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Disodium 4-nitrophenylphosphate

Product Name
Disodium 4-nitrophenylphosphate
CAS No.
4264-83-9
Chemical Name
Disodium 4-nitrophenylphosphate
Synonyms
PNPP;4-NPP;sodiuM 4-nitrophenyl phosphate;4-PNPP;ABTS<PNPP-NA2;PNPP, 2NA;2-oxazole;PNPP di-NA;PNP-PHOS DINA
CBNumber
CB4219815
Molecular Formula
C6H7NNaO6P
Formula Weight
243.09
MOL File
4264-83-9.mol
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Disodium 4-nitrophenylphosphate Property

Melting point:
>300 °C(lit.)
Density 
1.751 at 20℃
vapor pressure 
15Pa at 20-50℃
storage temp. 
-20°C
solubility 
H2O: 1 tablet/3 mL 25 mM, clear, light yellow
form 
tablets
color 
white to pale yellow
Water Solubility 
Miscible with water. Immiscible with ethanol and acetone.
BRN 
5324661
Stability:
Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.
InChIKey
KAKKHKRHCKCAGH-UHFFFAOYSA-L
LogP
-2.16 at 22℃ and pH6.7
Surface tension
69.34mN/m at 1.051g/L and 20℃
CAS DataBase Reference
4264-83-9(CAS DataBase Reference)
EPA Substance Registry System
Phosphoric acid, mono(4-nitrophenyl) ester, disodium salt (4264-83-9)
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Safety

Hazard Codes 
Xi,Xn
Risk Statements 
36/38-36/37/38-48/22-41-38-21/22
Safety Statements 
26-36-24/25-23-36/37/39
WGK Germany 
3
F 
10-21
TSCA 
Yes
HS Code 
29199000
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H290May be corrosive to metals

H315Causes skin irritation

H318Causes serious eye damage

H373May cause damage to organs through prolonged or repeated exposure

Precautionary statements

P202Do not handle until all safety precautions have been read and understood.

P234Keep only in original container.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P308+P313IF exposed or concerned: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
4876
Product name
p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate - CAS 4264-83-9 - Calbiochem
Purity
p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM).
Packaging
1g
Price
$47.8
Updated
2025/07/31
Sigma-Aldrich
Product number
N7653
Product name
p-Nitrophenyl Phosphate Liquid Substrate System
Purity
liquid
Packaging
100ml
Price
$145
Updated
2025/07/31
Sigma-Aldrich
Product number
4876
Product name
p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate - CAS 4264-83-9 - Calbiochem
Purity
p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM).
Packaging
5G
Price
$171
Updated
2025/07/31
TRC
Product number
P653443
Product name
PNPP,DisodiumHexahydrate
Packaging
1g
Price
$65
Updated
2021/12/16
Frontier Specialty Chemicals
Product number
JK1084029
Product name
4-Nitrophenyl phosphate disodium salt
Purity
98%
Packaging
1g
Price
$13
Updated
2021/12/16
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Disodium 4-nitrophenylphosphate Chemical Properties,Usage,Production

Chemical Properties

White crystalline

Uses

PNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures.

General Description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.

Biochem/physiol Actions

Primary TargetSubstrate for phosphatase

Purification Methods

Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.]

Disodium 4-nitrophenylphosphate Preparation Products And Raw materials

Raw materials

Phosphorus oxitrichloride 4-Nitrophenol

Preparation Products

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Disodium 4-nitrophenylphosphate Suppliers

Austria 1 Belgium 1 Canada 1 China 332 Czech Republic 1 Europe 2 France 1 Germany 5 India 17 Japan 3 South Korea 1 Switzerland 2 United Kingdom 11 United States 37 Global 415
Nanjing UltraPure Biotech Co., Ltd.
Tel
025-82263158 13851993283
Fax
025-82263168
Email
2842979086@qq.com
Country
China
ProdList
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58
NANTONG XINHE BIOTECH CO.,LTD
Tel
13813727166，15370460228; 13813727166
Email
sale_xinhe@outlook.com
Country
China
ProdList
374
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Chongqing Xingcan Pharmaceutical Technology Co., Ltd.
Tel
023-13650506873 19923294409
Email
xingcanyaoye@sina.com
Country
China
ProdList
1604
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Hunan Waybel Advanced Materials Co.,Ltd
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0731-85529965 13319523173
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3002708854@qq.com
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China
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Hunan Hui Bai Shi Biotechnology Co., Ltd.
Tel
0731-85526065 13308475853
Email
ivy@hnhbsj.com
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China
ProdList
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Shandong Kangsaide Biological Technology Co. LTD
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13475906826 18615616891
Email
zhangdong.0602@163.com
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China
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58
Guangzhou Yaoguang Technology Co., Ltd.
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13020159086
Email
yangjue@ygfuture.com
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China
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424
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Hunan Hui Bai Yi New Materials Co., Ltd
Tel
0745-2866551 19976851779
Email
3002713163@qq.com
Country
China
ProdList
7333
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58
J & K SCIENTIFIC LTD.
Tel
18210857532; 18210857532
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
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76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
4006356688 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
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View Lastest Price from Disodium 4-nitrophenylphosphate manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Disodium 4-nitrophenylphosphate 4264-83-9
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
3500kg/month
Release date
2021-09-14
Hebei Chuanghai Biotechnology Co., Ltd
Product
Disodium 4-nitrophenylphosphate 4264-83-9
Price
US $10.00/KG
Min. Order
100KG
Purity
99%
Supply Ability
100 mt
Release date
2024-11-21
Zhuozhou Wenxi import and Export Co., Ltd
Product
Disodium 4-nitrophenylphosphate 4264-83-9
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-10

4264-83-9, Disodium 4-nitrophenylphosphateRelated Search:

PHOSPHATE STANDARD Diphenyl chlorophosphate Triethyl phosphate Trisodium phosphate Pamidronate disodium salt Dibenzyl phosphate Sodium Phosphate, Dibasic Diphenylphosphoryl azide Tylosin phosphate 4-Nitrobenzoic acid Ethylenediaminetetraacetic acid disodium salt Starch, hydrogen phosphate, 2-hydroxypropyl ether 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Diphenyl phosphate AMPHISOL A Zinc phosphate Clodronate disodium Salt Sodium Monofluorophosphate

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