AMINOPEPTIDASE Property

storage temp. 

-20°C

form 

lyophilized powder

Safety

WGK Germany 

1

HS Code 

3504009000

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

AMINOPEPTIDASE Chemical Properties,Usage,Production

Uses

Aminopeptidase I from Streptomyces griseus has been used:

• to test the biochar exposure effect on the enzyme activity
• in circular dichroism (CD) spectroscopy studies
• as a positive control in p-nitroanilide degradation assay

General Description

Aminopeptidase I from Streptomyces griseus is a thermostable enzyme with Glu131 and Tyr246 as key active site residues.

Biochem/physiol Actions

Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease.

Purification Methods

The aminopeptidase is purified about 2000-fold by column chromatography on CM-cellulose, hydroxylapatite and Gly-Pro AH-Sepharose. [Imai et al. J Biochem (Tokyo) 93 431 1983, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 6 p 286 2002.] DNA (deoxyribonucleic acids). The essential structures of chromosomes are DNA and contain the genetic “blueprint” in the form of separate genes. They are made up of the four deoxyribonucleic acids (nucleotides): adenylic acid, guanylic acid, cytidylic acid and thymidylic acid (designated A, G, C, T respectively) linked together by their phosphate groups in ester bonds between the 3' and 5' hydroxy groups of the 2'-deoxy-D-ribose moiety of the nucleotides. The chains form a double-stranded spiral (helix) in which the two identical nucleotide sequences run antiparallel with the heterocyclic bases hydrogen bonded (A….T, G….C) forming the “ladder” between the strands. Short sequences of DNA are available commercially, are commercially custom made or synthesised in a DNA synthesiser and purified by HPLC. Their purity can be checked by restriction enzyme cleavage followed by gel electophoresis, or directly by gel electrophoresis or analytical HPLC. Commercial DNAs are usually pure enough for direct use but can be further purified using commercially available kits involving binding to silica or other matrices and eluting with Tris buffers. There are now rapid “throughput” techniques for sequencing DNA which are very accurate.