ChemicalBook > CAS DataBase List > 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Product Name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
CAS No.
6578-06-9
Chemical Name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Synonyms
BCIP;BClP;BCIP-T;abs9176;BCIP P-TOL;X-PHOS P-TOL;BCIP-TOLUIDINE;BCIP P-TOLUIDINE;BCIP/INT SUBSTRATE;BCIP p-toluidine sal
CBNumber
CB4854083
Molecular Formula
C8H6BrClNO4P.C7H9N
Formula Weight
433.63
MOL File
6578-06-9.mol
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Property

Melting point:
194-195℃
storage temp. 
-20°C
solubility 
DMF: 20 mg/mL
form 
tablets
color 
White to Orange to Green
InChIKey
QEIFSLUFHRCVQL-UHFFFAOYSA-N
CAS DataBase Reference
6578-06-9(CAS DataBase Reference)
EPA Substance Registry System
1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38-40
Safety Statements 
26
RIDADR 
2811
WGK Germany 
3
F 
3-8-10
HazardClass 
6.1
PackingGroup 
III
HS Code 
29339190
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H226Flammable liquid and vapour

H319Causes serious eye irritation

Precautionary statements

P201Obtain special instructions before use.

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P370+P378In case of fire: Use … for extinction.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
BCIPP-RO
Product name
BCIP
Purity
5-bromo-4-chloro-3-indolyl-phosphate,4-toluidinesalt
Packaging
250mg
Price
$208
Updated
2024/03/01
Sigma-Aldrich
Product number
BCIPP-RO
Product name
BCIP
Purity
5-bromo-4-chloro-3-indolyl-phosphate,4-toluidinesalt
Packaging
1g
Price
$453
Updated
2024/03/01
Sigma-Aldrich
Product number
B0274
Product name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Purity
tablet
Packaging
10tablets
Price
$399
Updated
2024/03/01
Sigma-Aldrich
Product number
B0274
Product name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Purity
tablet
Packaging
25tablets
Price
$794
Updated
2024/03/01
Sigma-Aldrich
Product number
B6777
Product name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Purity
BioReagent, for molecular biology, powder, ≥99%
Packaging
100MG
Price
$121
Updated
2023/06/20
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Chemical Properties,Usage,Production

Chemical Properties

White to light brown powder

Uses

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

General Description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Preparation Products And Raw materials

Raw materials

Preparation Products

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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Suppliers

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View Lastest Price from 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt manufacturers

Henan Fengda Chemical Co., Ltd
Product
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt 6578-06-9
Price
US $10.00-1.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-08
WUHAN FORTUNA CHEMICAL CO., LTD
Product
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt 6578-06-9
Price
US $0.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
3500kg/month
Release date
2021-09-14
Zhuozhou Wenxi import and Export Co., Ltd
Product
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt 6578-06-9
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-10

6578-06-9, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine saltRelated Search:

AMPHISOL A BCDMH 4-Methylaniline hydrochloride Trisodium phosphate Calcium phosphate Chlorobromoisocyanurate Sodium Phosphate Monobasic Monohydrate p-Toluidine CHLORODIBROMOMETHANE Tributyl phosphate phosphate 8-CHLOROADENOSINE-3',5'-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Bromine BCIP,BCIP 2 K,BCIP DIPOTASSIUM SALT 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALT X-PHOS BCIP/NBT toluidine

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  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT
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  • 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Gel Electrophoresis]
  • Phosphoric acid 4-chloro-5-bromo-1H-indole-3-yl·p-toluidine
  • (BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine
  • 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research]
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUI
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  • 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPH.P-TOLUIDINE-SALT RES.GRADE
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  • BROMO-4-CHLORO-3-INDOLYL PHOSPHATE MONO-(-TOLUIDINIUM) SALT
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  • BCIP 4-TOLUIDINE SALT
  • BCIP-TOLUIDINE
  • BCIP PARA-TOLUIDINE SALT
  • BCIP P-TOL
  • BCIP P-TOLUIDINE
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  • BCIP-T
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  • BCIP p-toluidine sal
  • (3R,2'R)-2,2-Difluoro-3-[methyl-(2-methyl-propane-2-sulfinyl)-amino]-3-(4-nitro-phenyl)-propionic
  • Phosphoric acid-Mono-(5-broMo-4-chloro-1H-indol-3-yl) ester · p-toluidine, BCIP · p-toluidine, X-Phosphate · p-toluidine
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT (BCIP P-TOLUIDINE SALT)
  • 5-BroMo-4-Bhloro-3-Indolylphosphate4-toluidinesalt
  • Toluidine blue(BCIP)
  • BroMo-4-chloro-3-indolyl-phosphate-p-toluidine-salt (BCIP) research grade
  • X-PHOSPHATE P-TOLUIDINE SALT
  • X-PHOS P-TOL
  • X-PHOS P-TOLUIDINE SALT
  • 1h-indol-3-ol,5-bromo-4-chloro-,dihydrogenphosphate(ester),compd.with4-m
  • ethylbenzenamine(1:1)
  • PHOSPHORIC ACID-MONO-(5-BROMO-4-CHLORO-1H-INDOL-3-YL) ESTER P-TOLUIDINE
  • 5-bromo-4-chloro-3-indolyl hydrogen phosphate--p-toluidine (1:1)
  • X-Phos-p-tol 5-Bromo-4-chloro-3-indolyl phosphate, p-toluidine salt
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATEP-TO LUIDINE 25
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  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATEP-TO LUIDINE MO
  • Bcip,P-ToluidineSalt(5-Bromo-4-Chloro-3-IndoylPhosphate)
  • BCIP 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE
  • 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1)
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT MOLECULAR BIOLOGY REAGENT 98+% BCIP
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  • 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt >99%
  • X-Phos p-toluidine salt, BCIP p-toluidine salt
  • 1H-Indol-3-ol, 5-bromo-4-chloro-, 3-(dihydrogen phosphate), compd. with 4-methylbenzenamine
  • BClP
  • ACETIC ACID GLACIAL ACS GRADE
  • 5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt, micronized
  • 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP,P-toluidien salt)
  • p-Toluidine 5-bromo-4-chloro-1H-indol-3-yl phosphate