Coumarin 151 Property

Melting point:

221-222 °C(lit.)

Boiling point:

314.6±42.0 °C(Predicted)

Density 

1.4096 (estimate)

storage temp. 

under inert gas (nitrogen or Argon) at 2–8 °C

pka

1.12±0.20(Predicted)

form 

Powder

color 

Yellow

Water Solubility 

Soluble in dimethyl sulfoxide (25 mg/ml), dimethyl formamide (25 mg/ml), and methanol (25 mg/ml). Insoluble in water.

BRN 

4456797

CAS DataBase Reference

53518-15-3(CAS DataBase Reference)

EPA Substance Registry System

2H-1-Benzopyran-2-one, 7-amino-4-(trifluoromethyl)- (53518-15-3)

Safety

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22

Safety Statements 

26-36-37/39

WGK Germany 

3

TSCA 

T

HazardClass 

IRRITANT

HS Code 

29322090

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Coumarin 151 Chemical Properties,Usage,Production

Chemical Properties

Coumarin 151 is yellow powder

Uses

Coumarin 151 is used to prepare peptidase substrates and for the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids.

Uses

7-Amino-4-(trifluoromethyl)coumarin is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids. It is suitable as laser dye.

Definition

ChEBI: 7-amino-4-(trifluoromethyl)coumarin is a member of 7-aminocoumarins. It has a role as a fluorochrome.

Purification Methods

Purify the coumarin by column chromatography on a C18 column, elute with acetonitrile/0.01M H2O/HCl (1:1), and recrystallise it from isopropanol. Alternatively, it is eluted from a silica gel column with CH2Cl2, or by extracting a CH2Cl2 solution (4g/L) with 1M aqueous NaOH (3 x 0.1L), followed by drying (MgSO4), filtration and evaporation. [Bissell J Org Chem 45 2283 1980, Zimmermann et al. Anal Biochem 70 258 1976.]