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CHYMOSTATIN

Product Name
CHYMOSTATIN
CAS No.
9076-44-2
Chemical Name
CHYMOSTATIN
Synonyms
AIDS-028443;CHYMOSTATIN;CHYMOSTATIN USP/EP/BP;ChyMostatin research grade;ACRYLAMIDE PROTEOMICS GRADE;Chymostatin from Microbial Source;chymostatin, mixture of components A;N-[-N-ALPHA-CARBONYL-CPD-X-PHE-AL]-PHE;Chymostatin - CAS 9076-44-2 - Calbiochem;CHYMOSTATIN, MIXTURE OF COMPONENTS A, B AND C
CBNumber
CB4161466
Molecular Formula
C92H121N21O18
Formula Weight
1809.08
MOL File
9076-44-2.mol
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CHYMOSTATIN Property

Melting point:
205 °C
storage temp. 
-20°C
solubility 
DMSO: 10 mM Stock solutions stable for months at −20 °C.
form 
White to slightly yellow solid or powder
color 
White or off-white
biological source
microbial
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-24/25-36-26
WGK Germany 
3
RTECS 
GC3047700
10-21
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
C7268
Product name
Chymostatin
Purity
microbial
Packaging
1mg
Price
$67.5
Updated
2025/07/31
Sigma-Aldrich
Product number
C7268
Product name
Chymostatin
Purity
microbial
Packaging
5mg
Price
$173
Updated
2025/07/31
Sigma-Aldrich
Product number
230790
Product name
Chymostatin - CAS 9076-44-2 - Calbiochem
Purity
Contains chymostatin "A" as a major form.
Packaging
5mg
Price
$148
Updated
2025/07/31
Sigma-Aldrich
Product number
230790
Product name
Chymostatin - CAS 9076-44-2 - Calbiochem
Purity
Contains chymostatin "A" as a major form.
Packaging
25mg
Price
$474
Updated
2025/07/31
Sigma-Aldrich
Product number
11004638001
Product name
Chymostatin
Packaging
10mg
Price
$288
Updated
2025/07/31
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CHYMOSTATIN Chemical Properties,Usage,Production

Description

Chymostatin is a bioactive peptide of microbial origin that acts as a protease inhibitor with selectivity for chymotryptase-like serine proteases. It potently inhibits chymotrypsin and chymase (Ki = 9.36 and 13.1 nM, respectively) while less effectively blocking the activity of cathepsins, papain, and leukocyte elastase. It is without effect on trypsin, thrombin, plasmin, pepsin, and kallikrein.

Uses

Chymostatin has been used in a study that determined that molecular calculations are useful for evaluating the interactions between ligands, including inhibitors and homologous enzymes, in docking models. Chymostatin has also been used in a study to investigate the norovirus protease as an attractive target for antiviral drug development.

Uses

Chymostatin is a specific inhibitor of α-, β-, γ-, and δ-chymotrypsin.

General Description

Chymostatin is a mixture of three components, A, B, and C. The component A being N-[((S)-1-carboxy-2-phenylethyl)-carbamoyl]-α-[2-iminohexahydro-4(S)-pyrimidyl]-L-glycyl-L-leucyl-phenylalaninal. The other two components B and C differ in that the L-leucyl residue is substituted by L-valine and L-isoleucine, respectively.

Biochem/physiol Actions

Chymostatin is a strong inhibitor of many proteases, including chymotrypsin, papain, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases such as cathepsins A,B,C, B, H, and L. It weakly inhibits human leucocyte elastase. It is effective at a final concentration of 100 to 200 μg/ml (10 to 100 μM). Chymostatin is often included in protease inhibitor cocktails used with plant extracts.

in vitro

chymostatin was found to be a potent inhibitor of human leucocyte chymotrypsin-like protease. its affinity to the leucocyte protease was found to be much higher than its affinity to bovine pancreatic alpha-chymotrypsin. chymsotatin also showed a weak inhibitory effect on the activity of human leucocyte elastase. in addition, the preincubation of chymostatin with 35so2-4-labeled cartilage before the addition of the human chymotrypsin-like protease to the tissue could also inhibit 35so2-4 release [1].

in vivo

the effect of chymostatin on c57bl/6j-dy dystrophic mice was studied. the locomotor activity of normal mice markedly increased, attaining a plateau at 8 weeks of age. serum levels of creatine phosphokinase were much higher in dystrophic mice when compared with normal mice, and dystrophic mice also showed a reduced muscle protein content. when 3-week-old dystrophic mice received chymostatin at 1 mg/kg, the decrease in locomotor activity could be retarded, serum enzyme levels significantly decreased, and muscle protein content also obviously increased. in addition, the survival time of chymostatin-treated dystrophic mice was prolonged [2].

References

[1] H UMEZAWA. Chymostatin, a new chymotrypsin inhibitor produced by actinomycetes.[J]. Journal of Antibiotics, 1970, 23 8: 425-427. DOI:10.7164/antibiotics.23.425
[2] LAMBRO A. JOHNSON  Mark E  Kenneth E Moon. Inactivation of chymotrypsin and human skin chymase: Kinetics of time-dependent inhibition in the presence of substrate[J]. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988, 953: Pages 269-279. DOI:10.1016/0167-4838(88)90035-0
[3] ROSS L. STEIN  Anne M S. Slow-binding inhibition of chymotrypsin and cathepsin G by the peptide aldehyde chymostatin[J]. Biochemistry Biochemistry, 1987, 26 9: 2611-2615. DOI:10.1021/bi00383a030
[4] MALWINA MONIKA ROSZKOWSKA-CHOJECKA. Effects of chymostatin, a chymase inhibitor, on blood pressure, plasma and tissue angiotensin II, renal haemodynamics and renal excretion in two models of hypertension in the rat[J]. Experimental Physiology, 2015, 100 9: 1093-1105. DOI:10.1113/ep085325

CHYMOSTATIN Preparation Products And Raw materials

Raw materials

Preparation Products

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CHYMOSTATIN Suppliers

J & K SCIENTIFIC LTD.
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18210857532; 18210857532
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86-10-82849933
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jkinfo@jkchemical.com
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China
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96815
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Alabiochem Tech.Co., Ltd.
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512-58900862 400-0707518
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0086-512-56765862
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sales@alabiochem.com
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China
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Shanghai Hanhong Scientific Co.,Ltd.
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021-54306202 13764082696
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info@hanhongsci.com
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China
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Chemsky (shanghai) International Co.,Ltd
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021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
15402
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Shandong Xiya Chemical Co., Ltd
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4009903999 13355009207
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0539-6365991
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3007715519@qq.com
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China
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Sichuan Kulinan Technology Co., Ltd
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400-1166-196 18981987031
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028-84555506 800101999
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cdhxsj@163.com
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Hunan Hui Bai Shi Biotechnology Co., Ltd.
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0731-85526065 13308475853
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Spectrum Chemical Manufacturing Corp.
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021-021-021-67601398-809-809-809 15221380277
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021-57711696
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marketing_china@spectrumchemical.com
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China
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Beijing HuaMeiHuLiBiological Chemical
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010-56205725
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010-65763397
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waley188@sohu.com
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China
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Shanghai Aladdin Bio-Chem Technology Co.,LTD
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400-6206333 13167063860
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021-50323701
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anhua.mao@aladdin-e.com
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China
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View Lastest Price from CHYMOSTATIN manufacturers

Career Henan Chemical Co
Product
CHYMOSTATIN 9076-44-2
Price
US $1.00/KG
Min. Order
1KG
Purity
98% HPLC
Supply Ability
10 tons
Release date
2020-03-04

9076-44-2, CHYMOSTATINRelated Search:


  • (S)-1-CARBOXY-2-PHENYLETHYL]-CARBAMOYL-ALPHA-[2-AMIDOHEXAHYDRO-4(S)-PYRIMIDYL]-(S)-GLYCYL-[A = LEU; B = VAL; OR C = ILE]-PHENYLALANINAL
  • (S)-1-CARBOXY-2-PHENYLETHYL)-CARBAMOYL-ALPHA-[2-IMINOHEXAHYDRO-4(S)-PYRIMIDYL]-(S)-GLYCYL-X-PHENYLALANINAL
  • [(S)-1-CARBOXY-2-PHENYLETHYL]-CARBAMOYL-ALPHA-[2-IMINOHEXAHYDRO-4(S)-PYRIMIDYL]-(S)-GLY-X-PHE-AL
  • N-[-N-ALPHA-CARBONYL-CPD-X-PHE-AL]-PHE
  • CHYMOSTATIN
  • AIDS-028443
  • ChyMostatin research grade
  • ACRYLAMIDE PROTEOMICS GRADE
  • Chymostatin from Microbial Source
  • chymostatin, mixture of components A
  • CHYMOSTATIN, MIXTURE OF COMPONENTS A, B AND C
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  • Chymostatin - CAS 9076-44-2 - Calbiochem
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  • CHYMOSTATIN USP/EP/BP
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  • BioChemical
  • Biochemicals and Reagents
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