ChemicalBook > CAS DataBase List > Anistreplasa [INN-Spanish]

Anistreplasa [INN-Spanish]

Product Name
Anistreplasa [INN-Spanish]
CAS No.
81669-57-0
Chemical Name
Anistreplasa [INN-Spanish]
Synonyms
APSAC;Eminase;BRL 26921;Anistreplase;Anistreplasa [INN-Spanish];Anistreplasa [INN-Spanish] USP/EP/BP
CBNumber
CB51245025
Formula Weight
0
MOL File
Mol file
More
Less

Hazard and Precautionary Statements (GHS)

More
Less

Anistreplasa [INN-Spanish] Chemical Properties,Usage,Production

Description

APSAC is an anisoylated human plasminogen-streptokinase complex useful in the treatment of myocardial infarct. Its reported advantages over other existing thrombolytics such as alteplase and streptokinase include a longer half life, ease of administration, and a more specific local antithrombotic effect.

Originator

Beecham (United Kingdom)

Uses

Fibrinolytic.

Manufacturing Process

Preparation of freeze dried p-anisoyl streptokinase/plasminogen complex without internal peptide bond cleavages:
Streptokinase (250,000 units, Kabi, Stockholm, Sweden) was dissolved in 0.1 M trishydroxymethylmethane hydrochloride pH 7.4 (2.5 ml) and a solution of 0.1 M p-amidinophenyl p'-anisate in dimethylsulphoxide (0.25 ml) added. A slight cloudiness resulted. To this mixture was added 0.5 ml of a solution of human lys-plasminogen (8.99 mg/ml in the above buffer) [Kabi, Stockholm] and the solution was thoroughly and rapidly mixed. After standing on ice for 15 minutes, the solution was stored on ice until use. After approximaely 2 hours 0.4 ml (40,000 units) of the material was diluted with 3.0 ml of a slurry of L-lysine-sepharose 4B (a 33% wet wt/volume suspension in the above buffer) and stood at 0°C for 1 hour. The gel was filtered on a glass sinter funnel at 4°C under suction and washed with the buffer (100 ml). The gel was eluted under gentle suction with the same buffer containing 0.1 M ε- aminocaproic acid (3 lots of 5 ml). The combined filtrates were dialysed for 2 hours at 4°C against ammonium bicarbonate buffer (50 mM pH 7.0) containing 2.5% w/v mannitol. The product was then freeze dried to 0.799 g of a white solid. Approximately 195 mg of this material was analysed by slab gel polyacrylamide gel electrophoresis using 10% w/v gels in the presence of sodium dodecyl sulphate. Two main polypeptide bands were observed corresponding to lys-plasminogen (m.w. 84,000) and streptokinase (m.w. 47,000). There was also a trace of contaminating human serum albumin derived from the commercial streptokinase preparation.

brand name

Eminase

Therapeutic Function

Thrombolytic

Anistreplasa [INN-Spanish] Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

Anistreplasa [INN-Spanish] Suppliers

LGM Pharma
Tel
1-(800)-881-8210
Fax
615-250-9817
Email
inquiries@lgmpharma.com
Country
United States
ProdList
2123
Advantage
70
Beijing HuaMeiHuLiBiological Chemical
Tel
010-56205725
Fax
010-65763397
Email
waley188@sohu.com
Country
China
ProdList
12335
Advantage
58
Dideu Industries Group Limited
Tel
+86-29-89586680 +86-15129568250
Email
1026@dideu.com
Country
China
ProdList
22784
Advantage
58
Hubei xin bonus chemical co. LTD
Tel
86-13657291602
Fax
027-59338440
Email
linda@hubeijusheng.com
Country
CHINA
ProdList
22963
Advantage
58