease from Bovine Pancreas
  • Ribonuclease A from bovine pancreas min. 80 Kunitz units/mg